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AB114274

Recombinant Human CaSR protein

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Recombinant Human CaSR protein is a Human Fragment protein, in the 21 to 130 aa range, expressed in Wheat germ, suitable for SDS-PAGE, ELISA, WB.

View Alternative Names

GPRC2A, PCAR1, CASR, Extracellular calcium-sensing receptor, CaR, CaSR, hCasR, Parathyroid cell calcium-sensing receptor 1, PCaR1

1 Images
SDS-PAGE - Recombinant Human CaSR protein (AB114274)
  • SDS-PAGE

Unknown

SDS-PAGE - Recombinant Human CaSR protein (AB114274)

12.5% SDS-PAGE analysis of ab114274 stained with Coomassie Blue.

Key facts

Expression system

Wheat germ

Tags

Tag free

Applications

WB, ELISA, SDS-PAGE

applications

Biologically active

No

Accession

P41180

Animal free

No

Carrier free

No

Species

Human

Storage buffer

pH: 8 Constituents: 0.79% Tris HCl, 0.3% Glutathione

storage-buffer

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "ELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "WB": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p>(recombinant protein)</p>" } } }
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Product details

This product was previously labelled as Calcium Sensing Receptor.
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Sequence info

[{"sequence":"GPDQRAQKKGDIILGGLFPIHFGVAAKDQDLKSRPESVECIRYNFRGFRWLQAMIFAIEEINSSPALLPNLTLGYRIFDTCNTVSKALEATLSFVAQNKIDSLNLDEFCN","proteinLength":"Fragment","predictedMolecularWeight":"37.73 kDa","actualMolecularWeight":null,"aminoAcidEnd":130,"aminoAcidStart":21,"nature":"Recombinant","expressionSystem":"Wheat germ","accessionNumber":"P41180","tags":[]}]
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Properties and storage information

Shipped at conditions
Dry Ice
Appropriate short-term storage conditions
-80°C
Appropriate long-term storage conditions
-80°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
False
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The calcium-sensing receptor often referred to as CaSR is a critical G-protein coupled receptor involved in calcium ion homeostasis. Also known as CASR this receptor detects changes in extracellular calcium concentration and modulates signaling pathways accordingly. The CaSR protein has a molecular weight of approximately 120 kDa. It is mainly expressed in the parathyroid gland kidney and several other tissues playing a significant role in regulating serum calcium levels.
Biological function summary

The calcium-sensing receptor modulates parathyroid hormone secretion and renal calcium excretion in response to calcium fluctuations. Although not typically part of a larger protein complex it interacts with several other proteins to perform its functions efficiently. CaSR ensures the stability of calcium concentration which is essential for bone health muscle contraction and nerve function.

Pathways

Several other proteins work alongside the calcium-sensing receptor in the calcium signaling and parathyroid hormone secretion pathways. In particular CaSR influences the MAPK pathway which impacts cell growth and differentiation. Phosphate transporters and calbindins are related to CaSR within these pathways indicating its broad scope in calcium metabolism.

The calcium-sensing receptor is closely associated with conditions like hypercalcemia and autosomal dominant hypocalcemia. CaSR mutations can result in dysfunctional calcium regulation impacting parathyroid hormone levels. The relationship between CaSR and parathyroid hormone-related peptide highlights its significance in maintaining calcium balance and preventing skeletal and neurological complications.
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Specifications

Form

Liquid

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General info

Function

G-protein-coupled receptor that senses changes in the extracellular concentration of calcium ions and plays a key role in maintaining calcium homeostasis (PubMed : 17555508, PubMed : 19789209, PubMed : 21566075, PubMed : 22114145, PubMed : 22789683, PubMed : 23966241, PubMed : 25104082, PubMed : 25292184, PubMed : 25766501, PubMed : 26386835, PubMed : 7759551, PubMed : 8636323, PubMed : 8702647, PubMed : 8878438). Senses fluctuations in the circulating calcium concentration and modulates the production of parathyroid hormone (PTH) in parathyroid glands (By similarity). The activity of this receptor is mediated by a G-protein that activates a phosphatidylinositol-calcium second messenger system (PubMed : 7759551). The G-protein-coupled receptor activity is activated by a co-agonist mechanism : aromatic amino acids, such as Trp or Phe, act concertedly with divalent cations, such as calcium or magnesium, to achieve full receptor activation (PubMed : 27386547, PubMed : 27434672).

Sequence similarities

Belongs to the G-protein coupled receptor 3 family.

Post-translational modifications

N-glycosylated.. Ubiquitinated by RNF19A; which induces proteasomal degradation.

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Product protocols

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Target data

G-protein-coupled receptor that senses changes in the extracellular concentration of calcium ions and plays a key role in maintaining calcium homeostasis (PubMed : 17555508, PubMed : 19789209, PubMed : 21566075, PubMed : 22114145, PubMed : 22789683, PubMed : 23966241, PubMed : 25104082, PubMed : 25292184, PubMed : 25766501, PubMed : 26386835, PubMed : 7759551, PubMed : 8636323, PubMed : 8702647, PubMed : 8878438). Senses fluctuations in the circulating calcium concentration and modulates the production of parathyroid hormone (PTH) in parathyroid glands (By similarity). The activity of this receptor is mediated by a G-protein that activates a phosphatidylinositol-calcium second messenger system (PubMed : 7759551). The G-protein-coupled receptor activity is activated by a co-agonist mechanism : aromatic amino acids, such as Trp or Phe, act concertedly with divalent cations, such as calcium or magnesium, to achieve full receptor activation (PubMed : 27386547, PubMed : 27434672).
See full target information CASR
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