Recombinant human CaMKII alpha protein is a Human Full Length protein, expressed in Baculovirus infected Sf9, with >80% purity and suitable for SDS-PAGE, WB, FuncS.
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- Cell reports 41:1117242022β-hydroxybutyrate reduces reinstatement of cocaine conditioned place preference through hippocampal CaMKII-α β-hydroxybutyrylation.Applications:Unspecified applicationReactive species:Unspecified reactive speciesHongchun Li,Xuemei Wan,Zhixiang Wu,Yuanyi Zhou,Rong Chen,Wei Xu,Jiamei Zhang,Zhen Yang,Lin Bai,Jie Zhang,Feng Qin,Liang Wang,Yaxing Chen,Linhong Jiang,Yuman He,Xiaojie Wang,Qingfan Wei,Shu Li,Yanping Dai,Yuanyuan Chen,Yonghai Wang,Hongbo Wang,Jingwei Tian,Yinglan Zhao,Xiaobo CenPubMed 36450263
- EMBO reports 23:e533732022Amino acid starvation-induced LDLR trafficking accelerates lipoprotein endocytosis and LDL clearance.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYe Chen,Xiao Wu,Jing Zhang,Guopin Pan,Xiaoyun Wang,Xiaosun Guo,Jianli Wang,Xiaopei Cui,Haiqing Gao,Mei Cheng,Jingwen Yang,Cheng Zhang,Fan JiangPubMed 34994492
- Antioxidants (Basel, Switzerland) 7:2018Oxidation of Methionine 77 in Calmodulin Alters Mouse Growth and Behavior.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMéry Marimoutou et. al.PubMed 30322141
- The Journal of biological chemistry 288:26112-202013Restoration of glutamatergic transmission by dopamine D4 receptors in stressed animals.Applications:Unspecified applicationReactive species:Unspecified reactive speciesEunice Y Yuen et. al.PubMed 23884421
Key facts
- Purity
- >80% Densitometry
- Expression system
- Baculovirus infected Sf9 cells
- Tags
- GST tag N-Terminus
- Applications
- SDS-PAGE, WB, FuncS
- Biologically active
- Yes
Amino acid sequence
M S P I L G Y W K I K G L V Q P T R L L L E Y L E E K Y E E H L Y E R D E G D K W R N K K F E L G L E F P N L P Y Y I D G D V K L T Q S M A I I R Y I A D K H N M L G G C P K E R A E I S M L E G A V L D I R Y G V S R I A Y S K D F E T L K V D F L S K L P E M L K M F E D R L C H K T Y L N G D H V T H P D F M L Y D A L D V V L Y M D P M C L D A F P K L V C F K K R I E A I P Q I D K Y L K S S K Y I A W P L Q G W Q A T F G G G D H P P K S D L V P R G S P E F M A T I T C T R F T E E Y Q L F E E L G K G A F S V V R R C V K V L A G Q E Y A A K I I N T K K L S A R D H Q K L E R E A R I C R L L K H P N I V R L H D S I S E E G H H Y L I F D L V T G G E L F E D I V A R E Y Y S E A D A S H C I Q Q I L E A V L H C H Q M G V V H R D L K P E N L L L A S K L K G A A V K L A D F G L A I E V E G E Q Q A W F G F A G T P G Y L S P E V L R K D P Y G K P V D L W A C G V I L Y I L L V G Y P P F W D E D Q H R L Y Q Q I K A G A Y D F P S P E W D T V T P E A K D L I N K M L T I N P S K R I T A A E A L K H P W I S H R S T V A S C M H R Q E T V D C L K K F N A R R K L K G A I L T T M L A T R N F S G G K S G G N K K S D G V K E S S E S T N T T I E D E D T K V R K Q E I I K V T E Q L I E A I S N G D F E S Y T K M C D P G M T A F E P E A L G N L V E G L D F H R F Y F E N L W S R N S K P V H T T I L N P H I H L M G D E S A C I A Y I R I T Q Y L D A G G I P R T A Q S E E T R V W H R R D G K W Q I V H F H R S G A P S V L P H
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application SDS-PAGE | Reactivity Reacts | Dilution info - | Notes - |
Application WB | Reactivity Reacts | Dilution info - | Notes ab60899 can be used as a WB positive control in conjunction with ab75930. |
Application FuncS | Reactivity Reacts | Dilution info - | Notes Specific Activity: 932 nmol/min/mg. |
Associated Products
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Target data
Function
Calcium/calmodulin-dependent protein kinase that functions autonomously after Ca(2+)/calmodulin-binding and autophosphorylation, and is involved in various processes, such as synaptic plasticity, neurotransmitter release and long-term potentiation (PubMed:14722083). Member of the NMDAR signaling complex in excitatory synapses, it regulates NMDAR-dependent potentiation of the AMPAR and therefore excitatory synaptic transmission (By similarity). Regulates dendritic spine development (PubMed:28130356). Also regulates the migration of developing neurons (PubMed:29100089). Phosphorylates the transcription factor FOXO3 to activate its transcriptional activity (PubMed:23805378). Phosphorylates the transcription factor ETS1 in response to calcium signaling, thereby decreasing ETS1 affinity for DNA (By similarity). In response to interferon-gamma (IFN-gamma) stimulation, catalyzes phosphorylation of STAT1, stimulating the JAK-STAT signaling pathway (PubMed:11972023). In response to interferon-beta (IFN-beta) stimulation, stimulates the JAK-STAT signaling pathway (PubMed:35568036). Acts as a negative regulator of 2-arachidonoylglycerol (2-AG)-mediated synaptic signaling via modulation of DAGLA activity (By similarity).
