Recombinant human CaMKII beta protein (Active) is a Human Full Length protein, in the 1 to 503 aa range, expressed in Baculovirus infected Sf9, with >75% purity and suitable for SDS-PAGE, FuncS.
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- Brain pathology (Zurich, Switzerland) 33:e130992022Targeting the interaction of GABA receptors with CaMKII with an interfering peptide restores receptor expression after cerebral ischemia and inhibits progressive neuronal death in mouse brain cells and slices.Applications:Unspecified applicationReactive species:Unspecified reactive speciesKarthik Balakrishnan,Mohammad Hleihil,Musadiq A Bhat,Robert P Ganley,Markus Vaas,Jan Klohs,Hanns Ulrich Zeilhofer,Dietmar BenkePubMed 35698024
Key facts
- Purity
- >75% SDS-PAGE
- Expression system
- Baculovirus infected Sf9 cells
- Tags
- GST tag N-Terminus
- Applications
- SDS-PAGE, FuncS
- Biologically active
- Yes
Amino acid sequence
M A T T V T C T R F T D E Y Q L Y E D I G K G A F S V V R R C V K L C T G H E Y A A K I I N T K K L S A R D H Q K L E R E A R I C R L L K H S N I V R L H D S I S E E G F H Y L V F D L V T G G E L F E D I V A R E Y Y S E A D A S H C I Q Q I L E A V L H C H Q M G V V H R D L K P E N L L L A S K C K G A A V K L A D F G L A I E V Q G D Q Q A W F G F A G T P G Y L S P E V L R K E A Y G K P V D I W A C G V I L Y I L L V G Y P P F W D E D Q H K L Y Q Q I K A G A Y D F P S P E W D T V T P E A K N L I N Q M L T I N P A K R I T A H E A L K H P W V C Q R S T V A S M M H R Q E T V E C L K K F N A R R K L K G A I L T T M L A T R N F S A A K S L L N K K A D G V K P Q T N S T K N S A A A T S P K G T L P P A A L E S S D S A N T T I E D E D A K A R K Q E I I K T T E Q L I E A V N N G D F E A Y A K I C D P G L T S F E P E A L G N L V E G M D F H R F Y F E N L L A K N S K P I H T T I L N P H V H V I G E D A A C I A Y I R L T Q Y I D G Q G R P R T S Q S E E T R V W H R R D G K W Q N V H F H C S G A P V A P L Q
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application SDS-PAGE | Reactivity Reacts | Dilution info - | Notes - |
Application FuncS | Reactivity Reacts | Dilution info - | Notes - |
Target data
Function
Calcium/calmodulin-dependent protein kinase that functions autonomously after Ca(2+)/calmodulin-binding and autophosphorylation, and is involved in dendritic spine and synapse formation, neuronal plasticity and regulation of sarcoplasmic reticulum Ca(2+) transport in skeletal muscle (PubMed:16690701). In neurons, plays an essential structural role in the reorganization of the actin cytoskeleton during plasticity by binding and bundling actin filaments in a kinase-independent manner. This structural function is required for correct targeting of CaMK2A, which acts downstream of NMDAR to promote dendritic spine and synapse formation and maintain synaptic plasticity which enables long-term potentiation (LTP) and hippocampus-dependent learning. In developing hippocampal neurons, promotes arborization of the dendritic tree and in mature neurons, promotes dendritic remodeling. Also regulates the migration of developing neurons (PubMed:29100089). Participates in the modulation of skeletal muscle function in response to exercise (PubMed:16690701). In slow-twitch muscles, is involved in regulation of sarcoplasmic reticulum (SR) Ca(2+) transport and in fast-twitch muscle participates in the control of Ca(2+) release from the SR through phosphorylation of triadin, a ryanodine receptor-coupling factor, and phospholamban (PLN/PLB), an endogenous inhibitor of SERCA2A/ATP2A2. In response to interferon-gamma (IFN-gamma) stimulation, catalyzes phosphorylation of STAT1, stimulating the JAK-STAT signaling pathway (By similarity). Phosphorylates reticulophagy regulator RETREG1 at 'Ser-151' under endoplasmic reticulum stress conditions which enhances RETREG1 oligomerization and its membrane scission and reticulophagy activity (PubMed:31930741).
Alternative names
CAM2, CAMK2, CAMKB, CAMK2B, Calcium/calmodulin-dependent protein kinase type II subunit beta, CaM kinase II subunit beta, CaMK-II subunit beta
Recommended products
Recombinant human CaMKII beta protein (Active) is a Human Full Length protein, in the 1 to 503 aa range, expressed in Baculovirus infected Sf9, with >75% purity and suitable for SDS-PAGE, FuncS.
Key facts
- Purity
- >75% SDS-PAGE
- Expression system
- Baculovirus infected Sf9 cells
- Tags
- GST tag N-Terminus
- Applications
- SDS-PAGE, FuncS
- Biologically active
- Yes
- Biological activity
- The specific activity of ab268377 was 1,700 nmol/min/mg in a kinase assay using autocamtide 2 synthetic peptide as substrate.
