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AB131707

Recombinant human CASK protein

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Recombinant human CASK protein is a Human Fragment protein, in the 1 to 564 aa range, expressed in Baculovirus infected Sf9 cells, with >95%, suitable for SDS-PAGE, WB, FuncS.

View Alternative Names

LIN2, CASK, Peripheral plasma membrane protein CASK, hCASK, Calcium/calmodulin-dependent serine protein kinase, Protein lin-2 homolog

4 Images
Functional Studies - Recombinant human CASK protein (AB131707)
  • FuncS

Unknown

Functional Studies - Recombinant human CASK protein (AB131707)

The specific activity of CASK (ab131707) was determined to be 0.24 nmol/min/mg as per activity assay protocol and was equivalent to 3.5 nmol/min/mg as per radiometric assay

SDS-PAGE - Recombinant human CASK protein (AB131707)
  • SDS-PAGE

Supplier Data

SDS-PAGE - Recombinant human CASK protein (AB131707)

SDS PAGE analysis of ab131707

Functional Studies - Recombinant human CASK protein (AB131707)
  • FuncS

Unknown

Functional Studies - Recombinant human CASK protein (AB131707)

The specific activity of ab131707 was determined to be 3 nmol/min/mg.

SDS-PAGE - Recombinant human CASK protein (AB131707)
  • SDS-PAGE

Unknown

SDS-PAGE - Recombinant human CASK protein (AB131707)

The purity of ab131707 was determined to be >95% by densitometry.

Key facts

Purity

>95% SDS-PAGE

Expression system

Baculovirus infected Sf9 cells

Tags

GST tag N-Terminus

Applications

SDS-PAGE, WB, FuncS

applications

Biologically active

Yes

Biological activity

The specific activity of ab131707 was determined to be 3 nmol/min/mg.

Accession

O14936

Animal free

No

Carrier free

No

Species

Human

Storage buffer

pH: 7.5 Constituents: 25% Glycerol (glycerin, glycerine), 0.88% Sodium chloride, 0.79% Tris HCl, 0.31% Glutathione, 0.005% (R*,R*)-1,4-Dimercaptobutan-2,3-diol, 0.003% EDTA, 0.002% PMSF

storage-buffer

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "WB": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "FuncS": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

ab204874 (PKI-alpha peptide) can be utilized as a substrate for assessing kinase activity
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Sequence info

[{"sequence":"","proteinLength":"Fragment","predictedMolecularWeight":"95 kDa","actualMolecularWeight":null,"aminoAcidEnd":564,"aminoAcidStart":1,"nature":"Recombinant","expressionSystem":null,"accessionNumber":"O14936","tags":[{"tag":"GST","terminus":"N-Terminus"}]}]
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Properties and storage information

Shipped at conditions
Dry Ice
Appropriate short-term storage conditions
-80°C
Appropriate long-term storage conditions
-80°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
True
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

CASK also known as calcium/calmodulin-dependent serine protein kinase is a multifunctional protein with approximately 112 kDa mass. It is involved in intracellular signaling and synaptic function. CASK mainly localizes at the cell membrane but also exists in the cytoplasm and nucleus. This protein widely expresses in the brain and various tissues including the kidney and heart. Its alternative name includes LIN-2 which refers to its homolog in C. elegans.
Biological function summary

CASK acts as a scaffold protein facilitating protein interactions and signal transduction. It forms part of larger complexes such as the MAGUK family supporting the stability and function of cell adhesion molecules. CASK interacts with proteins like neurexins and leukocyte common antigen-related family proteins playing an important role in synaptic formation and maintenance. Its activity involves phosphorylation which impacts neural development mechanisms.

Pathways

One can find CASK within the neuronal signaling and cell adhesion pathways. It serves a role in neurotransmission processes and synaptic plasticity closely interacting with proteins like neurexin and Mint1. CASK participates in the arrangement of synaptic vesicle exocytosis and influences synaptic strength. As a scaffold it integrates signals from different pathways enabling a coordinated cellular response.

Abnormalities in CASK expression or function link to neurological disorders such as mental retardation and epilepsy. Mutations in the CASK gene may lead to a disorder known as mental retardation and microcephaly with pontine and cerebellar hypoplasia (MICPCH) characterized by developmental issues in brain structure. Moreover CASK dysfunction can relate to autism spectrum disorders where it interacts with proteins like neurexin affecting synaptic connectivity and transmission.
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Specifications

Form

Liquid

Additional notes

Assessed by densitometry. Affinity purified.

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General info

Function

Multidomain scaffolding Mg(2+)-independent protein kinase that catalyzes the phosphotransfer from ATP to proteins such as NRXN1, and plays a role in synaptic transmembrane protein anchoring and ion channel trafficking (PubMed : 18423203). Contributes to neural development and regulation of gene expression via interaction with the transcription factor TBR1. Binds to cell-surface proteins, including amyloid precursor protein, neurexins and syndecans. May mediate a link between the extracellular matrix and the actin cytoskeleton via its interaction with syndecan and with the actin/spectrin-binding protein 4.1. Component of the LIN-10-LIN-2-LIN-7 complex, which associates with the motor protein KIF17 to transport vesicles containing N-methyl-D-aspartate (NMDA) receptor subunit NR2B along microtubules (By similarity).

Sequence similarities

In the N-terminal section; belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. CaMK subfamily.. Belongs to the MAGUK family.

Subcellular localisation

Nucleus

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Multidomain scaffolding Mg(2+)-independent protein kinase that catalyzes the phosphotransfer from ATP to proteins such as NRXN1, and plays a role in synaptic transmembrane protein anchoring and ion channel trafficking (PubMed : 18423203). Contributes to neural development and regulation of gene expression via interaction with the transcription factor TBR1. Binds to cell-surface proteins, including amyloid precursor protein, neurexins and syndecans. May mediate a link between the extracellular matrix and the actin cytoskeleton via its interaction with syndecan and with the actin/spectrin-binding protein 4.1. Component of the LIN-10-LIN-2-LIN-7 complex, which associates with the motor protein KIF17 to transport vesicles containing N-methyl-D-aspartate (NMDA) receptor subunit NR2B along microtubules (By similarity).
See full target information CASK
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions
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Associated Products

Select an associated product type
Alternative Product
Proteins & Peptides

AB199574

Recombinant Human CASK protein (His tag)

proteins-peptides

recombinant-human-cask-protein-his-tag-ab199574

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