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AB204183

Recombinant Human CARD14 protein

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Recombinant Human CARD14 protein is a Human Full Length protein, in the 2 to 434 aa range, expressed in Escherichia coli, with >90%, suitable for SDS-PAGE.

View Alternative Names

CARMA2, CARD14, Caspase recruitment domain-containing protein 14, CARD-containing MAGUK protein 2, Carma 2

Key facts

Purity

>90% SDS-PAGE

Expression system

Escherichia coli

Tags

T7 tag N-Terminus

Applications

SDS-PAGE

applications

Biologically active

No

Accession

Q9BXL6

Animal free

No

Carrier free

No

Species

Human

Storage buffer

pH: 8 Constituents: 0.32% Tris HCl

storage-buffer

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Sequence info

[{"sequence":"MASMTGGQQMGRGEFVSSCELELQEQSLRTASDQESGDEELNRLKEENEKLRSLTFSLAEKDILEQSLDEARGSRQELVERIHSLRERAVAAERQREQYWEEKEQTLLQFQKSKMACQLYREKVNALQAQVCELQKERDQAYSARDSAQREISQSLVEKDSLRRQVFELTDQVCELRTQLRQLQAEPPGVLKQEARTREPCPREKQRLVRMHAICPRDDSDCSLVSSTESQLLSDLSATSSRELVDSFRSSSPAPPSQQSLYKRVAEDFGEEPWSFSSCLEIPEGDPGALPGAKAGDPHLDYELLDTADLPQLESSLQPVSPGRLDVSESGVLMRRRPARRILSQVTMLAFQGDALLEQISVIGGNLTGIFIHRVTPGSAADQMALRPGTQIVMVSRARPLLSPGLLMGTVAAGGVTQADFTSPRRCRSTLGWASALSWADVKRSAHL","proteinLength":"Full Length","predictedMolecularWeight":"50 kDa","actualMolecularWeight":null,"aminoAcidEnd":434,"aminoAcidStart":2,"nature":"Recombinant","expressionSystem":"Escherichia coli","accessionNumber":"Q9BXL6","tags":[{"tag":"T7","terminus":"N-Terminus"}]}]
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-80°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
False
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

CARD14 also known as CARMA2 is a protein involved in the regulation of immune responses. Its molecular weight is approximately 120 kDa. This protein is expressed mainly in keratinocytes which are cells found in the epidermis of the skin. Mechanically CARD14 functions as a scaffold protein that facilitates the assembly of signalosomes. These structures are essential for transmitting important signals within cells that activate various pathways linked to inflammation and immune responses.
Biological function summary

CARD14 plays a significant role in the NF-kB signaling pathway activating it to promote inflammation and immune responses. CARD14 forms a complex with other proteins to facilitate the activation of this pathway which leads to the expression of genes involved in inflammation and immune responses. Through this complex formation CARD14 helps modulate both the innate and adaptive immune systems by influencing the behavior of immune cells making it critical for proper skin and immune function.

Pathways

CARD14 acts as a mediator within the NF-kB and MAPK pathways both vital for cellular responses to stress cytokines and pathogens. CARD14 interacts with other proteins such as BCL10 and MALT1 which are part of the CARD-CC/BCL10/MALT1 signalosome. This interaction helps transduce signals that promote the expression of pro-inflammatory cytokines and chemokines which are critical for mounting effective immune reactions.

CARD14 has strong connections to psoriasis vulgaris and pityriasis rubra pilaris. Mutations in the CARD14 gene are associated with these inflammatory skin conditions which are marked by excessive immune activation and chronic inflammation. CARD14 influences these diseases by affecting the expression of cytokines mediated through the NF-kB pathway. Its interaction with BCL10 further emphasizes its role in promoting inflammation contributing to the pathogenesis of these disorders.
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Specifications

Form

Liquid

Additional notes

ab 204183 was expressed in E.coli as inclusion bodies, refolded using a unique temperature shift inclusion body refolding technology and chromatographically purified.

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General info

Function

Acts as a scaffolding protein that can activate the inflammatory transcription factor NF-kappa-B and p38/JNK MAP kinase signaling pathways. Forms a signaling complex with BCL10 and MALT1, and activates MALT1 proteolytic activity and inflammatory gene expression. MALT1 is indispensable for CARD14-induced activation of NF-kappa-B and p38/JNK MAP kinases (PubMed : 11278692, PubMed : 21302310, PubMed : 27071417, PubMed : 27113748). May play a role in signaling mediated by TRAF2, TRAF3 and TRAF6 and protects cells against apoptosis.. Isoform 3. Not able to activate the inflammatory transcription factor NF-kappa-B and may function as a dominant negative regulator (PubMed : 21302310, PubMed : 26358359).

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Product protocols

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Target data

Acts as a scaffolding protein that can activate the inflammatory transcription factor NF-kappa-B and p38/JNK MAP kinase signaling pathways. Forms a signaling complex with BCL10 and MALT1, and activates MALT1 proteolytic activity and inflammatory gene expression. MALT1 is indispensable for CARD14-induced activation of NF-kappa-B and p38/JNK MAP kinases (PubMed : 11278692, PubMed : 21302310, PubMed : 27071417, PubMed : 27113748). May play a role in signaling mediated by TRAF2, TRAF3 and TRAF6 and protects cells against apoptosis.. Isoform 3. Not able to activate the inflammatory transcription factor NF-kappa-B and may function as a dominant negative regulator (PubMed : 21302310, PubMed : 26358359).
See full target information CARD14
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Product promise

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