JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB104025

Recombinant human Casein Kinase 1 gamma 2 protein (GST tag N-Terminus)

Be the first to review this product! Submit a review

|

(0 Publication)

Recombinant human Casein Kinase 1 gamma 2 protein (GST tag N-Terminus) is a Human Full Length protein, in the 1 to 415 aa range, expressed in Baculovirus infected Sf9 cells, with >80%, suitable for WB, FuncS.

View Alternative Names

CK1G2, CSNK1G2, Casein kinase I isoform gamma-2, CKI-gamma 2

2 Images
Functional Studies - Recombinant human Casein Kinase 1 gamma 2 protein (AB104025)
  • FuncS

Unknown

Functional Studies - Recombinant human Casein Kinase 1 gamma 2 protein (AB104025)

The Specific activity of ab104025 was determined to be 30 nmol/min/mg.

SDS-PAGE - Recombinant human Casein Kinase 1 gamma 2 protein (AB104025)
  • SDS-PAGE

Unknown

SDS-PAGE - Recombinant human Casein Kinase 1 gamma 2 protein (AB104025)

SDS-PAGE showing ab104025 at approximately 73kDa.

Key facts

Purity

>80% SDS-PAGE

Purity was determined to be >85% by densitometry. Affinity purified.

Expression system

Baculovirus infected Sf9 cells

Tags

GST tag N-Terminus

Applications

WB, FuncS

applications

Biologically active

Yes

Biological activity

The Specific activity of ab104025 was determined to be 30 nmol/min/mg.

Accession

Animal free

No

Carrier free

No

Species

Human

Storage buffer

pH: 7.5 Constituents: 25% Glycerol (glycerin, glycerine), 0.87% Sodium chloride, 0.79% Tris HCl, 0.307% Glutathione, 0.00385% (R*,R*)-1,4-Dimercaptobutan-2,3-diol, 0.00292% EDTA, 0.00174% PMSF

storage-buffer

style
left-column

Complementary Products

Proteins & Peptides

AB102102

Recombinant human Casein Kinase 1 alpha protein (GST tag N-Terminus)

Functional Studies - Recombinant human Casein Kinase 1 alpha protein (AB102102)

  • 0
  • 0
Proteins & Peptides

AB56268

Recombinant human cAMP Protein Kinase Catalytic subunit (GST tag N-Terminus)

Functional Studies - Recombinant human cAMP Protein Kinase Catalytic subunit (AB56268)

  • 0
  • 0
Proteins & Peptides

AB60837

Recombinant human PKA beta (catalytic subunit) protein (GST tag N-Terminus)

Functional Studies - Recombinant human PKA beta (catalytic subunit) protein (AB60837)

  • 0
  • 0
Proteins & Peptides

AB105125

Recombinant human CSNK2A2 protein (GST tag N-Terminus)

Functional Studies - Recombinant human CSNK2A2 protein (AB105125)

  • 0
  • 0
style
left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "WB": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "FuncS": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
style
left-column
style
left-column

Sequence info

[{"linker":null,"sequence":"","proteinLength":"Full Length","predictedMolecularWeight":"73 kDa","actualMolecularWeight":null,"aminoAcidEnd":415,"aminoAcidStart":1,"nature":"Recombinant","expressionSystem":null,"accessionNumber":"P78368","tags":[{"tag":"GST","terminus":"N-Terminus"}]}]
style
left-column

Properties and storage information

Form
Liquid
Purification technique
Affinity purification
Shipped at conditions
Dry Ice
Appropriate short-term storage conditions
-80°C
Appropriate long-term storage conditions
-80°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
True
style
left-column
style
left-column

General info

Function

Serine/threonine-protein kinase. Casein kinases are operationally defined by their preferential utilization of acidic proteins such as caseins as substrates. It can phosphorylate a large number of proteins. Participates in Wnt signaling (By similarity). Phosphorylates COL4A3BP/CERT, MTA1 and SMAD3. SMAD3 phosphorylation promotes its ligand-dependent ubiquitination and subsequent proteasome degradation, thus inhibiting SMAD3-mediated TGF-beta responses. Hyperphosphorylation of the serine-repeat motif of COL4A3BP/CERT leads to its inactivation by dissociation from the Golgi complex, thus down-regulating ER-to-Golgi transport of ceramide and sphingomyelin synthesis. Triggers PER1 proteasomal degradation probably through phosphorylation (PubMed : 15077195, PubMed : 15917222, PubMed : 18794808, PubMed : 19005213). Involved in brain development and vesicular trafficking and neurotransmitter releasing from small synaptic vesicles. Regulates fast synaptic transmission mediated by glutamate (By similarity). Involved in regulation of reactive oxygen species (ROS) levels (PubMed : 37099597).

Sequence similarities

Belongs to the protein kinase superfamily. CK1 Ser/Thr protein kinase family. Casein kinase I subfamily.

Post-translational modifications

Autophosphorylated (PubMed:18794808). Phosphorylated by aPKC which promotes dissociation from the cell cortex (PubMed:26481050).

style
left-column

Product protocols

style
left-column

Target data

Serine/threonine-protein kinase. Casein kinases are operationally defined by their preferential utilization of acidic proteins such as caseins as substrates. It can phosphorylate a large number of proteins. Participates in Wnt signaling (By similarity). Phosphorylates COL4A3BP/CERT, MTA1 and SMAD3. SMAD3 phosphorylation promotes its ligand-dependent ubiquitination and subsequent proteasome degradation, thus inhibiting SMAD3-mediated TGF-beta responses. Hyperphosphorylation of the serine-repeat motif of COL4A3BP/CERT leads to its inactivation by dissociation from the Golgi complex, thus down-regulating ER-to-Golgi transport of ceramide and sphingomyelin synthesis. Triggers PER1 proteasomal degradation probably through phosphorylation (PubMed : 15077195, PubMed : 15917222, PubMed : 18794808, PubMed : 19005213). Involved in brain development and vesicular trafficking and neurotransmitter releasing from small synaptic vesicles. Regulates fast synaptic transmission mediated by glutamate (By similarity). Involved in regulation of reactive oxygen species (ROS) levels (PubMed : 37099597).
See full target information CSNK1G2
style
left-column
style
left-column
style
right-column

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com