Recombinant Human CAPZA2 protein (denatured) is a Human Full Length protein, in the 1 to 286 aa range, expressed in Escherichia coli, with >85% purity and suitable for SDS-PAGE.
Images
Key facts
- Purity
- >85% SDS-PAGE
- Expression system
- Escherichia coli
- Tags
- His tag N-Terminus
- Applications
- SDS-PAGE
- Biologically active
- No
Amino acid sequence
M G S S H H H H H H S S G L V P R G S H M G S M A D L E E Q L S D E E K V R I A A K F I I H A P P G E F N E V F N D V R L L L N N D N L L R E G A A H A F A Q Y N L D Q F T P V K I E G Y E D Q V L I T E H G D L G N G K F L D P K N R I C F K F D H L R K E A T D P R P C E V E N A V E S W R T S V E T A L R A Y V K E H Y P N G V C T V Y G K K I D G Q Q T I I A C I E S H Q F Q A K N F W N G R W R S E W K F T I T P S T T Q V V G I L K I Q V H Y Y E D G N V Q L V S H K D I Q D S L T V S N E V Q T A K E F I K I V E A A E N E Y Q T A I S E N Y Q T M S D T T F K A L R R Q L P V T R T K I D W N K I L S Y K I G K E M Q N A
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application SDS-PAGE | Reactivity Reacts | Dilution info - | Notes - |
Target data
Function
F-actin-capping proteins bind in a Ca(2+)-independent manner to the fast growing ends of actin filaments (barbed end) thereby blocking the exchange of subunits at these ends. Unlike other capping proteins (such as gelsolin and severin), these proteins do not sever actin filaments.
Alternative names
F-actin-capping protein subunit alpha-2, CapZ alpha-2, CAPZA2
Recommended products
Recombinant Human CAPZA2 protein (denatured) is a Human Full Length protein, in the 1 to 286 aa range, expressed in Escherichia coli, with >85% purity and suitable for SDS-PAGE.
Key facts
- Purity
- >85% SDS-PAGE
- Expression system
- Escherichia coli
- Tags
- His tag N-Terminus
- Applications
- SDS-PAGE
- Biologically active
- No
- Accession
- P47755-1
- Animal free
- No
- Species
- Human
- Concentration
- Storage buffer
pH: 8
Constituents: 10% Glycerol (glycerin, glycerine), 2.4% Urea, 0.32% Tris HCl
Sequence info
Amino acid sequence
- M G S S H H H H H H S S G L V P R G S H M G S M A D L E E Q L S D E E K V R I A A K F I I H A P P G E F N E V F N D V R L L L N N D N L L R E G A A H A F A Q Y N L D Q F T P V K I E G Y E D Q V L I T E H G D L G N G K F L D P K N R I C F K F D H L R K E A T D P R P C E V E N A V E S W R T S V E T A L R A Y V K E H Y P N G V C T V Y G K K I D G Q Q T I I A C I E S H Q F Q A K N F W N G R W R S E W K F T I T P S T T Q V V G I L K I Q V H Y Y E D G N V Q L V S H K D I Q D S L T V S N E V Q T A K E F I K I V E A A E N E Y Q T A I S E N Y Q T M S D T T F K A L R R Q L P V T R T K I D W N K I L S Y K I G K E M Q N A
- Accession
- P47755
- Protein length
- Full Length
- Predicted molecular weight
- 35.4 kDa
- Amino acids
- 1 to 286
- Nature
- Recombinant
- Tags
- His tag N-Terminus
Specifications
- Form
- Liquid
General info
- Function
F-actin-capping proteins bind in a Ca(2+)-independent manner to the fast growing ends of actin filaments (barbed end) thereby blocking the exchange of subunits at these ends. Unlike other capping proteins (such as gelsolin and severin), these proteins do not sever actin filaments.
- Sequence similarities
Belongs to the F-actin-capping protein alpha subunit family.
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
CAPZA2 also known as capping protein (actin filament) muscle Z-line alpha 2 is a protein that mechanically functions to regulate actin filament dynamics by binding to the barbed ends. This protein has an approximate molecular mass of 33 kDa. CAPZA2 is predominantly expressed in muscle tissues suggesting its significant role in muscle function. The protein forms part of the actin capping protein complex specifically interacting with the CAPZB subunit.
- Biological function summary
CAPZA2 controls the growth of actin filaments an essential process for cell motility and stability. It is part of the capping protein complex which is necessary for the capping and stabilization of the fast-growing ends of actin filaments. This stabilization prevents further polymerization maintaining optimal filament length for cellular dynamics. CAPZA2 also plays a role in cytoskeletal organization important for muscle contraction and cell shape maintenance.
- Pathways
The actin-regulating activity of CAPZA2 integrates into pathways involving actin cytoskeleton organization and dynamics. It is significantly involved in the actin-mediated processes interacting with proteins such as Arp2/3 complex which initiates actin branching. CAPZA2's role in cytoskeleton assembly also positions it within the signaling pathways related to cell growth and muscle function impacting interactions with proteins like tropomyosin which stabilizes actin filaments.
- Associated diseases and disorders
CAPZA2 shows a connection to muscular disorders including myopathy. Dysfunction in actin filament regulation can lead to issues in muscle fiber integrity and function contributing to muscle weakness or degenerative conditions. CAPZA2 is also linked to cardiac disorders due to its important role in muscle contractility interacting with proteins like myosin which is vital for heart muscle function. Understanding CAPZA2's roles can aid in developing therapeutic targets for muscle-related diseases.
Product promise
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
1 product image
SDS-PAGE - Recombinant Human CAPZA2 protein (denatured) (ab171494)
15% SDS-PAGE analysis of 3µg ab171494.
Downloads
Product protocols
- Visit the general protocols
- Visit troubleshooting
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
For licensing inquiries, please contact partnerships@abcam.com