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AB52109

Recombinant human Caspase-5 protein

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Recombinant human Caspase-5 protein is a Human Full Length protein, expressed in Escherichia coli, with >95%, suitable for SDS-PAGE, FuncS.

View Alternative Names

ICH3, CASP5, Caspase-5, CASP-5, ICE(rel)-III, Protease ICH-3, Protease TY

Key facts

Purity

>95% SDS-PAGE

Expression system

Escherichia coli

Tags

Tag free

Applications

SDS-PAGE, FuncS

applications

Biologically active

Yes

Biological activity

SPECIFIC ACTIVITY: 5000 units/mg

Accession

P51878

Animal free

No

Carrier free

No

Species

Human

Reconstitution

Reconstitute to 1 unit per µl in ddH2O. Lyophilized caspase-5 is stable for 1 year at –70°C. Following reconstitution, the enzyme should be aliquoted and immediately stored at –70°C.

Storage buffer

Constituents: Water

storage-buffer

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "FuncS": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p>1 unit/assay for analyzing caspase activity is recommended. One unit of the recombinant caspase-5 is the enzyme activity that cleaves 1 nmol of the caspase substrate WEHD-pNA (pNA: pnitroanaline) per hour at 37°C, in a reaction solution containing 50 mM Hepes, pH 7.2, 50 mM NaCl, 0.1% Chaps, 10 mM EDTA, 5% Glycerol, and 10 mM DTT.</p>" } } }
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Product details

This product is manufactured by BioVision, an Abcam company and was previously called 1085 Caspase-5, human recombinant. 1085-100 is the same size as the 100 unit size of ab52109.

Active caspase-5 is useful in studying enzyme regulation, determining target substrates, screening caspase inhibitors, or as a positive control in caspase activity assays. Recommend using 1 unit/assay for analyzing caspase activity.

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Sequence info

[{"sequence":"","proteinLength":"Full Length","predictedMolecularWeight":null,"actualMolecularWeight":null,"aminoAcidEnd":0,"aminoAcidStart":0,"nature":"Recombinant","expressionSystem":null,"accessionNumber":"P51878","tags":[]}]
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate long-term storage conditions
-80°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
True
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Specifications

Form

Lyophilized

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General info

Function

Thiol protease that acts as a mediator of programmed cell death (PubMed : 28314590, PubMed : 29898893). Initiates pyroptosis, a programmed lytic cell death pathway through cleavage of Gasdermin-D (GSDMD) : cleavage releases the N-terminal gasdermin moiety (Gasdermin-D, N-terminal) that binds to membranes and forms pores, triggering pyroptosis (PubMed : 29898893). Also mediates cleavage and maturation of IL18 (PubMed : 37993714). Cleavage of GSDMD and IL18 is not strictly dependent on the consensus cleavage site but depends on an exosite interface on CASP4 (PubMed : 37993714). During non-canonical inflammasome activation, cuts CGAS and may play a role in the regulation of antiviral innate immune activation (PubMed : 28314590).

Sequence similarities

Belongs to the peptidase C14A family.

Post-translational modifications

The two subunits are derived from the precursor sequence by an autocatalytic mechanism.

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Product protocols

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Target data

Thiol protease that acts as a mediator of programmed cell death (PubMed : 28314590, PubMed : 29898893). Initiates pyroptosis, a programmed lytic cell death pathway through cleavage of Gasdermin-D (GSDMD) : cleavage releases the N-terminal gasdermin moiety (Gasdermin-D, N-terminal) that binds to membranes and forms pores, triggering pyroptosis (PubMed : 29898893). Also mediates cleavage and maturation of IL18 (PubMed : 37993714). Cleavage of GSDMD and IL18 is not strictly dependent on the consensus cleavage site but depends on an exosite interface on CASP4 (PubMed : 37993714). During non-canonical inflammasome activation, cuts CGAS and may play a role in the regulation of antiviral innate immune activation (PubMed : 28314590).
See full target information CASP5
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