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Recombinant human Caspase-8 protein is a Human Fragment protein, in the 200 to 496 aa range, expressed in Escherichia coli, with >=99% purity and suitable for SDS-PAGE, FuncS.

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Images

SDS-PAGE - Recombinant human Caspase-8 protein (AB198070), expandable thumbnail
  • Functional Studies - Recombinant human Caspase-8 protein (AB198070), expandable thumbnail

Publications

Key facts

Purity

>=99% SDS-PAGE

Expression system

Escherichia coli

Tags

His tag C-Terminus

Applications

SDS-PAGE, FuncS

Biologically active

Yes

Amino acid sequence

M N K S L L K I I N D Y E E F S K G E E L C G V M T I S D S P R E Q D S E S Q T L D K V Y Q M K S K P R G Y C L I I N N H N F A K A R E K V P K L H S I R D R N G T H L D A G A L T T T F E E L H F E I K P H D D C T V E Q I Y E I L K I Y Q L M D H S N M D C F I C C I L S H G D K G I I Y G T D G Q E A P I Y E L T S Q F T G L K C P S L A G K P K V F F I Q A C Q G D N Y Q K G I P V E T D S E E Q P Y L E M D L S S P Q T R Y I P D E A D F L L G M A T V N N C V S Y R N P A E G T W Y I Q S L C Q S L R E R C P R G D D I L T I L T E V N Y E V S N K D D K K N M G K Q M P Q P T F T L R K K L V F P S D H H H H H H

Reactivity data

Application

SDS-PAGE

Reactivity

Reacts

Dilution info

-

Notes

-

Application

FuncS

Reactivity

Reacts

Dilution info

-

Notes

-

Associated Products

Select an associated product type

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Target data

Function

Thiol protease that plays a key role in programmed cell death by acting as a molecular switch for apoptosis, necroptosis and pyroptosis, and is required to prevent tissue damage during embryonic development and adulthood (PubMed:23516580, PubMed:35338844, PubMed:35446120, PubMed:8681376, PubMed:8681377, PubMed:8962078, PubMed:9006941, PubMed:9184224). Initiator protease that induces extrinsic apoptosis by mediating cleavage and activation of effector caspases responsible for FAS/CD95-mediated and TNFRSF1A-induced cell death (PubMed:23516580, PubMed:35338844, PubMed:35446120, PubMed:8681376, PubMed:8681377, PubMed:8962078, PubMed:9006941, PubMed:9184224). Cleaves and activates effector caspases CASP3, CASP4, CASP6, CASP7, CASP9 and CASP10 (PubMed:16916640, PubMed:8962078, PubMed:9006941). Binding to the adapter molecule FADD recruits it to either receptor FAS/TNFRSF6 or TNFRSF1A (PubMed:8681376, PubMed:8681377). The resulting aggregate called the death-inducing signaling complex (DISC) performs CASP8 proteolytic activation (PubMed:9184224). The active dimeric enzyme is then liberated from the DISC and free to activate downstream apoptotic proteases (PubMed:9184224). Proteolytic fragments of the N-terminal propeptide (termed CAP3, CAP5 and CAP6) are likely retained in the DISC (PubMed:9184224). In addition to extrinsic apoptosis, also acts as a negative regulator of necroptosis: acts by cleaving RIPK1 at 'Asp-324', which is crucial to inhibit RIPK1 kinase activity, limiting TNF-induced apoptosis, necroptosis and inflammatory response (PubMed:31827280, PubMed:31827281). Also able to initiate pyroptosis by mediating cleavage and activation of gasdermin-C and -D (GSDMC and GSDMD, respectively): gasdermin cleavage promotes release of the N-terminal moiety that binds to membranes and forms pores, triggering pyroptosis (PubMed:32929201, PubMed:34012073). Initiates pyroptosis following inactivation of MAP3K7/TAK1 (By similarity). Also acts as a regulator of innate immunity by mediating cleavage and inactivation of N4BP1 downstream of TLR3 or TLR4, thereby promoting cytokine production (By similarity). May participate in the Granzyme B (GZMB) cell death pathways (PubMed:8755496). Cleaves PARP1 and PARP2 (PubMed:8681376). Independent of its protease activity, promotes cell migration following phosphorylation at Tyr-380 (PubMed:18216014, PubMed:27109099).Isoform 5Lacks the catalytic site and may interfere with the pro-apoptotic activity of the complex.Isoform 6Lacks the catalytic site and may interfere with the pro-apoptotic activity of the complex.Isoform 7Lacks the catalytic site and may interfere with the pro-apoptotic activity of the complex (Probable). Acts as an inhibitor of the caspase cascade (PubMed:12010809).Isoform 8Lacks the catalytic site and may interfere with the pro-apoptotic activity of the complex.

Alternative names

Recommended products

Recombinant human Caspase-8 protein is a Human Fragment protein, in the 200 to 496 aa range, expressed in Escherichia coli, with >=99% purity and suitable for SDS-PAGE, FuncS.

