Recombinant human Carbonic Anhydrase 9/CA9 protein (Active) is a Human Fragment protein, in the 59 to 414 aa range, expressed in Baculovirus infected Sf9, with >=65% purity and suitable for SDS-PAGE, FuncS.
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Key facts
- Purity
- >=65% SDS-PAGE
- Expression system
- Baculovirus infected Sf9 cells
- Tags
- His tag C-Terminus
- Applications
- SDS-PAGE, FuncS
- Biologically active
- Yes
Amino acid sequence
M P L G E E D L P S E E D S P R E E D P P G E E D L P G E E D L P G E E D L P E V K P K S E E E G S L K L E D L P T V E A P G D P Q E P Q N N A H R D K E G D D Q S H W R Y G G D P P W P R V S P A C A G R F Q S P V D I R P Q L A A F C P A L R P L E L L G F Q L P P L P E L R L R N N G H S V Q L T L P P G L E M A L G P G R E Y R A L Q L H L H W G A A G R P G S E H T V E G H R F P A E I H V V H L S T A F A R V D E A L G R P G G L A V L A A F L E E G P E E N S A Y E Q L L S R L E E I A E E G S E T Q V P G L D I S A L L P S D F S R Y F Q Y E G S L T T P P C A Q G V I W T V F N Q T V M L S A K Q L H T L S D T L W G P G D S R L Q L N F R A T Q P L N G R V I E A S F P A G V D S S P R A A E P V Q L N S C L A A G D H H H H H H
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application SDS-PAGE | Reactivity Reacts | Dilution info - | Notes - |
Application FuncS | Reactivity Reacts | Dilution info - | Notes - |
Associated Products
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Target data
Function
Catalyzes the interconversion between carbon dioxide and water and the dissociated ions of carbonic acid (i.e. bicarbonate and hydrogen ions).
Alternative names
G250, MN, CA9, Carbonic anhydrase 9, Carbonate dehydratase IX, Carbonic anhydrase IX, Membrane antigen MN, P54/58N, Renal cell carcinoma-associated antigen G250, pMW1, CA-IX, CAIX, RCC-associated antigen G250
Recommended products
Recombinant human Carbonic Anhydrase 9/CA9 protein (Active) is a Human Fragment protein, in the 59 to 414 aa range, expressed in Baculovirus infected Sf9, with >=65% purity and suitable for SDS-PAGE, FuncS.
Key facts
- Purity
- >=65% SDS-PAGE
- Expression system
- Baculovirus infected Sf9 cells
- Tags
- His tag C-Terminus
- Applications
- SDS-PAGE, FuncS
- Biologically active
- Yes
- Biological activity
- Specific Activity: ≥ 30,000 pmol/min/μg
Assay conditions: 15 mM Tris-HCl, pH7.4, 100mM NaCl, and 3mM p-Nitrophenyl acetate (PNPA). Incubate for 30 minutes at room temperature. Absorbance is measured at 405nM.
- Accession
- Q16790-1
- Animal free
- No
- Species
- Human
- Concentration
- Storage buffer
pH: 8
Preservative: 1.81% Imidazole
Constituents: 20% Glycerol (glycerin, glycerine), 0.64% Sodium chloride, 0.63% Tris HCl, 0.04% Sorbitan monolaurate, ethoxylated, 0.02% Potassium chloride
Sequence info
Amino acid sequence
- M P L G E E D L P S E E D S P R E E D P P G E E D L P G E E D L P G E E D L P E V K P K S E E E G S L K L E D L P T V E A P G D P Q E P Q N N A H R D K E G D D Q S H W R Y G G D P P W P R V S P A C A G R F Q S P V D I R P Q L A A F C P A L R P L E L L G F Q L P P L P E L R L R N N G H S V Q L T L P P G L E M A L G P G R E Y R A L Q L H L H W G A A G R P G S E H T V E G H R F P A E I H V V H L S T A F A R V D E A L G R P G G L A V L A A F L E E G P E E N S A Y E Q L L S R L E E I A E E G S E T Q V P G L D I S A L L P S D F S R Y F Q Y E G S L T T P P C A Q G V I W T V F N Q T V M L S A K Q L H T L S D T L W G P G D S R L Q L N F R A T Q P L N G R V I E A S F P A G V D S S P R A A E P V Q L N S C L A A G D H H H H H H
- Accession
- Q16790
- Protein length
- Fragment
- Predicted molecular weight
- 39.7 kDa
- Amino acids
- 59 to 414
- Nature
- Recombinant
- Tags
- His tag C-Terminus
Specifications
- Form
- Liquid
- Additional notes
Affinity purified.
General info
- Function
Catalyzes the interconversion between carbon dioxide and water and the dissociated ions of carbonic acid (i.e. bicarbonate and hydrogen ions).
- Sequence similarities
Belongs to the alpha-carbonic anhydrase family.
- Post-translational modifications
Asn-346 bears high-mannose type glycan structures.
- Subcellular localisation
- Nucleus, Nucleolus
Storage
- Shipped at conditions
- Dry Ice
- Appropriate short-term storage conditions
- -80°C
- Appropriate long-term storage conditions
- -80°C
- Storage information
- Avoid freeze / thaw cycle
This product is an active protein and may elicit a biological response in vivo, handle with caution.
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2 product images
Functional Studies - Recombinant human Carbonic Anhydrase 9/CA9 protein (Active) (ab198056)
Specific activity of ab198056 was determined to be 30,000 pmol/min/μg
SDS-PAGE - Recombinant human Carbonic Anhydrase 9/CA9 protein (Active) (ab198056)
4-20% SDS-PAGE analysis of 3.8 μg ab198056 with Coomassie staining.
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