Recombinant Human Carbonic Anhydrase 9 / CA9 Protein (Active) is a Human Fragment protein, in the 59 to 414 aa range, expressed in HEK 293, with >=95% purity, <= 0.005 EU/µg endotoxin level and suitable for HPLC, MS, SDS-PAGE.
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Key facts
- Purity
- >=95% HPLC
- Endotoxin level
- <= 0.005 EU/µg
- Expression system
- HEK 293 cells
- Tags
- Tag free
- Applications
- HPLC, MS, SDS-PAGE
- Biologically active
- Yes
Amino acid sequence
P L G E E D L P S E E D S P R E E D P P G E E D L P G E E D L P G E E D L P E V K P K S E E E G S L K L E D L P T V E A P G D P Q E P Q N N A H R D K E G D D Q S H W R Y G G D P P W P R V S P A C A G R F Q S P V D I R P Q L A A F C P A L R P L E L L G F Q L P P L P E L R L R N N G H S V Q L T L P P G L E M A L G P G R E Y R A L Q L H L H W G A A G R P G S E H T V E G H R F P A E I H V V H L S T A F A R V D E A L G R P G G L A V L A A F L E E G P E E N S A Y E Q L L S R L E E I A E E G S E T Q V P G L D I S A L L P S D F S R Y F Q Y E G S L T T P P C A Q G V I W T V F N Q T V M L S A K Q L H T L S D T L W G P G D S R L Q L N F R A T Q P L N G R V I E A S F P A G V D S S P R A A E P V Q L N S C L A A G D
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application HPLC | Reactivity Reacts | Dilution info - | Notes - |
Application MS | Reactivity Reacts | Dilution info - | Notes - |
Application SDS-PAGE | Reactivity Reacts | Dilution info - | Notes - |
Target data
Function
Catalyzes the interconversion between carbon dioxide and water and the dissociated ions of carbonic acid (i.e. bicarbonate and hydrogen ions).
Alternative names
G250, MN, CA9, Carbonic anhydrase 9, Carbonate dehydratase IX, Carbonic anhydrase IX, Membrane antigen MN, P54/58N, Renal cell carcinoma-associated antigen G250, pMW1, CA-IX, CAIX, RCC-associated antigen G250
Recommended products
Recombinant Human Carbonic Anhydrase 9 / CA9 Protein (Active) is a Human Fragment protein, in the 59 to 414 aa range, expressed in HEK 293, with >=95% purity, <= 0.005 EU/µg endotoxin level and suitable for HPLC, MS, SDS-PAGE.
Key facts
- Purity
- >=95% HPLC
- Endotoxin level
- <= 0.005 EU/µg
- Expression system
- HEK 293 cells
- Tags
- Tag free
- Applications
- HPLC, MS, SDS-PAGE
- Biologically active
- Yes
- Biological activity
- Fully active measured by its esterase activity. The specific activity is ≥ 200 pmol/min/ug.
- Accession
- Q16790-1
- Animal free
- Yes
- Species
- Human
- Concentration
- Storage buffer
pH: 7.4
Constituents: 10.26% Trehalose, 0.727% Dibasic monohydrogen potassium phosphate, 0.248% Potassium phosphate monobasic
Sequence info
Amino acid sequence
- P L G E E D L P S E E D S P R E E D P P G E E D L P G E E D L P G E E D L P E V K P K S E E E G S L K L E D L P T V E A P G D P Q E P Q N N A H R D K E G D D Q S H W R Y G G D P P W P R V S P A C A G R F Q S P V D I R P Q L A A F C P A L R P L E L L G F Q L P P L P E L R L R N N G H S V Q L T L P P G L E M A L G P G R E Y R A L Q L H L H W G A A G R P G S E H T V E G H R F P A E I H V V H L S T A F A R V D E A L G R P G G L A V L A A F L E E G P E E N S A Y E Q L L S R L E E I A E E G S E T Q V P G L D I S A L L P S D F S R Y F Q Y E G S L T T P P C A Q G V I W T V F N Q T V M L S A K Q L H T L S D T L W G P G D S R L Q L N F R A T Q P L N G R V I E A S F P A G V D S S P R A A E P V Q L N S C L A A G D
- Accession
- Q16790
- Protein length
- Fragment
- Predicted molecular weight
- 38.81 kDa
- Actual molecular weight
- 38.81 kDa
- Amino acids
- 59 to 414
- Nature
- Recombinant
Specifications
- Form
- Liquid
- Additional notes
SDS-PAGE >= 95%
General info
- Function
Catalyzes the interconversion between carbon dioxide and water and the dissociated ions of carbonic acid (i.e. bicarbonate and hydrogen ions).
- Sequence similarities
Belongs to the alpha-carbonic anhydrase family.
- Post-translational modifications
Asn-346 bears high-mannose type glycan structures.
- Subcellular localisation
- Nucleus, Nucleolus
Storage
- Shipped at conditions
- Dry Ice
- Appropriate short-term storage conditions
- -80°C
- Appropriate long-term storage conditions
- -80°C
This product is an active protein and may elicit a biological response in vivo, handle with caution.
Product promise
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4 product images
HPLC - Recombinant Human Carbonic Anhydrase 9 / CA9 Protein (Active) (ab290062)
HPLC analysis of ab290062
Biological Activity - Recombinant Human Carbonic Anhydrase 9 / CA9 Protein (Active) (ab290062)
Fully active measured by its esterase activity. The specific activity is ≥ 200 pmol/min/ug.
Mass Spectrometry - Recombinant Human Carbonic Anhydrase 9 / CA9 Protein (Active) (ab290062)
Mass determination by ESI-TOF. Predicted MW is 38810.99 Da (+/-10 Da by ESI-TOF). Observed MW is 38812.95 Da.
SDS-PAGE - Recombinant Human Carbonic Anhydrase 9 / CA9 Protein (Active) (ab290062)
SDS-PAGE analysis of ab290062
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