Recombinant Human Carbonic anhydrase 2/CA2 protein (His tag) is a Human Full Length protein, in the 1 to 260 aa range, expressed in HEK 293, with >95% purity, < 1 EU/µg endotoxin level and suitable for SDS-PAGE.
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Key facts
- Purity
>95% SDS-PAGE
- Endotoxin level
< 1 EU/µg
- Expression system
HEK 293 cells
- Tags
His tag C-Terminus
- Applications
SDS-PAGE
- Biologically active
No
Amino acid sequence
M S H H W G Y G K H N G P E H W H K D F P I A K G E R Q S P V D I D T H T A K Y D P S L K P L S V S Y D Q A T S L R I L N N G H A F N V E F D D S Q D K A V L K G G P L D G T Y R L I Q F H F H W G S L D G Q G S E H T V D K K K Y A A E L H L V H W N T K Y G D F G K A V Q Q P D G L A V L G I F L K V G S A K P G L Q K V V D V L D S I K T K G K S A D F T N F D P R G L L P E S L D Y W T Y P G S L T T P P L L E C V T W I V L K E P I S V S S E Q V L K F R K L N F N G E G E P E E L M V D N W R P A Q P L K N R Q I K A S F K
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application SDS-PAGE | Reactivity Reacts | Dilution info - | Notes The protein migrates as 31-33 kDa under reducing condition due to glycosylation. |
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Target data
Function
Essential for bone resorption and osteoclast differentiation (By similarity). Reversible hydration of carbon dioxide. Can hydrate cyanamide to urea. Involved in the regulation of fluid secretion into the anterior chamber of the eye. Contributes to intracellular pH regulation in the duodenal upper villous epithelium during proton-coupled peptide absorption. Stimulates the chloride-bicarbonate exchange activity of SLC26A6.
Alternative names
Carbonic anhydrase 2, Carbonate dehydratase II, Carbonic anhydrase C, Carbonic anhydrase II, CAC, CA-II, CA2
Recommended products
Recombinant Human Carbonic anhydrase 2/CA2 protein (His tag) is a Human Full Length protein, in the 1 to 260 aa range, expressed in HEK 293, with >95% purity, < 1 EU/µg endotoxin level and suitable for SDS-PAGE.
Alternative names
Carbonic anhydrase 2, Carbonate dehydratase II, Carbonic anhydrase C, Carbonic anhydrase II, CAC, CA-II, CA2
Key facts
- Purity
>95% SDS-PAGE
- Endotoxin level
< 1 EU/µg
- Expression system
HEK 293 cells
- Tags
His tag C-Terminus
- Applications
SDS-PAGE
- Biologically active
No
- Accession
- P00918-1
- Animal free
No
- Species
Human
- Reconstitution
It is recommended to reconstitute the lyophilized protein in 500µl sterile deionized water to a final concentration of 0.200 mg/ml. Solubilize for 30 to 60 minutes at room temperature with occasional gentle mixing. Carrier protein (0.1% HSA or BSA) is strongly recommended for further dilution and long term storage.
- Concentration
- Storage buffer
pH: 7.4
Constituents: 0.88% Sodium chloride, 0.24% Tris
Sequence info
Amino acid sequence
M S H H W G Y G K H N G P E H W H K D F P I A K G E R Q S P V D I D T H T A K Y D P S L K P L S V S Y D Q A T S L R I L N N G H A F N V E F D D S Q D K A V L K G G P L D G T Y R L I Q F H F H W G S L D G Q G S E H T V D K K K Y A A E L H L V H W N T K Y G D F G K A V Q Q P D G L A V L G I F L K V G S A K P G L Q K V V D V L D S I K T K G K S A D F T N F D P R G L L P E S L D Y W T Y P G S L T T P P L L E C V T W I V L K E P I S V S S E Q V L K F R K L N F N G E G E P E E L M V D N W R P A Q P L K N R Q I K A S F K
- Accession
- Protein length
Full Length
- Predicted molecular weight
30.1 kDa
- Amino acids
1 to 260
- Nature
Recombinant
- Tags
His tag C-Terminus
Specifications
- Form
Lyophilized
- Additional notes
Purified by Immobilized metal affinity chromatography.
General info
- Function
Essential for bone resorption and osteoclast differentiation (By similarity). Reversible hydration of carbon dioxide. Can hydrate cyanamide to urea. Involved in the regulation of fluid secretion into the anterior chamber of the eye. Contributes to intracellular pH regulation in the duodenal upper villous epithelium during proton-coupled peptide absorption. Stimulates the chloride-bicarbonate exchange activity of SLC26A6.
- Sequence similarities
Belongs to the alpha-carbonic anhydrase family.
Storage
- Shipped at conditions
Blue Ice
- Appropriate long-term storage conditions
-20°C
- Aliquoting information
Upon delivery aliquot
- Storage information
Avoid freeze / thaw cycle
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1 product image
SDS-PAGE - Recombinant Human Carbonic anhydrase 2/CA2 protein (His tag) (ab167716)
SDS-PAGE of reduced ab167716 stained overnight with Coomassie Blue. The protein migrates as 31-33 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
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