Recombinant human CaMKII alpha protein
Be the first to review this product! Submit a review
|
(4 Publications)
Recombinant human CaMKII alpha protein is a Human Full Length protein, expressed in Baculovirus infected Sf9 cells, with >80%, suitable for SDS-PAGE, WB, FuncS.
View Alternative Names
CAMKA, KIAA0968, CAMK2A, Calcium/calmodulin-dependent protein kinase type II subunit alpha, CaM kinase II subunit alpha, CaMK-II subunit alpha
- FuncS
Unknown
Functional Studies - Recombinant human CaMKII alpha protein (AB60899)
Sample Kinase Activity Plot.
- FuncS
Unknown
Functional Studies - Recombinant human CaMKII alpha protein (AB60899)
The specific activity of CaMKII alpha (ab60899) was determined to be 295 nmol/min/mg as per activity assay protocol
- WB
Unknown
Western blot - Recombinant human CaMKII alpha protein (AB60899)
All lanes:
Anti-CaMKII alpha antibody (<a href='/en-us/products/unavailable/camkii-alpha-antibody-ab75930'>ab75930</a>) at 1/100 dilution
All lanes:
Western blot - Recombinant human CaMKII alpha protein (ab60899) at 0.1 µg
Secondary
All lanes:
Western blot - Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-preadsorbed-ab97080'>ab97080</a>) at 1/5000 dilution
true
Exposure time: 10s
- SDS-PAGE
Unknown
SDS-PAGE - Recombinant human CaMKII alpha protein (AB60899)
ab60899 on SDS-PAGE, MW ~74 kDa.
- SDS-PAGE
Unknown
SDS-PAGE - Recombinant human CaMKII alpha protein (AB60899)
SDS PAGE analysis of ab60899
Reactivity data
Product details
Sequence info
Properties and storage information
Shipped at conditions
Appropriate short-term storage conditions
Appropriate long-term storage conditions
Aliquoting information
Storage information
Supplementary information
This supplementary information is collated from multiple sources and compiled automatically.
Biological function summary
CaMKII alpha plays an important role in synaptic plasticity a process vital for learning and memory. The protein acts as an important component of the postsynaptic density complex where it interacts with other proteins to regulate synaptic strength. When calcium levels rise CaMKII alpha is activated through phosphorylation altering its conformation and allowing interactions with downstream substrates. This regulatory mechanism highlights its role in modulating neurotransmission synaptic potentiation and memory consolidation.
Pathways
CaMKII alpha functions within the calcium signaling pathway contributing to long-term potentiation (LTP) and long-term depression (LTD). It interacts with proteins such as NMDA receptors to facilitate changes in synaptic strength alongside other kinases. This pathway's activation leads to memory formation and synaptic plasticity in the central nervous system. Additionally CaMKII alpha connects with the MAPK signaling pathway integrating signals from various neurotransmitter systems which help coordinate neuronal response to stimuli.
Specifications
Form
Liquid
Additional notes
Affinity purified.
General info
Function
Calcium/calmodulin-dependent protein kinase that functions autonomously after Ca(2+)/calmodulin-binding and autophosphorylation, and is involved in various processes, such as synaptic plasticity, neurotransmitter release and long-term potentiation (PubMed : 14722083). Member of the NMDAR signaling complex in excitatory synapses, it regulates NMDAR-dependent potentiation of the AMPAR and therefore excitatory synaptic transmission (By similarity). Regulates dendritic spine development (PubMed : 28130356). Also regulates the migration of developing neurons (PubMed : 29100089). Phosphorylates the transcription factor FOXO3 to activate its transcriptional activity (PubMed : 23805378). Phosphorylates the transcription factor ETS1 in response to calcium signaling, thereby decreasing ETS1 affinity for DNA (By similarity). In response to interferon-gamma (IFN-gamma) stimulation, catalyzes phosphorylation of STAT1, stimulating the JAK-STAT signaling pathway (PubMed : 11972023). In response to interferon-beta (IFN-beta) stimulation, stimulates the JAK-STAT signaling pathway (PubMed : 35568036). Acts as a negative regulator of 2-arachidonoylglycerol (2-AG)-mediated synaptic signaling via modulation of DAGLA activity (By similarity).
Sequence similarities
Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. CaMK subfamily.
Post-translational modifications
Autophosphorylation of Thr-286 following activation by Ca(2+)/calmodulin. Phosphorylation of Thr-286 locks the kinase into an activated state.. Palmitoylated. Probably palmitoylated by ZDHHC3 and ZDHHC7.
Target data
Publications (4)
Recent publications for all applications. Explore the full list and refine your search
Cell reports 41:111724 PubMed36450263
2022
Applications
Unspecified application
Species
Unspecified reactive species
EMBO reports 23:e53373 PubMed34994492
2022
Applications
Unspecified application
Species
Unspecified reactive species
Antioxidants (Basel, Switzerland) 7: PubMed30322141
2018
Applications
Unspecified application
Species
Unspecified reactive species
The Journal of biological chemistry 288:26112-20 PubMed23884421
2013
Applications
Unspecified application
Species
Unspecified reactive species
Alternative Products
Complementary Products
Primary Antibodies
AB2900
Anti-EEA1 antibody - Early Endosome Marker
KO Validated|Lab Essentials
- 4
- 39
Product promise
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
For licensing inquiries, please contact partnerships@abcam.com