Recombinant Human Calreticulin protein is a Human Full Length protein, expressed in Escherichia coli, with >80% purity and suitable for SDS-PAGE, WB.
Images
Publications
Filter by
- BioMed research international 2015:8952842015Calreticulin Binds to Fas Ligand and Inhibits Neuronal Cell Apoptosis Induced by Ischemia-Reperfusion Injury.Applications:Unspecified applicationReactive species:Unspecified reactive speciesBeilei Chen et. al.PubMed 26583143
- American journal of physiology. Heart and circulatory physiology 306:H764-702014MMP-2 is localized to the mitochondria-associated membrane of the heart.Applications:Unspecified applicationReactive species:Unspecified reactive speciesBryan G Hughes,Xiaohu Fan,Woo Jung Cho,Richard SchulzPubMed 24375642
Key facts
- Purity
- >80% SDS-PAGE
- Expression system
- Escherichia coli
- Tags
- His tag N-Terminus
- Applications
- SDS-PAGE, WB
- Biologically active
- No
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application SDS-PAGE | Reactivity Reacts | Dilution info - | Notes - |
Application WB | Reactivity Reacts | Dilution info - | Notes 50 ng of protein recommended. ab91577 can be used as a WB positive control in conjunction with ab39818. |
Associated Products
Select an associated product type
2 products for Alternative Product
Target data
Function
Calcium-binding chaperone that promotes folding, oligomeric assembly and quality control in the endoplasmic reticulum (ER) via the calreticulin/calnexin cycle. This lectin interacts transiently with almost all of the monoglucosylated glycoproteins that are synthesized in the ER (PubMed:7876246). Interacts with the DNA-binding domain of NR3C1 and mediates its nuclear export (PubMed:11149926). Involved in maternal gene expression regulation. May participate in oocyte maturation via the regulation of calcium homeostasis (By similarity). Present in the cortical granules of non-activated oocytes, is exocytosed during the cortical reaction in response to oocyte activation and might participate in the block to polyspermy (By similarity).
Alternative names
CRTC, CALR, Calreticulin, CRP55, Calregulin, Endoplasmic reticulum resident protein 60, HACBP, grp60, ERp60
Recommended products
Recombinant Human Calreticulin protein is a Human Full Length protein, expressed in Escherichia coli, with >80% purity and suitable for SDS-PAGE, WB.
Key facts
- Purity
- >80% SDS-PAGE
- Expression system
- Escherichia coli
- Tags
- His tag N-Terminus
- Applications
- SDS-PAGE, WB
- Biologically active
- No
- Accession
- P27797-1
- Animal free
- No
- Species
- Human
- Concentration
- Storage buffer
Constituents: 5% Glycerol (glycerin, glycerine), 0.877% Sodium chloride, 0.606% Tris, 0.056% Calcium chloride
Sequence info
Amino acid sequence
- Accession
- P27797
- Protein length
- Full Length
- Nature
- Recombinant
- Tags
- His tag N-Terminus
Specifications
- Form
- Liquid
- Additional notes
Purity: > 80 % full-length protein as determined by Western blot analyses
General info
- Function
Calcium-binding chaperone that promotes folding, oligomeric assembly and quality control in the endoplasmic reticulum (ER) via the calreticulin/calnexin cycle. This lectin interacts transiently with almost all of the monoglucosylated glycoproteins that are synthesized in the ER (PubMed:7876246). Interacts with the DNA-binding domain of NR3C1 and mediates its nuclear export (PubMed:11149926). Involved in maternal gene expression regulation. May participate in oocyte maturation via the regulation of calcium homeostasis (By similarity). Present in the cortical granules of non-activated oocytes, is exocytosed during the cortical reaction in response to oocyte activation and might participate in the block to polyspermy (By similarity).
- Sequence similarities
Belongs to the calreticulin family.
- Subcellular localisation
- Cytolytic granule
Storage
- Shipped at conditions
- Dry Ice
- Appropriate short-term storage conditions
- -80°C
- Appropriate long-term storage conditions
- -80°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Product promise
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
3 product images
SDS-PAGE - Recombinant Human Calreticulin protein (ab91577)
SDS-PAGE Analysis of ab91577 stained with Coomassie Blue.
Lane 1: MW Marker
Lane 2: 0.5 μg purified Calreticulin
Lane 3: 1 μg purified Calreticulin
Lane 4: 2 μg purified Calreticulin
Lane 5: 5 μg purified Calreticulin
Western blot - Recombinant Human Calreticulin protein (ab91577)
Lane 1: MW Marker
Lanes 1 - 3: Western blot - Recombinant Human Calreticulin protein (ab91577) at 1/1000 dilution
Lane 2: 50ng Calreticulin
Lane 3: 100ng Calreticulin
Western blot - Recombinant Human Calreticulin protein (ab91577)
All lanes: Anti-Calreticulin antibody - ER Marker (ab39818) at 1 µg/mL
All lanes: Western blot - Recombinant Human Calreticulin protein (ab91577) at 0.01 µg
SecondaryAll lanes: Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) (ab65484) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Exposure time: 30s
Downloads
Product protocols
- Visit the general protocols
- Visit troubleshooting
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
For licensing inquiries, please contact partnerships@abcam.com