Recombinant Human Calpain 2 protein (His tag) is a Human Full Length protein, in the 20 to 700 aa range, expressed in Escherichia coli, with >90% purity and suitable for SDS-PAGE.
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Key facts
- Purity
- >90% SDS-PAGE
- Expression system
- Escherichia coli
- Tags
- His tag N-Terminus
- Applications
- SDS-PAGE
- Biologically active
- No
Amino acid sequence
S H D R A I K Y L N Q D Y E A L R N E C L E A G T L F Q D P S F P A I P S A L G F K E L G P Y S S K T R G I E W K R P T E I C A D P Q F I I G G A T R T D I C Q G A L G D C W L L A A I A S L T L N E E I L A R V V P L N Q S F Q E N Y A G I F H F Q F W Q Y G E W V E V V V D D R L P T K D G E L L F V H S A E G S E F W S A L L E K A Y A K I N G C Y E A L S G G A T T E G F E D F T G G I A E W Y E L K K P P P N L F K I I Q K A L Q K G S L L G C S I D I T S A A D S E A I T F Q K L V K G H A Y S V T G A E E V E S N G S L Q K L I R I R N P W G E V E W T G R W N D N C P S W N T I D P E E R E R L T R R H E D G E F W M S F S D F L R H Y S R L E I C N L T P D T L T S D T Y K K W K L T K M D G N W R R G S T A G G C R N Y P N T F W M N P Q Y L I K L E E E D E D E E D G E S G C T F L V G L I Q K H R R R Q R K M G E D M H T I G F G I Y E V P E E L S G Q T N I H L S K N F F L T N R A R E R S D T F I N L R E V L N R F K L P P G E Y I L V P S T F E P N K D G D F C I R V F S E K K A D Y Q A V D D E I E A N L E E F D I S E D D I D D G F R R L F A Q L A G E D A E I S A F E L Q T I L R R V L A K R Q D I K S D G F S I E T C K I M V D M L D S D G S G K L G L K E F Y I L W T K I Q K Y Q K I Y R E I D V D R S G T M N S Y E M R K A L E E A G F K M P C Q L H Q V I V A R F A D D Q L I I D F D N F V R C L V R L E T L F K I F K Q L D P E N T G T I E L D L I S W L C F S V L
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application SDS-PAGE | Reactivity Reacts | Dilution info - | Notes - |
Associated Products
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Target data
Function
Calcium-regulated non-lysosomal thiol-protease which catalyzes limited proteolysis of substrates involved in cytoskeletal remodeling and signal transduction. Proteolytically cleaves MYOC at 'Arg-226' (PubMed:17650508). Proteolytically cleaves CPEB3 following neuronal stimulation which abolishes CPEB3 translational repressor activity, leading to translation of CPEB3 target mRNAs (By similarity).
Alternative names
CANPL2, CAPN2, Calpain-2 catalytic subunit, Calcium-activated neutral proteinase 2, Calpain M-type, Calpain large polypeptide L2, Calpain-2 large subunit, Millimolar-calpain, CANP 2, M-calpain
Recommended products
Recombinant Human Calpain 2 protein (His tag) is a Human Full Length protein, in the 20 to 700 aa range, expressed in Escherichia coli, with >90% purity and suitable for SDS-PAGE.