Alternative names
CAMKA, KIAA0968, CAMK2A, Calcium/calmodulin-dependent protein kinase type II subunit alpha, CaM kinase II subunit alpha, CaMK-II subunit alpha
Recommended products
Recombinant human CaMKII alpha protein is a Human Full Length protein, expressed in Baculovirus infected Sf9, with >80% purity and suitable for SDS-PAGE, WB, FuncS.
Key facts
- Purity
- >80% Densitometry
- Expression system
- Baculovirus infected Sf9 cells
- Tags
- GST tag N-Terminus
- Applications
- SDS-PAGE, WB, FuncS
- Biologically active
- Yes
- Biological activity
- 256 nmol/min/mg
- Accession
- Q9UQM7-1
- Animal free
- No
- Species
- Human
- Concentration
- Storage buffer
pH: 7.5
Constituents: 25% Glycerol (glycerin, glycerine), 0.87% Sodium chloride, 0.79% Tris HCl, 0.00385% (R*,R*)-1,4-Dimercaptobutan-2,3-diol, 0.0038% EGTA, 0.00292% EDTA, 0.00174% PMSF
Sequence info
Amino acid sequence
- M S P I L G Y W K I K G L V Q P T R L L L E Y L E E K Y E E H L Y E R D E G D K W R N K K F E L G L E F P N L P Y Y I D G D V K L T Q S M A I I R Y I A D K H N M L G G C P K E R A E I S M L E G A V L D I R Y G V S R I A Y S K D F E T L K V D F L S K L P E M L K M F E D R L C H K T Y L N G D H V T H P D F M L Y D A L D V V L Y M D P M C L D A F P K L V C F K K R I E A I P Q I D K Y L K S S K Y I A W P L Q G W Q A T F G G G D H P P K S D L V P R G S P E F M A T I T C T R F T E E Y Q L F E E L G K G A F S V V R R C V K V L A G Q E Y A A K I I N T K K L S A R D H Q K L E R E A R I C R L L K H P N I V R L H D S I S E E G H H Y L I F D L V T G G E L F E D I V A R E Y Y S E A D A S H C I Q Q I L E A V L H C H Q M G V V H R D L K P E N L L L A S K L K G A A V K L A D F G L A I E V E G E Q Q A W F G F A G T P G Y L S P E V L R K D P Y G K P V D L W A C G V I L Y I L L V G Y P P F W D E D Q H R L Y Q Q I K A G A Y D F P S P E W D T V T P E A K D L I N K M L T I N P S K R I T A A E A L K H P W I S H R S T V A S C M H R Q E T V D C L K K F N A R R K L K G A I L T T M L A T R N F S G G K S G G N K K S D G V K E S S E S T N T T I E D E D T K V R K Q E I I K V T E Q L I E A I S N G D F E S Y T K M C D P G M T A F E P E A L G N L V E G L D F H R F Y F E N L W S R N S K P V H T T I L N P H I H L M G D E S A C I A Y I R I T Q Y L D A G G I P R T A Q S E E T R V W H R R D G K W Q I V H F H R S G A P S V L P H
- Accession
- Q9UQM7
- Protein length
- Full Length
- Predicted molecular weight
- 74 kDa
- Nature
- Recombinant
- Tags
- GST tag N-Terminus
Specifications
- Form
- Liquid
- Additional notes
Affinity purified.