- Accession
- Q13554-1
- Animal free
- No
- Species
- Human
- Concentration
- Storage buffer
pH: 7.5
Constituents: 25% Glycerol (glycerin, glycerine), 0.87% Sodium chloride, 0.79% Tris HCl, 0.31% Glutathione, 0.004% (R*,R*)-1,4-Dimercaptobutan-2,3-diol, 0.003% EDTA, 0.002% PMSF
Sequence info
Amino acid sequence
- M A T T V T C T R F T D E Y Q L Y E D I G K G A F S V V R R C V K L C T G H E Y A A K I I N T K K L S A R D H Q K L E R E A R I C R L L K H S N I V R L H D S I S E E G F H Y L V F D L V T G G E L F E D I V A R E Y Y S E A D A S H C I Q Q I L E A V L H C H Q M G V V H R D L K P E N L L L A S K C K G A A V K L A D F G L A I E V Q G D Q Q A W F G F A G T P G Y L S P E V L R K E A Y G K P V D I W A C G V I L Y I L L V G Y P P F W D E D Q H K L Y Q Q I K A G A Y D F P S P E W D T V T P E A K N L I N Q M L T I N P A K R I T A H E A L K H P W V C Q R S T V A S M M H R Q E T V E C L K K F N A R R K L K G A I L T T M L A T R N F S A A K S L L N K K A D G V K P Q T N S T K N S A A A T S P K G T L P P A A L E S S D S A N T T I E D E D A K A R K Q E I I K T T E Q L I E A V N N G D F E A Y A K I C D P G L T S F E P E A L G N L V E G M D F H R F Y F E N L L A K N S K P I H T T I L N P H V H V I G E D A A C I A Y I R L T Q Y I D G Q G R P R T S Q S E E T R V W H R R D G K W Q N V H F H C S G A P V A P L Q
- Accession
- Q13554
- Protein length
- Full Length
- Amino acids
- 1 to 503
- Nature
- Recombinant
- Tags
- GST tag N-Terminus
Specifications
- Form
- Liquid
- Additional notes
Affinity purified.
General info
- Function
Calcium/calmodulin-dependent protein kinase that functions autonomously after Ca(2+)/calmodulin-binding and autophosphorylation, and is involved in dendritic spine and synapse formation, neuronal plasticity and regulation of sarcoplasmic reticulum Ca(2+) transport in skeletal muscle (PubMed:16690701). In neurons, plays an essential structural role in the reorganization of the actin cytoskeleton during plasticity by binding and bundling actin filaments in a kinase-independent manner. This structural function is required for correct targeting of CaMK2A, which acts downstream of NMDAR to promote dendritic spine and synapse formation and maintain synaptic plasticity which enables long-term potentiation (LTP) and hippocampus-dependent learning. In developing hippocampal neurons, promotes arborization of the dendritic tree and in mature neurons, promotes dendritic remodeling. Also regulates the migration of developing neurons (PubMed:29100089). Participates in the modulation of skeletal muscle function in response to exercise (PubMed:16690701). In slow-twitch muscles, is involved in regulation of sarcoplasmic reticulum (SR) Ca(2+) transport and in fast-twitch muscle participates in the control of Ca(2+) release from the SR through phosphorylation of triadin, a ryanodine receptor-coupling factor, and phospholamban (PLN/PLB), an endogenous inhibitor of SERCA2A/ATP2A2. In response to interferon-gamma (IFN-gamma) stimulation, catalyzes phosphorylation of STAT1, stimulating the JAK-STAT signaling pathway (By similarity). Phosphorylates reticulophagy regulator RETREG1 at 'Ser-151' under endoplasmic reticulum stress conditions which enhances RETREG1 oligomerization and its membrane scission and reticulophagy activity (PubMed:31930741).
- Sequence similarities
Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. CaMK subfamily.
- Post-translational modifications
Autophosphorylation of Thr-287 following activation by Ca(2+)/calmodulin. Phosphorylation of Thr-287 locks the kinase into an activated state.
- Subcellular localisation
- Cytoskeleton, Microtubule organizing center, Centrosome
Storage
- Shipped at conditions
- Dry Ice
- Appropriate short-term storage conditions
- -80°C
- Appropriate long-term storage conditions
- -80°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
This product is an active protein and may elicit a biological response in vivo, handle with caution.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
CaMKII beta also called CaM-K 2B is a calcium/calmodulin-dependent protein kinase involved in many cellular processes. It is known for its molecular weight of approximately 73 kDa. Expressed widely in tissues CaMKII beta is particularly important in brain and heart tissues. CaMKII family which includes CaMK plays a role in catalyzing the phosphorylation of serine and threonine residues in proteins influencing varied cellular activities.
- Biological function summary
Without referring directly to CaMKII beta this protein contributes to synaptic plasticity which influences learning and memory in neural cells. It functions as part of a multimeric complex forming a holoenzyme with other CaMKII isoforms like CaMKII alpha gamma and delta. The structural dynamics of its autophosphorylation regulate its prolonged activity independent of calcium and calmodulin affecting downstream signaling.
- Pathways
Without directly mentioning CaMKII beta it is essential in the Ca2+/calmodulin signaling pathway critical for regulating neuronal activity. Additionally this kinase participates in the cardiac conduction system influencing the heartbeat. Related proteins like calcineurin and protein kinase A interact in these signaling pathways amplifying the role of CaMKII beta in translating calcium bursts into longer-term cellular responses.
- Associated diseases and disorders
Without direct mention CaMKII beta's involvement is evident in conditions such as Alzheimer's disease and arrhythmias. In neurodegenerative diseases its deregulation affects the balance of phosphorylation involving proteins like tau which contribute to disease progression. In cardiac disorders its role in arrhythmias interacts with ion channels affecting heart rhythm stability. Understanding these interactions helps in therapeutic approaches targeting CaMKII beta-related pathways.
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2 product images
Functional Studies - Recombinant human CaMKII beta protein (Active) (ab268377)
The specific activity of ab268377 was 1,700 nmol/min/mg in a kinase assay using autocamtide 2 synthetic peptide as substrate.
SDS-PAGE - Recombinant human CaMKII beta protein (Active) (ab268377)
SDS-PAGE analysis of ab268377.
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