Recombinant Human Carbonic anhydrase 2/CA2 protein is a Human Full Length protein, expressed in Escherichia coli, with >95% purity and suitable for SDS-PAGE, FuncS.
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- Genomics, proteomics & bioinformatics 19:94-1072021Quantitative Secretome Analysis Reveals Clinical Values of Carbonic Anhydrase II in Hepatocellular Carcinoma.Applications:Unspecified applicationReactive species:Unspecified reactive speciesXiaohua Xing,Hui Yuan,Hongzhi Liu,Xionghong Tan,Bixing Zhao,Yingchao Wang,Jiahe Ouyang,Minjie Lin,Xiaolong Liu,Aimin HuangPubMed 33662630
Key facts
- Purity
- >95% SDS-PAGE
- Expression system
- Escherichia coli
- Tags
- Tag free
- Applications
- SDS-PAGE, FuncS
- Biologically active
- Yes
Amino acid sequence
M S H H W G Y G K H N G P E H W H K D F P I A K G E R Q S P V D I D T H T A K Y D P S L K P L S V S Y D Q A T S L R I L N N G H A F N V E F D D S Q D K A V L K G G P L D G T Y R L I Q F H F H W G S L D G Q G S E H T V D K K K Y A A E L H L V H W N T K Y G D F G K A V Q Q P D G L A V L G I F L K V G S A K P G L Q K V V D V L D S I K T K G K S A D F T N F D P R G L L P E S L D Y W T Y P G S L T T P P L L E C V T W I V L K E P I S V S S E Q V L K F R K L N F N G E G E P E E L M V D N W R P A Q P L K N R Q I K A S F K
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application SDS-PAGE | Reactivity Reacts | Dilution info - | Notes - |
Application FuncS | Reactivity Reacts | Dilution info - | Notes - |
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Target data
Function
Catalyzes the reversible hydration of carbon dioxide (PubMed:11327835, PubMed:11802772, PubMed:11831900, PubMed:12056894, PubMed:12171926, PubMed:1336460, PubMed:14736236, PubMed:15300855, PubMed:15453828, PubMed:15667203, PubMed:15865431, PubMed:16106378, PubMed:16214338, PubMed:16290146, PubMed:16686544, PubMed:16759856, PubMed:16807956, PubMed:17127057, PubMed:17251017, PubMed:17314045, PubMed:17330962, PubMed:17346964, PubMed:17540563, PubMed:17588751, PubMed:17705204, PubMed:18024029, PubMed:18162396, PubMed:18266323, PubMed:18374572, PubMed:18481843, PubMed:18618712, PubMed:18640037, PubMed:18942852, PubMed:1909891, PubMed:1910042, PubMed:19170619, PubMed:19186056, PubMed:19206230, PubMed:19520834, PubMed:19778001, PubMed:7761440, PubMed:7901850, PubMed:8218160, PubMed:8262987, PubMed:8399159, PubMed:8451242, PubMed:8485129, PubMed:8639494, PubMed:9265618, PubMed:9398308). Can also hydrate cyanamide to urea (PubMed:10550681, PubMed:11015219). Stimulates the chloride-bicarbonate exchange activity of SLC26A6 (PubMed:15990874). Essential for bone resorption and osteoclast differentiation (PubMed:15300855). Involved in the regulation of fluid secretion into the anterior chamber of the eye. Contributes to intracellular pH regulation in the duodenal upper villous epithelium during proton-coupled peptide absorption.
Alternative names
Carbonic anhydrase 2, Carbonate dehydratase II, Carbonic anhydrase C, Carbonic anhydrase II, Cyanamide hydratase CA2, CAC, CA-II, CA2
Recommended products
Recombinant Human Carbonic anhydrase 2/CA2 protein is a Human Full Length protein, expressed in Escherichia coli, with >95% purity and suitable for SDS-PAGE, FuncS.
Key facts
- Purity
- >95% SDS-PAGE
- Expression system
- Escherichia coli
- Tags
- Tag free
- Applications
- SDS-PAGE, FuncS
- Biologically active
- Yes
- Biological activity
- Specific activity is > 7,000pmol/min/μg, and is defined as the amount of enzyme that hydrolyze 1.0pmole of 4-nitrophenyl acetate to 4-nitrophenol per minute at pH 8.0 at 37°C.
- Accession
- P00918-1
- Animal free
- No
- Species
- Human
- Concentration
- Storage buffer
pH: 7.4
Constituents: 90% PBS, 10% Glycerol (glycerin, glycerine)
Sequence info
Amino acid sequence
- M S H H W G Y G K H N G P E H W H K D F P I A K G E R Q S P V D I D T H T A K Y D P S L K P L S V S Y D Q A T S L R I L N N G H A F N V E F D D S Q D K A V L K G G P L D G T Y R L I Q F H F H W G S L D G Q G S E H T V D K K K Y A A E L H L V H W N T K Y G D F G K A V Q Q P D G L A V L G I F L K V G S A K P G L Q K V V D V L D S I K T K G K S A D F T N F D P R G L L P E S L D Y W T Y P G S L T T P P L L E C V T W I V L K E P I S V S S E Q V L K F R K L N F N G E G E P E E L M V D N W R P A Q P L K N R Q I K A S F K
- Accession
- P00918
- Protein length
- Full Length
- Nature
- Recombinant
Specifications
- Form
- Liquid
- Additional notes
ab85484 was purified by using conventional chromatography.
General info
- Function
Catalyzes the reversible hydration of carbon dioxide (PubMed:11327835, PubMed:11802772, PubMed:11831900, PubMed:12056894, PubMed:12171926, PubMed:1336460, PubMed:14736236, PubMed:15300855, PubMed:15453828, PubMed:15667203, PubMed:15865431, PubMed:16106378, PubMed:16214338, PubMed:16290146, PubMed:16686544, PubMed:16759856, PubMed:16807956, PubMed:17127057, PubMed:17251017, PubMed:17314045, PubMed:17330962, PubMed:17346964, PubMed:17540563, PubMed:17588751, PubMed:17705204, PubMed:18024029, PubMed:18162396, PubMed:18266323, PubMed:18374572, PubMed:18481843, PubMed:18618712, PubMed:18640037, PubMed:18942852, PubMed:1909891, PubMed:1910042, PubMed:19170619, PubMed:19186056, PubMed:19206230, PubMed:19520834, PubMed:19778001, PubMed:7761440, PubMed:7901850, PubMed:8218160, PubMed:8262987, PubMed:8399159, PubMed:8451242, PubMed:8485129, PubMed:8639494, PubMed:9265618, PubMed:9398308). Can also hydrate cyanamide to urea (PubMed:10550681, PubMed:11015219). Stimulates the chloride-bicarbonate exchange activity of SLC26A6 (PubMed:15990874). Essential for bone resorption and osteoclast differentiation (PubMed:15300855). Involved in the regulation of fluid secretion into the anterior chamber of the eye. Contributes to intracellular pH regulation in the duodenal upper villous epithelium during proton-coupled peptide absorption.
- Sequence similarities
Belongs to the alpha-carbonic anhydrase family.
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- -20°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
This product is an active protein and may elicit a biological response in vivo, handle with caution.
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1 product image
SDS-PAGE - Recombinant Human Carbonic anhydrase 2/CA2 protein (ab85484)
15% SDS-PAGE analysis of 3μg ab85484.
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