General info
- Function
Calcium/calmodulin-dependent protein kinase that functions autonomously after Ca(2+)/calmodulin-binding and autophosphorylation, and is involved in various processes, such as synaptic plasticity, neurotransmitter release and long-term potentiation (PubMed:14722083). Member of the NMDAR signaling complex in excitatory synapses, it regulates NMDAR-dependent potentiation of the AMPAR and therefore excitatory synaptic transmission (By similarity). Regulates dendritic spine development (PubMed:28130356). Also regulates the migration of developing neurons (PubMed:29100089). Phosphorylates the transcription factor FOXO3 to activate its transcriptional activity (PubMed:23805378). Phosphorylates the transcription factor ETS1 in response to calcium signaling, thereby decreasing ETS1 affinity for DNA (By similarity). In response to interferon-gamma (IFN-gamma) stimulation, catalyzes phosphorylation of STAT1, stimulating the JAK-STAT signaling pathway (PubMed:11972023). In response to interferon-beta (IFN-beta) stimulation, stimulates the JAK-STAT signaling pathway (PubMed:35568036). Acts as a negative regulator of 2-arachidonoylglycerol (2-AG)-mediated synaptic signaling via modulation of DAGLA activity (By similarity).
- Sequence similarities
Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. CaMK subfamily.
- Post-translational modifications
Autophosphorylation of Thr-286 following activation by Ca(2+)/calmodulin. Phosphorylation of Thr-286 locks the kinase into an activated state.
Storage
- Shipped at conditions
- Dry Ice
- Appropriate short-term storage conditions
- -80°C
- Appropriate long-term storage conditions
- -80°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
This product is an active protein and may elicit a biological response in vivo, handle with caution.
Notes
CaMKII peptide ab188554 (CaMKII peptide) can be utilized as a substrate for assessing Kinase activity
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
CaMKII alpha also known as CAMK2A is an enzyme belonging to the CaMK kinase family. This protein is a calcium/calmodulin-dependent protein kinase and exhibits a molecular weight of approximately 50 to 60 kDa. CaMKII alpha is primarily expressed in the brain specifically in the hippocampus and other regions involved in synaptic activity. Due to its role as a serine/threonine kinase CaMKII alpha is significant in phosphorylating various substrates as part of cellular signaling.
- Biological function summary
CaMKII alpha plays an important role in synaptic plasticity a process vital for learning and memory. The protein acts as an important component of the postsynaptic density complex where it interacts with other proteins to regulate synaptic strength. When calcium levels rise CaMKII alpha is activated through phosphorylation altering its conformation and allowing interactions with downstream substrates. This regulatory mechanism highlights its role in modulating neurotransmission synaptic potentiation and memory consolidation.
- Pathways
CaMKII alpha functions within the calcium signaling pathway contributing to long-term potentiation (LTP) and long-term depression (LTD). It interacts with proteins such as NMDA receptors to facilitate changes in synaptic strength alongside other kinases. This pathway's activation leads to memory formation and synaptic plasticity in the central nervous system. Additionally CaMKII alpha connects with the MAPK signaling pathway integrating signals from various neurotransmitter systems which help coordinate neuronal response to stimuli.
- Associated diseases and disorders
Alterations in CaMKII alpha have been linked to neurological conditions such as Alzheimer's disease and schizophrenia. In Alzheimer's discourse in CaMKII alpha phosphorylation correlates with impaired memory and cognitive function. The protein also interfaces with tau another key protein related to neurofibrillary tangles in Alzheimer's. In schizophrenia abnormalities in CaMKII alpha expression and activity suggest its role in synaptic dysfunction and neurodevelopmental irregularities. Targeting CaMKII alpha may provide therapeutic opportunities for managing these and related neurological disorders.
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Functional Studies - Recombinant human CaMKII alpha protein (ab60899)
The specific activity of CaMKII alpha (ab60899) was determined to be 295 nmol/min/mg as per activity assay protocol
SDS-PAGE - Recombinant human CaMKII alpha protein (ab60899)
SDS PAGE analysis of ab60899
Functional Studies - Recombinant human CaMKII alpha protein (ab60899)
Sample Kinase Activity Plot.
SDS-PAGE - Recombinant human CaMKII alpha protein (ab60899)
ab60899 on SDS-PAGE, MW ~74 kDa.
Western blot - Recombinant human CaMKII alpha protein (ab60899)
All lanes: Anti-CaMKII alpha antibody (ab75930) at 1/100 dilution
All lanes: Western blot - Recombinant human CaMKII alpha protein (ab60899) at 0.1 µg
SecondaryAll lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (Goat Anti-Rabbit IgG H&L (HRP) preadsorbed ab97080) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Exposure time: 10s
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