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AB171494

Recombinant Human CAPZA2 protein (denatured) (His tag N-Terminus)

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Recombinant Human CAPZA2 protein (denatured) (His tag N-Terminus) is a Human Full Length protein, in the 1 to 286 aa range, expressed in Escherichia coli, with >85%, suitable for SDS-PAGE.

View Alternative Names

F-actin-capping protein subunit alpha-2, CapZ alpha-2, CAPZA2

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SDS-PAGE - Recombinant Human CAPZA2 protein (denatured) (His tag N-Terminus) (AB171494)
  • SDS-PAGE

Unknown

SDS-PAGE - Recombinant Human CAPZA2 protein (denatured) (His tag N-Terminus) (AB171494)

15% SDS-PAGE analysis of 3µg ab171494.

Key facts

Purity

>85% SDS-PAGE

Expression system

Escherichia coli

Tags

His tag N-Terminus

Applications

SDS-PAGE

applications

Biologically active

No

Accession

P47755

Animal free

No

Carrier free

No

Species

Human

Storage buffer

pH: 8 Constituents: 10% Glycerol (glycerin, glycerine), 2.4% Urea, 0.32% Tris HCl

storage-buffer

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Sequence info

[{"sequence":"MGSSHHHHHHSSGLVPRGSHMGSMADLEEQLSDEEKVRIAAKFIIHAPPGEFNEVFNDVRLLLNNDNLLREGAAHAFAQYNLDQFTPVKIEGYEDQVLITEHGDLGNGKFLDPKNRICFKFDHLRKEATDPRPCEVENAVESWRTSVETALRAYVKEHYPNGVCTVYGKKIDGQQTIIACIESHQFQAKNFWNGRWRSEWKFTITPSTTQVVGILKIQVHYYEDGNVQLVSHKDIQDSLTVSNEVQTAKEFIKIVEAAENEYQTAISENYQTMSDTTFKALRRQLPVTRTKIDWNKILSYKIGKEMQNA","proteinLength":"Full Length","predictedMolecularWeight":"35.4 kDa","actualMolecularWeight":null,"aminoAcidEnd":286,"aminoAcidStart":1,"nature":"Recombinant","expressionSystem":"Escherichia coli","accessionNumber":"P47755","tags":[{"tag":"His","terminus":"N-Terminus"}]}]
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
False
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

CAPZA2 also known as capping protein (actin filament) muscle Z-line alpha 2 is a protein that mechanically functions to regulate actin filament dynamics by binding to the barbed ends. This protein has an approximate molecular mass of 33 kDa. CAPZA2 is predominantly expressed in muscle tissues suggesting its significant role in muscle function. The protein forms part of the actin capping protein complex specifically interacting with the CAPZB subunit.
Biological function summary

CAPZA2 controls the growth of actin filaments an essential process for cell motility and stability. It is part of the capping protein complex which is necessary for the capping and stabilization of the fast-growing ends of actin filaments. This stabilization prevents further polymerization maintaining optimal filament length for cellular dynamics. CAPZA2 also plays a role in cytoskeletal organization important for muscle contraction and cell shape maintenance.

Pathways

The actin-regulating activity of CAPZA2 integrates into pathways involving actin cytoskeleton organization and dynamics. It is significantly involved in the actin-mediated processes interacting with proteins such as Arp2/3 complex which initiates actin branching. CAPZA2's role in cytoskeleton assembly also positions it within the signaling pathways related to cell growth and muscle function impacting interactions with proteins like tropomyosin which stabilizes actin filaments.

CAPZA2 shows a connection to muscular disorders including myopathy. Dysfunction in actin filament regulation can lead to issues in muscle fiber integrity and function contributing to muscle weakness or degenerative conditions. CAPZA2 is also linked to cardiac disorders due to its important role in muscle contractility interacting with proteins like myosin which is vital for heart muscle function. Understanding CAPZA2's roles can aid in developing therapeutic targets for muscle-related diseases.
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Specifications

Form

Liquid

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General info

Function

F-actin-capping proteins bind in a Ca(2+)-independent manner to the fast growing ends of actin filaments (barbed end) thereby blocking the exchange of subunits at these ends. Unlike other capping proteins (such as gelsolin and severin), these proteins do not sever actin filaments.

Sequence similarities

Belongs to the F-actin-capping protein alpha subunit family.

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Product protocols

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Target data

F-actin-capping proteins bind in a Ca(2+)-independent manner to the fast growing ends of actin filaments (barbed end) thereby blocking the exchange of subunits at these ends. Unlike other capping proteins (such as gelsolin and severin), these proteins do not sever actin filaments.
See full target information F-actin-capping protein subunit alpha-2
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Complementary Products

Primary Antibodies

AB175378

Anti-CAPZA2 antibody

Immunocytochemistry/ Immunofluorescence - Anti-CAPZA2 antibody (AB175378)

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  • 0

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