Key facts
- Purity
- >90% SDS-PAGE
- Expression system
- Escherichia coli
- Tags
- His tag N-Terminus
- Applications
- SDS-PAGE
- Biologically active
- No
- Accession
- P17655-1
- Animal free
- No
- Species
- Human
- Concentration
- Storage buffer
Constituents: Tris buffer, 50% Glycerol (glycerin, glycerine)
Sequence info
Amino acid sequence
- S H D R A I K Y L N Q D Y E A L R N E C L E A G T L F Q D P S F P A I P S A L G F K E L G P Y S S K T R G I E W K R P T E I C A D P Q F I I G G A T R T D I C Q G A L G D C W L L A A I A S L T L N E E I L A R V V P L N Q S F Q E N Y A G I F H F Q F W Q Y G E W V E V V V D D R L P T K D G E L L F V H S A E G S E F W S A L L E K A Y A K I N G C Y E A L S G G A T T E G F E D F T G G I A E W Y E L K K P P P N L F K I I Q K A L Q K G S L L G C S I D I T S A A D S E A I T F Q K L V K G H A Y S V T G A E E V E S N G S L Q K L I R I R N P W G E V E W T G R W N D N C P S W N T I D P E E R E R L T R R H E D G E F W M S F S D F L R H Y S R L E I C N L T P D T L T S D T Y K K W K L T K M D G N W R R G S T A G G C R N Y P N T F W M N P Q Y L I K L E E E D E D E E D G E S G C T F L V G L I Q K H R R R Q R K M G E D M H T I G F G I Y E V P E E L S G Q T N I H L S K N F F L T N R A R E R S D T F I N L R E V L N R F K L P P G E Y I L V P S T F E P N K D G D F C I R V F S E K K A D Y Q A V D D E I E A N L E E F D I S E D D I D D G F R R L F A Q L A G E D A E I S A F E L Q T I L R R V L A K R Q D I K S D G F S I E T C K I M V D M L D S D G S G K L G L K E F Y I L W T K I Q K Y Q K I Y R E I D V D R S G T M N S Y E M R K A L E E A G F K M P C Q L H Q V I V A R F A D D Q L I I D F D N F V R C L V R L E T L F K I F K Q L D P E N T G T I E L D L I S W L C F S V L
- Accession
- P17655
- Protein length
- Full Length
- Predicted molecular weight
- 82.1 kDa
- Amino acids
- 20 to 700
- Nature
- Recombinant
- Tags
- His tag N-Terminus
Specifications
- Form
- Liquid
General info
- Function
Calcium-regulated non-lysosomal thiol-protease which catalyzes limited proteolysis of substrates involved in cytoskeletal remodeling and signal transduction. Proteolytically cleaves MYOC at 'Arg-226' (PubMed:17650508). Proteolytically cleaves CPEB3 following neuronal stimulation which abolishes CPEB3 translational repressor activity, leading to translation of CPEB3 target mRNAs (By similarity).
- Sequence similarities
Belongs to the peptidase C2 family.
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Calpain 2 also known as CAPN2 or calpain-2 is a heterodimer protease enzyme weighing around 80 kDa. It belongs to the calpain family which requires calcium ions for activation. Calpain 2 expresses in many tissues including the brain muscles and liver indicating its widespread role in cellular processes. The enzyme comprises a large catalytic subunit and a small regulatory subunit each encoded by different genes.
- Biological function summary
Calpain-2 plays a significant role in cell signaling cytoskeletal remodeling and apoptosis regulation. It does not typically function in isolation but partners with other molecules forming complexes necessary for precise proteolytic activity. This enzyme modulates several cell functions by cleaving substrates like cytoskeletal proteins transcription factors and enzymes which allows cells to respond adaptively to various stimuli.
- Pathways
Calpain-2 integrates into important signaling cascades such as the ERK and PI3K/AKT pathways. It influences these pathways by processing specific proteins and modifying their activity thereby affecting cell survival and proliferation. Calpain-2 closely interacts with proteins like phosphatidylinositol-45-bisphosphate 3-kinase (PI3K) and extracellular signal-regulated kinases (ERKs) within these pathways emphasizing its importance in finely-tuning cellular responses.
- Associated diseases and disorders
Calpain-2 links to several pathological conditions including neurodegenerative diseases like Alzheimer's and muscular disorders such as Duchenne Muscular Dystrophy. In Alzheimer's disease calpain-2 activity influences the processing of amyloid precursor proteins contributing to plaque formation. In Duchenne Muscular Dystrophy calpain-2 activity disrupts muscle fiber integrity through its interaction with proteins like dystrophin aggravating the disorder's progression.
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1 product image
SDS-PAGE - Recombinant Human Calpain 2 protein (His tag) (ab231157)
SDS-PAGE - Recombinant Human Calpain 2 protein (His tag) (ab231157).
(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
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