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Recombinant Human Carbonic anhydrase 2/CA2 protein is a Human Full Length protein, expressed in Escherichia coli, with >95% purity and suitable for SDS-PAGE, FuncS.

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Key facts

Purity

>95% SDS-PAGE

Expression system

Escherichia coli

Tags

Tag free

Applications

SDS-PAGE, FuncS

Biologically active

Yes

Amino acid sequence

M S H H W G Y G K H N G P E H W H K D F P I A K G E R Q S P V D I D T H T A K Y D P S L K P L S V S Y D Q A T S L R I L N N G H A F N V E F D D S Q D K A V L K G G P L D G T Y R L I Q F H F H W G S L D G Q G S E H T V D K K K Y A A E L H L V H W N T K Y G D F G K A V Q Q P D G L A V L G I F L K V G S A K P G L Q K V V D V L D S I K T K G K S A D F T N F D P R G L L P E S L D Y W T Y P G S L T T P P L L E C V T W I V L K E P I S V S S E Q V L K F R K L N F N G E G E P E E L M V D N W R P A Q P L K N R Q I K A S F K

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Reactivity data

Application

SDS-PAGE

Reactivity

Reacts

Dilution info

-

Notes

-

Application

FuncS

Reactivity

Reacts

Dilution info

-

Notes

-

Target data

Function

Essential for bone resorption and osteoclast differentiation (By similarity). Reversible hydration of carbon dioxide. Can hydrate cyanamide to urea. Involved in the regulation of fluid secretion into the anterior chamber of the eye. Contributes to intracellular pH regulation in the duodenal upper villous epithelium during proton-coupled peptide absorption. Stimulates the chloride-bicarbonate exchange activity of SLC26A6.

Alternative names

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Recombinant Human Carbonic anhydrase 2/CA2 protein is a Human Full Length protein, expressed in Escherichia coli, with >95% purity and suitable for SDS-PAGE, FuncS.

Alternative names

Key facts

Purity

>95% SDS-PAGE

Expression system

Escherichia coli

Applications

SDS-PAGE, FuncS

Biological activity

Specific activity is > 7,000pmol/min/µg, and is defined as the amount of enzyme that hydrolyze 1.0pmole of 4-nitrophenyl acetate to 4-nitrophenol per minute at pH 8.0 at 37°C.

Accession
P00918-1
Animal free

No

Species

Human

Concentration
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Storage buffer

pH: 7.4
Constituents: 90% PBS, 10% Glycerol (glycerin, glycerine)

Sequence info

Amino acid sequence

M S H H W G Y G K H N G P E H W H K D F P I A K G E R Q S P V D I D T H T A K Y D P S L K P L S V S Y D Q A T S L R I L N N G H A F N V E F D D S Q D K A V L K G G P L D G T Y R L I Q F H F H W G S L D G Q G S E H T V D K K K Y A A E L H L V H W N T K Y G D F G K A V Q Q P D G L A V L G I F L K V G S A K P G L Q K V V D V L D S I K T K G K S A D F T N F D P R G L L P E S L D Y W T Y P G S L T T P P L L E C V T W I V L K E P I S V S S E Q V L K F R K L N F N G E G E P E E L M V D N W R P A Q P L K N R Q I K A S F K

Accession

P00918

Protein length

Full Length

Nature

Recombinant

Specifications

Form

Liquid

Additional notes

ab85484 was purified by using conventional chromatography.

General info

Function

Essential for bone resorption and osteoclast differentiation (By similarity). Reversible hydration of carbon dioxide. Can hydrate cyanamide to urea. Involved in the regulation of fluid secretion into the anterior chamber of the eye. Contributes to intracellular pH regulation in the duodenal upper villous epithelium during proton-coupled peptide absorption. Stimulates the chloride-bicarbonate exchange activity of SLC26A6.

Sequence similarities

Belongs to the alpha-carbonic anhydrase family.

Storage

Shipped at conditions

Blue Ice

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

This product is an active protein and may elicit a biological response in vivo, handle with caution.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

1 product image

  • SDS-PAGE - Recombinant Human Carbonic anhydrase 2/CA2 protein (ab85484), expandable thumbnail

    SDS-PAGE - Recombinant Human Carbonic anhydrase 2/CA2 protein (ab85484)

    15% SDS-PAGE analysis of 3μg ab85484.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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