Key facts

Purity

>=99% SDS-PAGE

Expression system

Escherichia coli

Applications

SDS-PAGE, FuncS

Biological activity

Specific Activity: ≥ 1145 pmol/min/μg

Accession
Q14790-1
Animal free

No

Species

Human

Concentration
Loading...
Storage buffer

pH: 8
Preservative: 1.36% Imidazole
Constituents: 20% Glycerol (glycerin, glycerine), 0.64% Sodium chloride, 0.63% Tris HCl, 0.05% (R*,R*)-1,4-Dimercaptobutan-2,3-diol, 0.02% Potassium chloride

Sequence info

Amino acid sequence

M N K S L L K I I N D Y E E F S K G E E L C G V M T I S D S P R E Q D S E S Q T L D K V Y Q M K S K P R G Y C L I I N N H N F A K A R E K V P K L H S I R D R N G T H L D A G A L T T T F E E L H F E I K P H D D C T V E Q I Y E I L K I Y Q L M D H S N M D C F I C C I L S H G D K G I I Y G T D G Q E A P I Y E L T S Q F T G L K C P S L A G K P K V F F I Q A C Q G D N Y Q K G I P V E T D S E E Q P Y L E M D L S S P Q T R Y I P D E A D F L L G M A T V N N C V S Y R N P A E G T W Y I Q S L C Q S L R E R C P R G D D I L T I L T E V N Y E V S N K D D K K N M G K Q M P Q P T F T L R K K L V F P S D H H H H H H

Accession

Q14790

Protein length

Fragment

Predicted molecular weight

34.5 kDa

Amino acids

200 to 496

Nature

Recombinant

Tags

His tag C-Terminus

Specifications

Form

Liquid

General info

Function

Thiol protease that plays a key role in programmed cell death by acting as a molecular switch for apoptosis, necroptosis and pyroptosis, and is required to prevent tissue damage during embryonic development and adulthood (PubMed:23516580, PubMed:35338844, PubMed:35446120, PubMed:8681376, PubMed:8681377, PubMed:8962078, PubMed:9006941, PubMed:9184224). Initiator protease that induces extrinsic apoptosis by mediating cleavage and activation of effector caspases responsible for FAS/CD95-mediated and TNFRSF1A-induced cell death (PubMed:23516580, PubMed:35338844, PubMed:35446120, PubMed:8681376, PubMed:8681377, PubMed:8962078, PubMed:9006941, PubMed:9184224). Cleaves and activates effector caspases CASP3, CASP4, CASP6, CASP7, CASP9 and CASP10 (PubMed:16916640, PubMed:8962078, PubMed:9006941). Binding to the adapter molecule FADD recruits it to either receptor FAS/TNFRSF6 or TNFRSF1A (PubMed:8681376, PubMed:8681377). The resulting aggregate called the death-inducing signaling complex (DISC) performs CASP8 proteolytic activation (PubMed:9184224). The active dimeric enzyme is then liberated from the DISC and free to activate downstream apoptotic proteases (PubMed:9184224). Proteolytic fragments of the N-terminal propeptide (termed CAP3, CAP5 and CAP6) are likely retained in the DISC (PubMed:9184224). In addition to extrinsic apoptosis, also acts as a negative regulator of necroptosis: acts by cleaving RIPK1 at 'Asp-324', which is crucial to inhibit RIPK1 kinase activity, limiting TNF-induced apoptosis, necroptosis and inflammatory response (PubMed:31827280, PubMed:31827281). Also able to initiate pyroptosis by mediating cleavage and activation of gasdermin-C and -D (GSDMC and GSDMD, respectively): gasdermin cleavage promotes release of the N-terminal moiety that binds to membranes and forms pores, triggering pyroptosis (PubMed:32929201, PubMed:34012073). Initiates pyroptosis following inactivation of MAP3K7/TAK1 (By similarity). Also acts as a regulator of innate immunity by mediating cleavage and inactivation of N4BP1 downstream of TLR3 or TLR4, thereby promoting cytokine production (By similarity). May participate in the Granzyme B (GZMB) cell death pathways (PubMed:8755496). Cleaves PARP1 and PARP2 (PubMed:8681376). Independent of its protease activity, promotes cell migration following phosphorylation at Tyr-380 (PubMed:18216014, PubMed:27109099).

Sequence similarities

Belongs to the peptidase C14A family.

Post-translational modifications

Generation of the p10 and p18 subunits requires association with the death-inducing signaling complex (DISC), whereas additional processing is likely due to the autocatalytic activity of the activated protease. GZMB and CASP10 can be involved in these processing events.

Subcellular localisation

Nucleus

Storage

Shipped at conditions

Dry Ice

Appropriate short-term storage conditions

-80°C

Appropriate long-term storage conditions

-80°C

Storage information

Avoid freeze / thaw cycle

This product is an active protein and may elicit a biological response in vivo, handle with caution.

Notes

Peptide notes: Autocleaved between the prodomain and large protease subunit (p18). The cleavage product, p30, contains both the large (p18) and small (p10 [His-tag]) protease subunits. p30 is further processed to the p18 and p10 subunits, which dimerize to form active enzyme.

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2 product images

  • SDS-PAGE - Recombinant human Caspase-8 protein (ab198070), expandable thumbnail

    SDS-PAGE - Recombinant human Caspase-8 protein (ab198070)

    4-20% SDS-PAGE analysis of ab198070 (7 μg) with Coomassie staining.

  • Functional Studies - Recombinant human Caspase-8 protein (ab198070), expandable thumbnail

    Functional Studies - Recombinant human Caspase-8 protein (ab198070)

    Example of specific activity of ab198070.

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Product protocols

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