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AB316587

Recombinant Human Cardiac Troponin T Protein Standard (His tag)

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Recombinant Human Cardiac Troponin T Protein Standard (His tag) is a Human Fragment protein, expressed in Escherichia coli, with >80%, suitable for SDS-PAGE, sELISA.

View Alternative Names

TnTc, Cardiac muscle troponin T, cTnT, TNNT2

2 Images
Sandwich ELISA - Recombinant Human Cardiac Troponin T Protein Standard (His tag) (AB316587)
  • sELISA

Supplier Data

Sandwich ELISA - Recombinant Human Cardiac Troponin T Protein Standard (His tag) (AB316587)

Sandwich ELISA with the capture antibody dilution at 2 μg/mL and detector antibody dilution at 0.5 μg/mL.

SDS-PAGE - Recombinant Human Cardiac Troponin T Protein Standard (His tag) (AB316587)
  • SDS-PAGE

Supplier Data

SDS-PAGE - Recombinant Human Cardiac Troponin T Protein Standard (His tag) (AB316587)

SDS-PAGE analysis of ab316587 under reducing conditions for 2ug protein

Key facts

Purity

>80% SDS-PAGE

Expression system

Escherichia coli

Tags

His tag C-Terminus

Applications

SDS-PAGE, sELISA

applications

Biologically active

No

Accession

P45379

Animal free

Yes

Carrier free

No

Species

Human

Storage buffer

pH: 7.3 - 7.5 Constituents: 2.922% Sodium chloride, 0.64107% disodium;hydrogen phosphate;dodecahydrate, 0.02858% Potassium phosphate monobasic

storage-buffer

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Complementary Products

Reagents, Controls & Accessories

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Prestained Protein Ladder - Mid-range molecular weight (10 - 180 kDa)

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Primary Antibodies

AB209813

Anti-Cardiac Troponin T antibody [EPR20266]

RabMAb|Recombinant

Immunocytochemistry/ Immunofluorescence - Anti-Cardiac Troponin T antibody [EPR20266] (AB209813)

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Proteins & Peptides

AB283299

Recombinant Human Cardiac Troponin I protein (His tag N-Terminus)

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Assay Kits

AB223860

Human Cardiac Troponin T ELISA Kit

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ELISA - Human Cardiac Troponin T ELISA Kit (AB223860)

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Proteins & Peptides

AB316391

Recombinant Human NT-proBNP Protein Standard (His tag)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

While the standard is the same as the one provided in the corresponding SimpleStep ELISA Kit, it cannot be treated as the consumable provided with our SimpleStep ELISA Kit due to differences in its concentration calibration.

Abcam guarantee that this protein standard is suitable for use in a sandwich ELISA. Individual results may vary due to differences in technique, laboratory equipment, buffers, and other experimental factors. The detection range provided for this protein standard is based on initial sandwich ELISA validation data.

The protein concentration is the concentration after validation on our sandwich ELISA platform. This Standard protein is guaranteed to work with our Capture and Detector antibodies in sELISA. Please contact our Scientific Support team to know which antibody pair is suitable for this protein.

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Sequence info

[{"sequence":null,"proteinLength":"Fragment","predictedMolecularWeight":"21.1 kDa","actualMolecularWeight":null,"aminoAcidEnd":0,"aminoAcidStart":0,"nature":"Recombinant","expressionSystem":"Escherichia coli","accessionNumber":"P45379","tags":[{"tag":"His","terminus":"C-Terminus"}]}]
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Properties and storage information

Shipped at conditions
Dry Ice
Appropriate short-term storage conditions
-80°C
Appropriate long-term storage conditions
-80°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
False
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Specifications

Form

Liquid

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General info

Function

Troponin T is the tropomyosin-binding subunit of troponin, the thin filament regulatory complex which confers calcium-sensitivity to striated muscle actomyosin ATPase activity.

Sequence similarities

Belongs to the troponin T family.

Post-translational modifications

Phosphorylation at Thr-213 by PRKCA induces significant reduction in myofilament calcium sensitivity and actomyosin ATPase activity.

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Product protocols

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Target data

Troponin T is the tropomyosin-binding subunit of troponin, the thin filament regulatory complex which confers calcium-sensitivity to striated muscle actomyosin ATPase activity.
See full target information TNNT2
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com