Recombinant Human CANT1 protein is a Human Fragment protein, in the 63 to 401 aa range, expressed in Escherichia coli, with >90% purity and suitable for SDS-PAGE, MS.
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Key facts
- Purity
- >90% SDS-PAGE
- Expression system
- Escherichia coli
- Tags
- His tag N-Terminus
- Applications
- SDS-PAGE, MS
- Biologically active
- No
Amino acid sequence
M G S S H H H H H H S S G L V P R G S H M G S H M R P A P G R P P T H N A H N W R L G Q A P A N W Y N D T Y P L S P P Q R T P A G I R Y R I A V I A D L D T E S R A Q E E N T W F S Y L K K G Y L T L S D S G D K V A V E W D K D H G V L E S H L A E K G R G M E L S D L I V F N G K L Y S V D D R T G V V Y Q I E G S K A V P W V I L S D G D G T V E K G F K A E W L A V K D E R L Y V G G L G K E W T T T T G D V V N E N P E W V K V V G Y K G S V D H E N W V S N Y N A L R A A A G I Q P P G Y L I H E S A C W S D T L Q R W F F L P R R A S Q E R Y S E K D D E R K G A N L L L S A S P D F G D I A V S H V G A V V P T H G F S S F K F I P N T D D Q I I V A L K S E E D S G R V A S Y I M A F T L D G R F L L P E T K I G S V K Y E G I E F I
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application SDS-PAGE | Reactivity Reacts | Dilution info - | Notes - |
Application MS | Reactivity Reacts | Dilution info - | Notes - |
Target data
Function
Calcium-dependent nucleotidase with a preference for UDP. The order of activity with different substrates is UDP > GDP > UTP > GTP. Has very low activity towards ADP and even lower activity towards ATP. Does not hydrolyze AMP and GMP (PubMed:12234496, PubMed:15006348, PubMed:15248776, PubMed:16835225). Involved in proteoglycan synthesis (PubMed:22539336).
Alternative names
SHAPY, CANT1, Soluble calcium-activated nucleotidase 1, SCAN-1, Apyrase homolog, Putative MAPK-activating protein PM09, Putative NF-kappa-B-activating protein 107
Recommended products
Recombinant Human CANT1 protein is a Human Fragment protein, in the 63 to 401 aa range, expressed in Escherichia coli, with >90% purity and suitable for SDS-PAGE, MS.
Key facts
- Purity
- >90% SDS-PAGE
- Expression system
- Escherichia coli
- Tags
- His tag N-Terminus
- Applications
- SDS-PAGE, MS
- Biologically active
- No
- Accession
- Q8WVQ1-1
- Animal free
- No
- Species
- Human
- Concentration
- Storage buffer
pH: 8
Constituents: 10% Glycerol (glycerin, glycerine), 0.32% Tris HCl, 0.29% Sodium chloride, 0.02% (R*,R*)-1,4-Dimercaptobutan-2,3-diol
Sequence info
Amino acid sequence
- M G S S H H H H H H S S G L V P R G S H M G S H M R P A P G R P P T H N A H N W R L G Q A P A N W Y N D T Y P L S P P Q R T P A G I R Y R I A V I A D L D T E S R A Q E E N T W F S Y L K K G Y L T L S D S G D K V A V E W D K D H G V L E S H L A E K G R G M E L S D L I V F N G K L Y S V D D R T G V V Y Q I E G S K A V P W V I L S D G D G T V E K G F K A E W L A V K D E R L Y V G G L G K E W T T T T G D V V N E N P E W V K V V G Y K G S V D H E N W V S N Y N A L R A A A G I Q P P G Y L I H E S A C W S D T L Q R W F F L P R R A S Q E R Y S E K D D E R K G A N L L L S A S P D F G D I A V S H V G A V V P T H G F S S F K F I P N T D D Q I I V A L K S E E D S G R V A S Y I M A F T L D G R F L L P E T K I G S V K Y E G I E F I
- Accession
- Q8WVQ1
- Protein length
- Fragment
- Predicted molecular weight
- 40.5 kDa
- Amino acids
- 63 to 401
- Nature
- Recombinant
- Tags
- His tag N-Terminus
Specifications
- Form
- Liquid
- Additional notes
ab123189 is purified using conventional chromatography techniques.
General info
- Function
Calcium-dependent nucleotidase with a preference for UDP. The order of activity with different substrates is UDP > GDP > UTP > GTP. Has very low activity towards ADP and even lower activity towards ATP. Does not hydrolyze AMP and GMP (PubMed:12234496, PubMed:15006348, PubMed:15248776, PubMed:16835225). Involved in proteoglycan synthesis (PubMed:22539336).
- Sequence similarities
Belongs to the apyrase family.
- Post-translational modifications
N-glycosylated.
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- -20°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
CANT1 also known as calcium-activated nucleotidase 1 acts as a nucleotidase enzyme that removes phosphate groups from nucleotides. It catalyzes the hydrolysis of UDP ADP and GDP to their respective monophosphates. The protein of CANT1 has an approximate molecular mass of 41 kDa. This protein is widely distributed throughout human tissues with higher expression levels observed in skeletal muscle and heart.
- Biological function summary
CANT1 influences cellular processes by regulating extracellular nucleotide signaling. It involves in protein glycosylation a process critical for protein folding and stability. CANT1 does not form part of a complex but functions independently to exert its biological effects. Glycosylation impacts how proteins function and signal across the body reflecting the importance of CANT1's enzymatic role in various cells.
- Pathways
Multiple cellular pathways depend on CANT1’s enzymatic activity. The protein is notably engaged in the purinergic signaling pathway where it modulates extracellular nucleotide levels that influence signaling cascades. CANT1 shares functional relationships with proteins such as ENTPD5 and NTPDases which partake in similar nucleotide hydrolysis processes. It also has connections to metabolic processes within glucose metabolism.
- Associated diseases and disorders
Aberrations in CANT1's function link to diseases like Desbuquois dysplasia and specific forms of osteochondrodysplasia. In these conditions defects in glycosylation arise due to CANT1 mutations and affect bone growth and development. This protein also interacts with proteins involved in bone disorders including SLC35D1 contributing to the pathology observed in affected patients.
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1 product image
SDS-PAGE - Recombinant Human CANT1 protein (ab123189)
15% SDS-PAGE showing ab123189 (3μg).
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