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AB102102

Recombinant human Casein Kinase 1 alpha protein

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(1 Publication)

Recombinant human Casein Kinase 1 alpha protein is a Human Full Length protein, in the 1 to 337 aa range, expressed in Baculovirus infected Sf9, with >80% purity and suitable for western blot and Functional studies. The predicted molecular weight of ab102102 protein is 62. kDa.

- Save time and ensure accurate results - use our human Casein Kinase 1 alpha (CK1 alpha 1) protein as a control
- Optimal protein bioactivity and stability
- Available in different sizes to fit your experimental needs

View Alternative Names

Casein kinase I isoform alpha, CKI-alpha, CK1, CSNK1A1

4 Images
Functional Studies - Recombinant human Casein Kinase 1 alpha protein (AB102102)
  • FuncS

Unknown

Functional Studies - Recombinant human Casein Kinase 1 alpha protein (AB102102)

The specific activity of Casein Kinase 1 alpha (ab102102) was determined to be 69 nmol/min/mg as per activity assay protocol and was equivalent to 120 nmol/min/mg as per radiometric assay

Functional Studies - Recombinant human Casein Kinase 1 alpha protein (AB102102)
  • FuncS

Unknown

Functional Studies - Recombinant human Casein Kinase 1 alpha protein (AB102102)

The specific activity of ab102102 was determined to be 120 nmol/min/mg.

SDS-PAGE - Recombinant human Casein Kinase 1 alpha protein (AB102102)
  • SDS-PAGE

Unknown

SDS-PAGE - Recombinant human Casein Kinase 1 alpha protein (AB102102)

ab102102 was determined to be >80% pure by densitometry. MWt 62 kDa.

SDS-PAGE - Recombinant human Casein Kinase 1 alpha protein (AB102102)
  • SDS-PAGE

Unknown

SDS-PAGE - Recombinant human Casein Kinase 1 alpha protein (AB102102)

SDS PAGE analysis of ab102102

Key facts

Purity

>80% Densitometry

Expression system

Baculovirus infected Sf9 cells

Tags

GST tag N-Terminus

Applications

FuncS, WB

applications

Biologically active

Yes

Biological activity

Specific activity is 120 nmol/min/mg

Accession

P48729

Animal free

No

Carrier free

No

Species

Human

Storage buffer

pH: 7.5 Constituents: 25% Glycerol (glycerin, glycerine), 0.87% Sodium chloride, 0.79% Tris HCl, 0.307% Glutathione, 0.00385% (R*,R*)-1,4-Dimercaptobutan-2,3-diol, 0.00292% EDTA, 0.00174% PMSF

storage-buffer

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "WB": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "FuncS": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Ensure the validity of your result using our bioactive recombinant human Casein Kinase 1 alpha (CK1 alpha 1) protein ab102102 as a positive control in western blot.


Check out of western blot protocol for more information here
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Sequence info

[{"linker":null,"sequence":"","proteinLength":"Full Length","predictedMolecularWeight":"62 kDa","actualMolecularWeight":null,"aminoAcidEnd":337,"aminoAcidStart":1,"nature":"Recombinant","expressionSystem":null,"accessionNumber":"P48729","tags":[{"tag":"GST","terminus":"N-Terminus"}]}]
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Properties and storage information

Shipped at conditions
Dry Ice
Appropriate short-term storage conditions
-80°C
Appropriate long-term storage conditions
-80°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
True
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Specifications

Form

Liquid

Additional notes

The purity of Casein Kinase 1 alpha protein (Active) was >80% as assessed by densitometry. Affinity purified.

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General info

Function

Casein kinases are operationally defined by their preferential utilization of acidic proteins such as caseins as substrates (PubMed : 11955436, PubMed : 1409656, PubMed : 18305108, PubMed : 23902688). It can phosphorylate a large number of proteins (PubMed : 11955436, PubMed : 1409656, PubMed : 18305108, PubMed : 23902688). Participates in Wnt signaling (PubMed : 11955436). Phosphorylates CTNNB1 at 'Ser-45' (PubMed : 11955436). May phosphorylate PER1 and PER2 (By similarity). May play a role in segregating chromosomes during mitosis (PubMed : 1409656). May play a role in keratin cytoskeleton disassembly and thereby, it may regulate epithelial cell migration (PubMed : 23902688). Acts as a positive regulator of mTORC1 and mTORC2 signaling in response to nutrients by mediating phosphorylation of DEPTOR inhibitor (PubMed : 22017875, PubMed : 22017877). Acts as an inhibitor of NLRP3 inflammasome assembly by mediating phosphorylation of NLRP3 (By similarity).

Sequence similarities

Belongs to the protein kinase superfamily. CK1 Ser/Thr protein kinase family. Casein kinase I subfamily.

Post-translational modifications

Phosphorylated by MTOR in response to mitogenic stimulation, leading to its activation.

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Product protocols

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Target data

Casein kinases are operationally defined by their preferential utilization of acidic proteins such as caseins as substrates (PubMed : 11955436, PubMed : 1409656, PubMed : 18305108, PubMed : 23902688). It can phosphorylate a large number of proteins (PubMed : 11955436, PubMed : 1409656, PubMed : 18305108, PubMed : 23902688). Participates in Wnt signaling (PubMed : 11955436). Phosphorylates CTNNB1 at 'Ser-45' (PubMed : 11955436). May phosphorylate PER1 and PER2 (By similarity). May play a role in segregating chromosomes during mitosis (PubMed : 1409656). May play a role in keratin cytoskeleton disassembly and thereby, it may regulate epithelial cell migration (PubMed : 23902688). Acts as a positive regulator of mTORC1 and mTORC2 signaling in response to nutrients by mediating phosphorylation of DEPTOR inhibitor (PubMed : 22017875, PubMed : 22017877). Acts as an inhibitor of NLRP3 inflammasome assembly by mediating phosphorylation of NLRP3 (By similarity).
See full target information CSNK1A1
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Life science alliance 5: PubMed36271509

2022

Dose-dependent phosphorylation and activation of Hh pathway transcription factors.

Applications

Unspecified application

Species

Unspecified reactive species

Mengmeng Zhou,Yuhong Han,Bing Wang,Yong Suk Cho,Jin Jiang
View all publications
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Product promise

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