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AB167716

Recombinant Human Carbonic anhydrase 2/CA2 protein (His tag)

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Recombinant Human Carbonic anhydrase 2/CA2 protein (His tag) is a Human Full Length protein, in the 1 to 260 aa range, expressed in HEK 293 cells, with >95%, < 1 EU/µg endotoxin level, suitable for SDS-PAGE.

View Alternative Names

Carbonic anhydrase 2, Carbonate dehydratase II, Carbonic anhydrase C, Carbonic anhydrase II, Cyanamide hydratase CA2, CAC, CA-II, CA2

1 Images
SDS-PAGE - Recombinant Human Carbonic anhydrase 2/CA2 protein (His tag) (AB167716)
  • SDS-PAGE

Unknown

SDS-PAGE - Recombinant Human Carbonic anhydrase 2/CA2 protein (His tag) (AB167716)

SDS-PAGE of reduced ab167716 stained overnight with Coomassie Blue. The protein migrates as 31-33 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

Key facts

Purity

>95% SDS-PAGE

Endotoxin level

< 1 EU/µg

Expression system

HEK 293 cells

Tags

His tag C-Terminus

Applications

SDS-PAGE

applications

Biologically active

No

Accession

P00918

Animal free

No

Carrier free

No

Species

Human

Reconstitution

Reconstitute in 500 µL of water

Storage buffer

pH: 7.4 Constituents: 0.88% Sodium chloride, 0.24% Tris

storage-buffer

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Complementary Products

Reagents, Controls & Accessories

AB133411

Low Fluorescence Western Membrane (PVDF)

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Secondary Antibodies

AB150077

Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488)

Immunocytochemistry/ Immunofluorescence - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (AB150077)

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Primary Antibodies

AB124687

Anti-Carbonic anhydrase 2/CA2 antibody [EPR5195]

BOND RX™ Validated|RabMAb|Recombinant|KO Validated

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Carbonic anhydrase 2/CA2 antibody [EPR5195] (AB124687)

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Assay Kits

AB222881

Human Carbonic Anhydrase 2 ELISA Kit

Sandwich ELISA - Human Carbonic Anhydrase 2 ELISA Kit (AB222881)

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Assay Kits

AB283387

Carbonic Anhydrase (CA) Inhibitor Screening Kit (Colorimetric)

Functional Studies - Carbonic Anhydrase (CA) Inhibitor Screening Kit (Colorimetric) (AB283387)

  • 0
  • 0
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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p>The protein migrates as 31-33 kDa under reducing condition due to glycosylation.</p>" } } }
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Sequence info

[{"sequence":"MSHHWGYGKHNGPEHWHKDFPIAKGERQSPVDIDTHTAKYDPSLKPLSVSYDQATSLRILNNGHAFNVEFDDSQDKAVLKGGPLDGTYRLIQFHFHWGSLDGQGSEHTVDKKKYAAELHLVHWNTKYGDFGKAVQQPDGLAVLGIFLKVGSAKPGLQKVVDVLDSIKTKGKSADFTNFDPRGLLPESLDYWTYPGSLTTPPLLECVTWIVLKEPISVSSEQVLKFRKLNFNGEGEPEELMVDNWRPAQPLKNRQIKASFK","proteinLength":"Full Length","predictedMolecularWeight":"30.1 kDa","actualMolecularWeight":null,"aminoAcidEnd":260,"aminoAcidStart":1,"nature":"Recombinant","expressionSystem":"HEK 293 cells","accessionNumber":"P00918","tags":[{"tag":"His","terminus":"C-Terminus"}]}]
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
False
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Specifications

Form

Lyophilized

Additional notes

Purified by Immobilized metal affinity chromatography.

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General info

Function

Catalyzes the reversible hydration of carbon dioxide (PubMed : 11327835, PubMed : 11802772, PubMed : 11831900, PubMed : 12056894, PubMed : 12171926, PubMed : 1336460, PubMed : 14736236, PubMed : 15300855, PubMed : 15453828, PubMed : 15667203, PubMed : 15865431, PubMed : 16106378, PubMed : 16214338, PubMed : 16290146, PubMed : 16686544, PubMed : 16759856, PubMed : 16807956, PubMed : 17127057, PubMed : 17251017, PubMed : 17314045, PubMed : 17330962, PubMed : 17346964, PubMed : 17540563, PubMed : 17588751, PubMed : 17705204, PubMed : 18024029, PubMed : 18162396, PubMed : 18266323, PubMed : 18374572, PubMed : 18481843, PubMed : 18618712, PubMed : 18640037, PubMed : 18942852, PubMed : 1909891, PubMed : 1910042, PubMed : 19170619, PubMed : 19186056, PubMed : 19206230, PubMed : 19520834, PubMed : 19778001, PubMed : 7761440, PubMed : 7901850, PubMed : 8218160, PubMed : 8262987, PubMed : 8399159, PubMed : 8451242, PubMed : 8485129, PubMed : 8639494, PubMed : 9265618, PubMed : 9398308). Can also hydrate cyanamide to urea (PubMed : 10550681, PubMed : 11015219). Stimulates the chloride-bicarbonate exchange activity of SLC26A6 (PubMed : 15990874). Essential for bone resorption and osteoclast differentiation (PubMed : 15300855). Involved in the regulation of fluid secretion into the anterior chamber of the eye. Contributes to intracellular pH regulation in the duodenal upper villous epithelium during proton-coupled peptide absorption.

Sequence similarities

Belongs to the alpha-carbonic anhydrase family.

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Product protocols

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Target data

Catalyzes the reversible hydration of carbon dioxide (PubMed : 11327835, PubMed : 11802772, PubMed : 11831900, PubMed : 12056894, PubMed : 12171926, PubMed : 1336460, PubMed : 14736236, PubMed : 15300855, PubMed : 15453828, PubMed : 15667203, PubMed : 15865431, PubMed : 16106378, PubMed : 16214338, PubMed : 16290146, PubMed : 16686544, PubMed : 16759856, PubMed : 16807956, PubMed : 17127057, PubMed : 17251017, PubMed : 17314045, PubMed : 17330962, PubMed : 17346964, PubMed : 17540563, PubMed : 17588751, PubMed : 17705204, PubMed : 18024029, PubMed : 18162396, PubMed : 18266323, PubMed : 18374572, PubMed : 18481843, PubMed : 18618712, PubMed : 18640037, PubMed : 18942852, PubMed : 1909891, PubMed : 1910042, PubMed : 19170619, PubMed : 19186056, PubMed : 19206230, PubMed : 19520834, PubMed : 19778001, PubMed : 7761440, PubMed : 7901850, PubMed : 8218160, PubMed : 8262987, PubMed : 8399159, PubMed : 8451242, PubMed : 8485129, PubMed : 8639494, PubMed : 9265618, PubMed : 9398308). Can also hydrate cyanamide to urea (PubMed : 10550681, PubMed : 11015219). Stimulates the chloride-bicarbonate exchange activity of SLC26A6 (PubMed : 15990874). Essential for bone resorption and osteoclast differentiation (PubMed : 15300855). Involved in the regulation of fluid secretion into the anterior chamber of the eye. Contributes to intracellular pH regulation in the duodenal upper villous epithelium during proton-coupled peptide absorption.
See full target information CA2
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