Recombinant Human Cardiac Troponin I protein is a Human Full Length protein, in the 1 to 210 aa range, expressed in Escherichia coli, with >90% purity and suitable for SDS-PAGE.
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Key facts
- Purity
- >90% SDS-PAGE
- Expression system
- Escherichia coli
- Tags
- His tag N-Terminus
- Applications
- SDS-PAGE
- Biologically active
- No
Amino acid sequence
M G S S H H H H H H S S G L V P R G S H M G S M A D G S S D A A R E P R P A P A P I R R R S S N Y R A Y A T E P H A K K K S K I S A S R K L Q L K T L L L Q I A K Q E L E R E A E E R R G E K G R A L S T R C Q P L E L A G L G F A E L Q D L C R Q L H A R V D K V D E E R Y D I E A K V T K N I T E I A D L T Q K I F D L R G K F K R P T L R R V R I S A D A M M Q A L L G A R A K E S L D L R A H L K Q V K K E D T E K E N R E V G D W R K N I D A L S G M E G R K K K F E S
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application SDS-PAGE | Reactivity Reacts | Dilution info - | Notes - |
Target data
Function
Troponin I is the inhibitory subunit of troponin, the thin filament regulatory complex which confers calcium-sensitivity to striated muscle actomyosin ATPase activity.
Alternative names
TNNC1, TNNI3, Cardiac troponin I
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Recombinant Human Cardiac Troponin I protein is a Human Full Length protein, in the 1 to 210 aa range, expressed in Escherichia coli, with >90% purity and suitable for SDS-PAGE.
Key facts
- Purity
- >90% SDS-PAGE
- Expression system
- Escherichia coli
- Tags
- His tag N-Terminus
- Applications
- SDS-PAGE
- Biologically active
- No
- Accession
- P19429-1
- Animal free
- No
- Species
- Human
- Concentration
- Storage buffer
pH: 7.5
Constituents: 69.243% Water, 30% Glycerol (glycerin, glycerine), 0.5% Sodium chloride, 0.24% Tris HCl, 0.015% (R*,R*)-1,4-Dimercaptobutan-2,3-diol, 0.002% PMSF
Sequence info
Amino acid sequence
- M G S S H H H H H H S S G L V P R G S H M G S M A D G S S D A A R E P R P A P A P I R R R S S N Y R A Y A T E P H A K K K S K I S A S R K L Q L K T L L L Q I A K Q E L E R E A E E R R G E K G R A L S T R C Q P L E L A G L G F A E L Q D L C R Q L H A R V D K V D E E R Y D I E A K V T K N I T E I A D L T Q K I F D L R G K F K R P T L R R V R I S A D A M M Q A L L G A R A K E S L D L R A H L K Q V K K E D T E K E N R E V G D W R K N I D A L S G M E G R K K K F E S
- Accession
- P19429
- Protein length
- Full Length
- Predicted molecular weight
- 26.4 kDa
- Amino acids
- 1 to 210
- Nature
- Recombinant
- Tags
- His tag N-Terminus
Specifications
- Form
- Liquid
General info
- Function
Troponin I is the inhibitory subunit of troponin, the thin filament regulatory complex which confers calcium-sensitivity to striated muscle actomyosin ATPase activity.
- Sequence similarities
Belongs to the troponin I family.
- Post-translational modifications
Phosphorylated at Ser-42 and Ser-44 by PRKCE; phosphorylation increases myocardium contractile dysfunction (By similarity). Phosphorylated at Ser-23 and Ser-24 by PRKD1; phosphorylation reduces myofilament calcium sensitivity. Phosphorylated preferentially at Thr-31. Phosphorylation by STK4/MST1 alters its binding affinity to TNNC1 (cardiac Tn-C) and TNNT2 (cardiac Tn-T).
Storage
- Shipped at conditions
- Dry Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
0.25mg/ml (determined by Bradford assay)
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1 product image
SDS-PAGE - Recombinant Human Cardiac Troponin I protein (ab283299)
Analysis of ab283299 (3 μg) by SDS-PAGE (15%) under reducing condition and visualized by coomassie blue stain.
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Product protocols
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