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Recombinant human CaMKII alpha protein is a Human Full Length protein, expressed in Baculovirus infected Sf9, with >80% purity and suitable for SDS-PAGE, WB, FuncS.

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Purity

>80% Densitometry

Expression system

Baculovirus infected Sf9 cells

Tags

GST tag N-Terminus

Applications

SDS-PAGE, WB, FuncS

Biologically active

Yes

Sequence

M S P I L G Y W K I K G L V Q P T R L L L E Y L E E K Y E E H L Y E R D E G D K W R N K K F E L G L E F P N L P Y Y I D G D V K L T Q S M A I I R Y I A D K H N M L G G C P K E R A E I S M L E G A V L D I R Y G V S R I A Y S K D F E T L K V D F L S K L P E M L K M F E D R L C H K T Y L N G D H V T H P D F M L Y D A L D V V L Y M D P M C L D A F P K L V C F K K R I E A I P Q I D K Y L K S S K Y I A W P L Q G W Q A T F G G G D H P P K S D L V P R G S P E F M A T I T C T R F T E E Y Q L F E E L G K G A F S V V R R C V K V L A G Q E Y A A K I I N T K K L S A R D H Q K L E R E A R I C R L L K H P N I V R L H D S I S E E G H H Y L I F D L V T G G E L F E D I V A R E Y Y S E A D A S H C I Q Q I L E A V L H C H Q M G V V H R D L K P E N L L L A S K L K G A A V K L A D F G L A I E V E G E Q Q A W F G F A G T P G Y L S P E V L R K D P Y G K P V D L W A C G V I L Y I L L V G Y P P F W D E D Q H R L Y Q Q I K A G A Y D F P S P E W D T V T P E A K D L I N K M L T I N P S K R I T A A E A L K H P W I S H R S T V A S C M H R Q E T V D C L K K F N A R R K L K G A I L T T M L A T R N F S G G K S G G N K K S D G V K E S S E S T N T T I E D E D T K V R K Q E I I K V T E Q L I E A I S N G D F E S Y T K M C D P G M T A F E P E A L G N L V E G L D F H R F Y F E N L W S R N S K P V H T T I L N P H I H L M G D E S A C I A Y I R I T Q Y L D A G G I P R T A Q S E E T R V W H R R D G K W Q I V H F H R S G A P S V L P H

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Shipping To:
United States

Reactivity data

Application
SDS-PAGE
Reactivity
Reacts
Dilution info
-
Notes

-

Application
WB
Reactivity
Reacts
Dilution info
-
Notes
Ab60899 can be used as a WB positive control in conjunction with ab75930.
Application
FuncS
Reactivity
Reacts
Dilution info
-
Notes
Specific Activity: 932 nmol/min/mg.

Images

Target data

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Recombinant human CaMKII alpha protein is a Human Full Length protein, expressed in Baculovirus infected Sf9, with >80% purity and suitable for SDS-PAGE, WB, FuncS.

Key facts

Purity

>80% Densitometry

Expression system

Baculovirus infected Sf9 cells

Applications

SDS-PAGE, WB, FuncS

Biological activity

256 nmol/min/mg

Predicted molecular weight

74 kDa

Sequence

M S P I L G Y W K I K G L V Q P T R L L L E Y L E E K Y E E H L Y E R D E G D K W R N K K F E L G L E F P N L P Y Y I D G D V K L T Q S M A I I R Y I A D K H N M L G G C P K E R A E I S M L E G A V L D I R Y G V S R I A Y S K D F E T L K V D F L S K L P E M L K M F E D R L C H K T Y L N G D H V T H P D F M L Y D A L D V V L Y M D P M C L D A F P K L V C F K K R I E A I P Q I D K Y L K S S K Y I A W P L Q G W Q A T F G G G D H P P K S D L V P R G S P E F M A T I T C T R F T E E Y Q L F E E L G K G A F S V V R R C V K V L A G Q E Y A A K I I N T K K L S A R D H Q K L E R E A R I C R L L K H P N I V R L H D S I S E E G H H Y L I F D L V T G G E L F E D I V A R E Y Y S E A D A S H C I Q Q I L E A V L H C H Q M G V V H R D L K P E N L L L A S K L K G A A V K L A D F G L A I E V E G E Q Q A W F G F A G T P G Y L S P E V L R K D P Y G K P V D L W A C G V I L Y I L L V G Y P P F W D E D Q H R L Y Q Q I K A G A Y D F P S P E W D T V T P E A K D L I N K M L T I N P S K R I T A A E A L K H P W I S H R S T V A S C M H R Q E T V D C L K K F N A R R K L K G A I L T T M L A T R N F S G G K S G G N K K S D G V K E S S E S T N T T I E D E D T K V R K Q E I I K V T E Q L I E A I S N G D F E S Y T K M C D P G M T A F E P E A L G N L V E G L D F H R F Y F E N L W S R N S K P V H T T I L N P H I H L M G D E S A C I A Y I R I T Q Y L D A G G I P R T A Q S E E T R V W H R R D G K W Q I V H F H R S G A P S V L P H

Accession
Q9UQM7-1
Protein length

Full Length

Animal free

No

Nature

Recombinant

Species

Human

Concentration
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Storage buffer

pH: 7.5
Constituents: 25% Glycerol (glycerin, glycerine), 0.87% Sodium chloride, 0.79% Tris HCl, 0.00385% (R*,R*)-1,4-Dimercaptobutan-2,3-diol, 0.0038% EGTA, 0.00292% EDTA, 0.00174% PMSF

Specifications

Form

Liquid

Additional notes

Affinity purified.

General info

Function

Calcium/calmodulin-dependent protein kinase that functions autonomously after Ca(2+)/calmodulin-binding and autophosphorylation, and is involved in synaptic plasticity, neurotransmitter release and long-term potentiation. Member of the NMDAR signaling complex in excitatory synapses, it regulates NMDAR-dependent potentiation of the AMPAR and therefore excitatory synaptic transmission (By similarity). Regulates dendritic spine development (PubMed:28130356). Also regulates the migration of developing neurons (PubMed:29100089). Phosphorylates the transcription factor FOXO3 to activate its transcriptional activity (PubMed:23805378). Acts as a negative regulator of 2-arachidonoylglycerol (2-AG)-mediated synaptic signaling via modulation of DAGLA activity (By similarity).

Sequence similarities

Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. CaMK subfamily.

Post-translational modifications

Autophosphorylation of Thr-286 following activation by Ca(2+)/calmodulin. Phosphorylation of Thr-286 locks the kinase into an activated state.

Storage

Shipped at conditions

Dry Ice

Appropriate long-term storage conditions

-80°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

This product is an active protein and may elicit a biological response in vivo, handle with caution.

Notes

ab188554 (CaMKII peptide) can be utilized as a substrate for assessing Kinase activity

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

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