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AB114576

Recombinant Human Calpain 3 protein

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Recombinant Human Calpain 3 protein is a Human Full Length protein, in the 1 to 309 aa range, expressed in Wheat germ, suitable for SDS-PAGE, ELISA, WB.

View Alternative Names

CANP3, CANPL3, NCL1, CAPN3, Calpain-3, Calcium-activated neutral proteinase 3, Calpain L3, Calpain p94, Muscle-specific calcium-activated neutral protease 3, New calpain 1, CANP 3, nCL-1

1 Images
SDS-PAGE - Recombinant Human Calpain 3 protein (AB114576)
  • SDS-PAGE

Unknown

SDS-PAGE - Recombinant Human Calpain 3 protein (AB114576)

ab114576 on a 12.5% SDS-PAGE Stained with Coomassie Blue.

Key facts

Expression system

Wheat germ

Tags

GST tag N-Terminus

Applications

SDS-PAGE, ELISA, WB

applications

Biologically active

No

Accession

P20807

Animal free

No

Carrier free

No

Species

Human

Storage buffer

pH: 8 Constituents: 0.79% Tris HCl, 0.3% Glutathione

storage-buffer

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "ELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "WB": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p>(Recombinant protein)</p>" } } }
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Sequence info

[{"sequence":"MHGNKQHLQKDFFLYNASKARSKTYINMREVSQRFRLPPSEYVIVPSTYEPHQEGEFILRVFSEKRNLSEEVENTISVDRPVKKKKTKPIIFVSDRANSNKELGVDQESEEGKGKTSPDKQKQSPQPQPGSSDQESEEQQQFRNIFKQIAGDDMEICADELKKVLNTVVNKHKDLKTHGFTLESCRSMIALMDTDGSGKLNLQEFHHLWNKIKAWQKIFKHYDTDQSGTINSYEMRNAVNDAGFHLNNQLYDIITMRYADKHMNIDFDSFICCFVRLEGMFRAFHAFDKDGDGIIKLNVLEWLQLTMYA","proteinLength":"Full Length","predictedMolecularWeight":"59.99 kDa","actualMolecularWeight":null,"aminoAcidEnd":309,"aminoAcidStart":1,"nature":"Recombinant","expressionSystem":"Wheat germ","accessionNumber":"P20807","tags":[{"tag":"GST","terminus":"N-Terminus"}]}]
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Properties and storage information

Shipped at conditions
Dry Ice
Appropriate short-term storage conditions
-80°C
Appropriate long-term storage conditions
-80°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
False
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Calpain 3 also known as p94 or CAPN3 is a calcium-dependent cysteine protease. Its molecular mass is approximately 94 kDa. Calpain 3 is expressed mainly in skeletal muscle tissue. It has a unique structure compared to other calpains possessing specific insertion sequences that are not present in other members of the calpain family. This protein is involved in muscle function through its proteolytic activity where it cleaves target proteins by recognizing specific amino acid sequences.
Biological function summary

Calpain 3 plays an active role in skeletal muscle remodeling and growth. It is a member of the calpain family and works independently not as part of a larger enzyme complex. The protein regulates cytoskeletal remodeling by targeting structural proteins influencing cell motility and muscle integrity. Modifications in Calpain 3 activity can affect muscle elasticity and repair mechanisms which are essential for normal muscle function.

Pathways

Calpain 3 significantly impacts muscle development and maintenance pathways. Its actions in these pathways allow for proper muscle adaptation to stress and changes in physical demands. The protease interacts with titin a large muscle protein and modulates its dynamics influencing how muscle contraction and relaxation occur. Alterations in the signaling pathways involving Calpain 3 and titin can directly affect muscle performance and structural integrity.

Calpain 3 has been directly linked to limb-girdle muscular dystrophy type 2A (LGMD2A). This disorder involves mutations in the CAPN3 gene leading to dysfunctional protein activity. The disease symptoms manifest as progressive weakening of the skeletal muscles. Research also highlights the interaction between Calpain 3 and titin as disruptions to their interaction contribute to the degenerative muscle changes observed in LGMD2A. Scientists continue examining these molecular connections to develop new therapeutic strategies for managing and treating muscle-related disorders.
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Specifications

Form

Liquid

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General info

Function

Calcium-regulated non-lysosomal thiol-protease. Proteolytically cleaves CTBP1 at 'His-409'. Mediates, with UTP25, the proteasome-independent degradation of p53/TP53 (PubMed : 23357851, PubMed : 27657329).

Sequence similarities

Belongs to the peptidase C2 family.

Subcellular localisation

Nucleus

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Product protocols

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Target data

Calcium-regulated non-lysosomal thiol-protease. Proteolytically cleaves CTBP1 at 'His-409'. Mediates, with UTP25, the proteasome-independent degradation of p53/TP53 (PubMed : 23357851, PubMed : 27657329).
See full target information CAPN3
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