AB86685

Recombinant Human Cardiac Troponin T protein

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(2 Publications)

Recombinant Human Cardiac Troponin T protein is a Human Full Length protein, in the 1 to 285 aa range, expressed in Escherichia coli, with >90%, suitable for SDS-PAGE, sELISA.

View Alternative Names

TnTc, Cardiac muscle troponin T, cTnT, TNNT2

2 Images

Sandwich ELISA - Recombinant Human Cardiac Troponin T protein (AB86685)

sELISA

Unknown

Sandwich ELISA - Recombinant Human Cardiac Troponin T protein (AB86685)

Standard Curve for Cardiac Troponin T (Analyte : Cardiac Troponin T protein (His tag) (ab86685)); dilution range 1pg/ml to 1ug/ml using Capture Antibody Mouse monoclonal [1F11] to Cardiac Troponin T (ab10214) at 5ug/ml and Detector Antibody Rabbit polyclonal to cardiac Troponin T (ab45932) at 0.5ug/ml.

SDS-PAGE - Recombinant Human Cardiac Troponin T protein (AB86685)

SDS-PAGE

Unknown

SDS-PAGE - Recombinant Human Cardiac Troponin T protein (AB86685)

15% SDS-PAGE showing ab86685(3μg).

Key facts

Purity

>90% SDS-PAGE

Expression system

Escherichia coli

Applications

SDS-PAGE, sELISA

applications

Biologically active

Accession

P45379

Animal free

Carrier free

Species

Human

Storage buffer

pH: 8 Preservative: 0.0006% Imidazole Constituents: 50% Glycerol (glycerin, glycerine), 1.16% Sodium chloride, 0.24% Tris HCl, 0.02% (R*,R*)-1,4-Dimercaptobutan-2,3-diol, 0.0017% PMSF

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }

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Sequence info

[{"sequence":"MGSSHHHHHHSSGLVPRGSHMSDIEEVVEEYEEEEQEEAAVEEQEEAAEEDAEAEAETEETRAEEDEEEEEAKEAEDGPMEESKPKPRSFMPNLVPPKIPDGERVDFDDIHRKRMEKDLNELQALIEAHFENRKKEEEELVSLKDRIERRRAERAEQQRIRNEREKERQNRLAEERARREEEENRRKAEDEARKKKALSNMMHFGGYIQKTERKSGKRQTEREKKKKILAERRKVLAIDHLNEDQLREKAKELWQSIYNLEAEKFDLQEKFKQQKYEINVLRNRINDNQKVSKTRGKAKVTGRWK","proteinLength":"Full Length","predictedMolecularWeight":"36.4 kDa","actualMolecularWeight":null,"aminoAcidEnd":285,"aminoAcidStart":1,"nature":"Recombinant","expressionSystem":"Escherichia coli","accessionNumber":"P45379","tags":[{"tag":"His","terminus":"N-Terminus"}]}]

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Properties and storage information

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

-20°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

False

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Specifications

Form

Liquid

Additional notes

ab86685 is purified using conventional chromatography techniques.

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General info

Function

Troponin T is the tropomyosin-binding subunit of troponin, the thin filament regulatory complex which confers calcium-sensitivity to striated muscle actomyosin ATPase activity.

Sequence similarities

Belongs to the troponin T family.

Post-translational modifications

Phosphorylation at Thr-213 by PRKCA induces significant reduction in myofilament calcium sensitivity and actomyosin ATPase activity.

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Product protocols

Visit the General protocols
Visit the Troubleshooting

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Target data

Troponin T is the tropomyosin-binding subunit of troponin, the thin filament regulatory complex which confers calcium-sensitivity to striated muscle actomyosin ATPase activity.

See full target information TNNT2

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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Nano letters 24:6069-6077 PubMed38739779

2024

Lateral Flow Assay Biotesting by Utilizing Plasmonic Nanoparticles Made of Inexpensive Metals─Replacing Colloidal Gold.

Applications

Unspecified application

Species

Unspecified reactive species

Veronica A Bahamondes Lorca,Oscar Ávalos-Ovando,Christoph Sikeler,Heini Ijäs,Eva Yazmin Santiago,Eli Skelton,Yong Wang,Ruiqi Yang,Katherine Leslee Asetre Cimatu,Olga Baturina,Zhewei Wang,Jundong Liu,Joseph M Slocik,Shiyong Wu,Dongling Ma,Andrei Pastukhov,Andrei V Kabashin,Martin E Kordesch,Alexander O Govorov

GeroScience 44:2025-2045 PubMed35034279

2022

Cardiac troponin T and autoimmunity in skeletal muscle aging.

Applications

Unspecified application

Species

Unspecified reactive species

Tan Zhang,Xin Feng,Juan Dong,Zherong Xu,Bo Feng,Karen M Haas,Peggy M Cawthon,Kristen M Beavers,Barbara Nicklas,Stephen Kritchevsky

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Recombinant Human Cardiac Troponin T protein

Sandwich ELISA - Recombinant Human Cardiac Troponin T protein (AB86685)

SDS-PAGE - Recombinant Human Cardiac Troponin T protein (AB86685)

Key facts

Purity

Expression system

Tags

Applications

Biologically active

Accession

Animal free

Carrier free

Species

Storage buffer

Reactivity data

Sequence info

Properties and storage information

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Specifications

Form

Additional notes

General info

Function

Sequence similarities

Post-translational modifications

Product protocols

Target data

Publications (2)

Complementary Products

Primary Antibodies

Proteins & Peptides

Assay Kits

Proteins & Peptides

Product promise