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AB91577

Recombinant Human Calreticulin protein

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(2 Publications)

Recombinant Human Calreticulin protein is a Human Full Length protein, expressed in Escherichia coli, with >80%, suitable for SDS-PAGE, WB.

View Alternative Names

CRTC, CALR, Calreticulin, CRP55, Calregulin, Endoplasmic reticulum resident protein 60, HACBP, grp60, ERp60

3 Images
Western blot - Recombinant Human Calreticulin protein (AB91577)
  • WB

Unknown

Western blot - Recombinant Human Calreticulin protein (AB91577)

Lane 1:

MW Marker

Lanes 1 - 3:

Western blot - Recombinant Human Calreticulin protein (ab91577) at 1/1000 dilution

Lane 2:

50ng Calreticulin

Lane 3:

100ng Calreticulin

false

Western blot - Recombinant Human Calreticulin protein (AB91577)
  • WB

Unknown

Western blot - Recombinant Human Calreticulin protein (AB91577)

All lanes:

Anti-Calreticulin antibody - ER Marker (<a href='/en-us/products/unavailable/calreticulin-antibody-er-marker-ab39818'>ab39818</a>) at 1 µg/mL

All lanes:

Western blot - Recombinant Human Calreticulin protein (ab91577) at 0.01 µg

Secondary

All lanes:

Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) (ab65484) at 1/5000 dilution

true

Exposure time: 30s

SDS-PAGE - Recombinant Human Calreticulin protein (AB91577)
  • SDS-PAGE

Unknown

SDS-PAGE - Recombinant Human Calreticulin protein (AB91577)

SDS-PAGE Analysis of ab91577 stained with Coomassie Blue.
Lane 1 : MW Marker
Lane 2 : 0.5 μg purified Calreticulin
Lane 3 : 1 μg purified Calreticulin
Lane 4 : 2 μg purified Calreticulin
Lane 5 : 5 μg purified Calreticulin

Key facts

Purity

>80% SDS-PAGE

Expression system

Escherichia coli

Tags

His tag N-Terminus

Applications

SDS-PAGE, WB

applications

Biologically active

No

Accession

P27797

Animal free

No

Carrier free

No

Species

Human

Storage buffer

Constituents: 5% Glycerol (glycerin, glycerine), 0.877% Sodium chloride, 0.606% Tris, 0.056% Calcium chloride

storage-buffer

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "WB": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p>50 ng of protein recommended. ab91577 can be used as a WB positive control in conjunction with <a href='/en-us/products/unavailable/calreticulin-antibody-er-marker-ab39818'>ab39818</a>.</p>" } } }
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Sequence info

[{"sequence":"","proteinLength":"Full Length","predictedMolecularWeight":null,"actualMolecularWeight":null,"aminoAcidEnd":0,"aminoAcidStart":0,"nature":"Recombinant","expressionSystem":null,"accessionNumber":"P27797","tags":[{"tag":"His","terminus":"N-Terminus"}]}]
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Properties and storage information

Shipped at conditions
Dry Ice
Appropriate short-term storage conditions
-80°C
Appropriate long-term storage conditions
-80°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
False
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Specifications

Form

Liquid

Additional notes

Purity: > 80 % full-length protein as determined by Western blot analyses

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General info

Function

Calcium-binding chaperone that promotes folding, oligomeric assembly and quality control in the endoplasmic reticulum (ER) via the calreticulin/calnexin cycle. This lectin interacts transiently with almost all of the monoglucosylated glycoproteins that are synthesized in the ER (PubMed : 7876246). Interacts with the DNA-binding domain of NR3C1 and mediates its nuclear export (PubMed : 11149926). Involved in maternal gene expression regulation. May participate in oocyte maturation via the regulation of calcium homeostasis (By similarity). Present in the cortical granules of non-activated oocytes, is exocytosed during the cortical reaction in response to oocyte activation and might participate in the block to polyspermy (By similarity).

Sequence similarities

Belongs to the calreticulin family.

Subcellular localisation

Cytolytic granule

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Product protocols

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Target data

Calcium-binding chaperone that promotes folding, oligomeric assembly and quality control in the endoplasmic reticulum (ER) via the calreticulin/calnexin cycle. This lectin interacts transiently with almost all of the monoglucosylated glycoproteins that are synthesized in the ER (PubMed : 7876246). Interacts with the DNA-binding domain of NR3C1 and mediates its nuclear export (PubMed : 11149926). Involved in maternal gene expression regulation. May participate in oocyte maturation via the regulation of calcium homeostasis (By similarity). Present in the cortical granules of non-activated oocytes, is exocytosed during the cortical reaction in response to oocyte activation and might participate in the block to polyspermy (By similarity).
See full target information CALR
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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

BioMed research international 2015:895284 PubMed26583143

2015

Calreticulin Binds to Fas Ligand and Inhibits Neuronal Cell Apoptosis Induced by Ischemia-Reperfusion Injury.

Applications

Unspecified application

Species

Unspecified reactive species

Beilei Chen,Zhengzheng Wu,Jun Xu,Yun Xu

American journal of physiology. Heart and circulatory physiology 306:H764-70 PubMed24375642

2014

MMP-2 is localized to the mitochondria-associated membrane of the heart.

Applications

Unspecified application

Species

Unspecified reactive species

Bryan G Hughes,Xiaohu Fan,Woo Jung Cho,Richard Schulz
View all publications
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