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AB89359

Recombinant Human Carbonic Anhydrase 1/CA1 protein (His tag N-Terminus)

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Recombinant Human Carbonic Anhydrase 1/CA1 protein (His tag N-Terminus) is a Human Full Length protein, expressed in Escherichia coli, with >95%, suitable for SDS-PAGE, WB.

View Alternative Names

Carbonic anhydrase 1, Carbonate dehydratase I, Carbonic anhydrase B, Carbonic anhydrase I, Cyanamide hydratase CA1, CAB, CA-I, CA1

2 Images
Western blot - Recombinant Human Carbonic Anhydrase 1/CA1 protein (His tag N-Terminus) (AB89359)
  • WB

Unknown

Western blot - Recombinant Human Carbonic Anhydrase 1/CA1 protein (His tag N-Terminus) (AB89359)

All lanes:

Anti-Carbonic Anhydrase I antibody (<a href='/en-us/products/unavailable/carbonic-anhydrase-1ca1-antibody-ab92649'>ab92649</a>) at 1/500 dilution

All lanes:

Western blot - Recombinant Human Carbonic Anhydrase 1/CA1 protein (His tag N-Terminus) (ab89359) at 0.01 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-preadsorbed-ab97080'>ab97080</a>) at 1/5000 dilution

true

Exposure time: 20s

SDS-PAGE - Recombinant Human Carbonic Anhydrase 1/CA1 protein (His tag N-Terminus) (AB89359)
  • SDS-PAGE

Supplier Data

SDS-PAGE - Recombinant Human Carbonic Anhydrase 1/CA1 protein (His tag N-Terminus) (AB89359)

SDS-PAGE analysis of ab89359 (3 μg) under reducing conditions and visualized by coomassie blue stain.

Key facts

Purity

>95% SDS-PAGE

Expression system

Escherichia coli

Tags

His tag N-Terminus

Applications

WB, SDS-PAGE

applications

Biologically active

No

Accession

P00915

Animal free

No

Carrier free

No

Species

Human

Storage buffer

pH: 8 Constituents: 10% Glycerol (glycerin, glycerine), 0.316% Tris HCl, 0.0154% (R*,R*)-1,4-Dimercaptobutan-2,3-diol

storage-buffer

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Complementary Products

Secondary Antibodies

AB150113

Goat Anti-Mouse IgG H&L (Alexa Fluor® 488)

Immunocytochemistry/ Immunofluorescence - Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) (AB150113)

  • 5
  • 15
Proteins & Peptides

AB87361

Recombinant Human 14-3-3 zeta protein (His tag N-Terminus)

SDS-PAGE - Recombinant Human 14-3-3 zeta protein (AB87361)

  • 0
  • 0
Primary Antibodies

AB267475

Anti-Carbonic Anhydrase 1/CA1 antibody [EPR23232-286]

BOND RX™ Validated|RabMAb|Recombinant

Flow Cytometry (Intracellular) - Anti-Carbonic Anhydrase 1/CA1 antibody [EPR23232-286] (AB267475)

  • 0
  • 0
Biochemicals

AB120573

L-DOPA, Dopamine (ab120565) precursor

Chemical Structure - L-DOPA, Dopamine (ab120565) precursor (AB120573)

  • 0
  • 0
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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "WB": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p>ab89359 can be used as a WB positive control in conjunction with <a href='/en-us/products/unavailable/carbonic-anhydrase-1ca1-antibody-ab92649'>ab92649</a>.</p>" } } }
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Product details

This product was previously labelled as Carbonic Anhydrase I
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Sequence info

[{"sequence":"","proteinLength":"Full Length","predictedMolecularWeight":null,"actualMolecularWeight":null,"aminoAcidEnd":0,"aminoAcidStart":0,"nature":"Recombinant","expressionSystem":null,"accessionNumber":"P00915","tags":[{"tag":"His","terminus":"N-Terminus"}]}]
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
False
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Specifications

Form

Liquid

Additional notes

ab89359 is purified using conventional chromatography techniques.

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General info

Function

Catalyzes the reversible hydration of carbon dioxide (PubMed : 10550681, PubMed : 16506782, PubMed : 16686544, PubMed : 16807956, PubMed : 17127057, PubMed : 17314045, PubMed : 17407288, PubMed : 18618712, PubMed : 19186056, PubMed : 19206230). Can hydrate cyanamide to urea (PubMed : 10550681).

Sequence similarities

Belongs to the alpha-carbonic anhydrase family.

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Product protocols

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Target data

Catalyzes the reversible hydration of carbon dioxide (PubMed : 10550681, PubMed : 16506782, PubMed : 16686544, PubMed : 16807956, PubMed : 17127057, PubMed : 17314045, PubMed : 17407288, PubMed : 18618712, PubMed : 19186056, PubMed : 19206230). Can hydrate cyanamide to urea (PubMed : 10550681).
See full target information Carbonic anhydrase 1
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Product promise

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