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AB283299

Recombinant Human Cardiac Troponin I protein

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Recombinant Human Cardiac Troponin I protein is a Human Full Length protein in the 1 to 210 aa range with >90% purity and suitable for SDS-PAGE. The predicted molecular weight of ab283299 protein is 26.4 kDa.

- Save time and ensure accurate results - use our Cardiac Troponin I (cTnI) protein as a control

View Alternative Names

TNNC1, TNNI3, Cardiac troponin I

1 Images
SDS-PAGE - Recombinant Human Cardiac Troponin I protein (AB283299)
  • SDS-PAGE

Supplier Data

SDS-PAGE - Recombinant Human Cardiac Troponin I protein (AB283299)

Analysis of ab283299 (3 μg) by SDS-PAGE (15%) under reducing condition and visualized by coomassie blue stain.

Key facts

Purity

>90% SDS-PAGE

Expression system

Escherichia coli

Tags

His tag N-Terminus

Applications

SDS-PAGE

applications

Biologically active

No

Accession

P19429

Animal free

No

Carrier free

No

Species

Human

Storage buffer

pH: 7.5 Constituents: 69.243% Water, 30% Glycerol (glycerin, glycerine), 0.5% Sodium chloride, 0.24% Tris HCl, 0.015% (R*,R*)-1,4-Dimercaptobutan-2,3-diol, 0.002% PMSF

storage-buffer

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }

Product details

Ensure the validity of your result using our recombinant human Cardiac Troponin I (cTnI)/TNNI3 protein ab283299 as a positive control in SDS-PAGE.


Check out our protein gel staining guide for SDS-PAGE here

Sequence info

[{"sequence":"MGSSHHHHHHSSGLVPRGSHMGSMADGSSDAAREPRPAPAPIRRRSSNYRAYATEPHAKKKSKISASRKLQLKTLLLQIAKQELEREAEERRGEKGRALSTRCQPLELAGLGFAELQDLCRQLHARVDKVDEERYDIEAKVTKNITEIADLTQKIFDLRGKFKRPTLRRVRISADAMMQALLGARAKESLDLRAHLKQVKKEDTEKENREVGDWRKNIDALSGMEGRKKKFES","proteinLength":"Full Length","predictedMolecularWeight":"26.4 kDa","actualMolecularWeight":null,"aminoAcidEnd":210,"aminoAcidStart":1,"nature":"Recombinant","expressionSystem":"Escherichia coli","accessionNumber":"P19429","tags":[{"tag":"His","terminus":"N-Terminus"}]}]

Properties and storage information

Shipped at conditions
Dry Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
False

Specifications

Form

Liquid

General info

Function

Troponin I is the inhibitory subunit of troponin, the thin filament regulatory complex which confers calcium-sensitivity to striated muscle actomyosin ATPase activity.

Sequence similarities

Belongs to the troponin I family.

Post-translational modifications

Phosphorylated at Ser-42 and Ser-44 by PRKCE; phosphorylation increases myocardium contractile dysfunction (By similarity). Phosphorylated at Ser-23 and Ser-24 by PRKD1; phosphorylation reduces myofilament calcium sensitivity. Phosphorylated preferentially at Thr-31. Phosphorylation by STK4/MST1 alters its binding affinity to TNNC1 (cardiac Tn-C) and TNNT2 (cardiac Tn-T).

Product protocols

Target data

Troponin I is the inhibitory subunit of troponin, the thin filament regulatory complex which confers calcium-sensitivity to striated muscle actomyosin ATPase activity.
See full target information TNNI3

Product promise

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