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AB128457

Recombinant Human Calponin 1 protein

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Recombinant Human Calponin 1 protein is a Human Full Length protein, in the 1 to 297 aa range, expressed in Escherichia coli, with >90%, suitable for SDS-PAGE, Mass Spec.

View Alternative Names

Calponin-1, Basic calponin, CNN1

1 Images
SDS-PAGE - Recombinant Human Calponin 1 protein (AB128457)
  • SDS-PAGE

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SDS-PAGE - Recombinant Human Calponin 1 protein (AB128457)

15% SDS Page analysis of ab128457 (3ug).

Key facts

Purity

>90% SDS-PAGE

Expression system

Escherichia coli

Tags

His tag C-Terminus

Applications

Mass Spec, SDS-PAGE

applications

Biologically active

No

Accession

P51911

Animal free

No

Carrier free

No

Species

Human

Storage buffer

pH: 8 Constituents: 30% Glycerol (glycerin, glycerine), 0.88% Sodium chloride, 0.32% Tris HCl, 0.02% (R*,R*)-1,4-Dimercaptobutan-2,3-diol

storage-buffer

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "Mass Spec": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Sequence info

[{"sequence":"MSSAHFNRGPAYGLSAEVKNKLAQKYDHQREQELREWIEGVTGRRIGNNFMDGLKDGIILCEFINKLQPGSVKKINESTQNWHQLENIGNFIKAITKYGVKPHDIFEANDLFENTNHTQVQSTLLALASMAKTKGNKVNVGVKYAEKQERKFEPGKLREGRNIIGLQMGTNKFASQQGMTAYGTRRHLYDPKLGTDQPLDQATISLQMGTNKGASQAGMTAPGTKRQIFEPGLGMEHCDTLNVSLQMGSNKGASQRGMTVYGLPRQVYDPKYCLTPEYPELGEPAHNHHAHNYYNSALEHHHHHH","proteinLength":"Full Length","predictedMolecularWeight":"34.2 kDa","actualMolecularWeight":null,"aminoAcidEnd":297,"aminoAcidStart":1,"nature":"Recombinant","expressionSystem":"Escherichia coli","accessionNumber":"P51911","tags":[{"tag":"His","terminus":"C-Terminus"}]}]
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
False
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Calponin 1 also known as CNN1 or calponin-a is a 34-kilodalton protein that plays an important role in muscle contraction. Found mainly in smooth muscle tissues calponin 1 interacts with actin and myosin modulating their interactions and contributing to muscle contraction mechanics. It inhibits the ATPase activity of myosin which affects the contractile capacity of smooth muscle. These interactions are important for maintaining the proper function and tone of vascular and visceral smooth muscles.
Biological function summary

Calponin 1 acts as a regulatory protein in smooth muscle contraction by stabilizing actin filaments and modulating their interaction with other contractile proteins. It forms part of the thin filament-associated complex where it functions to control the conformational state of actin. This complex includes other proteins such as tropomyosin and troponin which together regulate the calcium-sensitive interactions between actin and myosin. The regulatory ability of calponin 1 contributes to the fine-tuning of smooth muscle contraction and relaxation processes.

Pathways

Calponin 1 plays a role in the RhoA/ROCK signaling pathway and the calcium-sensitive contractile pathway. These pathways are integral to smooth muscle contraction regulation and involve proteins such as Rho kinase (ROCK) and myosin light chain kinase (MLCK). Calponin 1 contributes to the modulation of the phosphorylation status of myosin light chains influencing the contractile response to various stimuli. This points to its involvement in the physiological response mechanism to vasoconstrictive agents.

Calponin 1 is linked to cardiovascular diseases like hypertension and vascular disorders. Its regulatory functions in smooth muscle contraction can influence vascular tone and blood pressure connecting it to conditions like atherosclerosis. Moreover calponin 1 interacts with proteins such as filamin and α-actinin which are also implicated in muscle contraction-related disorders. Researchers investigate these interactions to understand their contributions to the pathogenesis of such diseases.
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Specifications

Form

Liquid

Additional notes

ab128457 is purified by using conventional chromatography.

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General info

Function

Thin filament-associated protein that is implicated in the regulation and modulation of smooth muscle contraction. It is capable of binding to actin, calmodulin and tropomyosin. The interaction of calponin with actin inhibits the actomyosin Mg-ATPase activity (By similarity).

Sequence similarities

Belongs to the calponin family.

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Product protocols

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Target data

Thin filament-associated protein that is implicated in the regulation and modulation of smooth muscle contraction. It is capable of binding to actin, calmodulin and tropomyosin. The interaction of calponin with actin inhibits the actomyosin Mg-ATPase activity (By similarity).
See full target information CNN1
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Primary Antibodies

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