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AB198056

Recombinant human Carbonic Anhydrase 9/CA9 protein (Active)

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Recombinant human Carbonic Anhydrase 9/CA9 protein (Active) is a Human Fragment protein, in the 59 to 414 aa range, expressed in Baculovirus infected Sf9 cells, with >65%, suitable for SDS-PAGE, FuncS.

View Alternative Names

G250, MN, CA9, Carbonic anhydrase 9, Carbonate dehydratase IX, Carbonic anhydrase IX, Membrane antigen MN, P54/58N, Renal cell carcinoma-associated antigen G250, pMW1, CA-IX, CAIX, RCC-associated antigen G250

2 Images
Functional Studies - Recombinant human Carbonic Anhydrase 9/CA9 protein (Active) (AB198056)
  • FuncS

Supplier Data

Functional Studies - Recombinant human Carbonic Anhydrase 9/CA9 protein (Active) (AB198056)

Specific activity of ab198056 was determined to be 30,000 pmol/min/μg

SDS-PAGE - Recombinant human Carbonic Anhydrase 9/CA9 protein (Active) (AB198056)
  • SDS-PAGE

Supplier Data

SDS-PAGE - Recombinant human Carbonic Anhydrase 9/CA9 protein (Active) (AB198056)

4-20% SDS-PAGE analysis of 3.8 μg ab198056 with Coomassie staining.

Key facts

Purity

>65% SDS-PAGE

Expression system

Baculovirus infected Sf9 cells

Tags

His tag C-Terminus

Applications

SDS-PAGE, FuncS

applications

Biologically active

Yes

Biological activity

Specific Activity: ≥ 30,000 pmol/min/μg

Assay conditions: 15 mM Tris-HCl, pH7.4, 100mM NaCl, and 3mM p-Nitrophenyl acetate (PNPA). Incubate for 30 minutes at room temperature. Absorbance is measured at 405nM.

Accession

Q16790

Animal free

No

Carrier free

No

Species

Human

Storage buffer

pH: 8 Preservative: 1.81% Imidazole Constituents: 20% Glycerol (glycerin, glycerine), 0.64% Sodium chloride, 0.63% Tris HCl, 0.04% Sorbitan monolaurate, ethoxylated, 0.02% Potassium chloride

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "FuncS": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Sequence info

[{"sequence":"MPLGEEDLPSEEDSPREEDPPGEEDLPGEEDLPGEEDLPEVKPKSEEEGSLKLEDLPTVEAPGDPQEPQNNAHRDKEGDDQSHWRYGGDPPWPRVSPACAGRFQSPVDIRPQLAAFCPALRPLELLGFQLPPLPELRLRNNGHSVQLTLPPGLEMALGPGREYRALQLHLHWGAAGRPGSEHTVEGHRFPAEIHVVHLSTAFARVDEALGRPGGLAVLAAFLEEGPEENSAYEQLLSRLEEIAEEGSETQVPGLDISALLPSDFSRYFQYEGSLTTPPCAQGVIWTVFNQTVMLSAKQLHTLSDTLWGPGDSRLQLNFRATQPLNGRVIEASFPAGVDSSPRAAEPVQLNSCLAAGDHHHHHH","proteinLength":"Fragment","predictedMolecularWeight":"39.7 kDa","actualMolecularWeight":null,"aminoAcidEnd":414,"aminoAcidStart":59,"nature":"Recombinant","expressionSystem":"Baculovirus infected Sf9 cells","accessionNumber":"Q16790","tags":[{"tag":"His","terminus":"C-Terminus"}]}]
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Properties and storage information

Shipped at conditions
Dry Ice
Appropriate short-term storage conditions
-80°C
Appropriate long-term storage conditions
-80°C
Storage information
Avoid freeze / thaw cycle
True
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Specifications

Form

Liquid

Additional notes

Affinity purified.

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General info

Function

Catalyzes the interconversion between carbon dioxide and water and the dissociated ions of carbonic acid (i.e. bicarbonate and hydrogen ions).

Sequence similarities

Belongs to the alpha-carbonic anhydrase family.

Post-translational modifications

Asn-346 bears high-mannose type glycan structures.

Subcellular localisation

Nucleus

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Product protocols

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Target data

Catalyzes the interconversion between carbon dioxide and water and the dissociated ions of carbonic acid (i.e. bicarbonate and hydrogen ions).
See full target information CA9
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