Recombinant Human CASS4 protein is a Human Full Length protein, in the 1 to 672 aa range, expressed in Wheat germ and suitable for ELISA, WB.
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Key facts
- Expression system
- Wheat germ
- Tags
- GST tag N-Terminus
- Applications
- ELISA, WB
- Biologically active
- No
Amino acid sequence
M K G T G I M D C A P K A L L A R A L Y D N C P D C S D E L A F S R G D I L T I L E Q H V P E S E G W W K C L L H G R Q G L A P A N R L Q I L T E V A A D R P C P P F L R G L E E A P A S S E E T Y Q V P T L P R P P T P G P V Y E Q M R S W A E G P Q P P T A Q V Y E F P D P P T S A R I I C E K T L S F P K Q A I L T L P R P V R A S L P T L P S Q V Y D V P T Q H R G P V V L K E P E K Q Q L Y D I P A S P K K A G L H P P D S Q A S G Q G V P L I S V T T L R R G G Y S T L P N P Q K S E W I Y D T P V S P G K A S V R N T P L T S F A E E S R P H A L P S S S S T F Y N P P S G R S R S L T P Q L N N N V P M Q K K L S L P E I P S Y G F L V P R G T F P L D E D V S Y K V P S S F L I P R V E Q Q N T K P N I Y D I P K A T S S V S Q A G K E L E K A K E V S E N S A G H N S S W F S R R T T S P S P E P D R L S G S S S D S R A S I V S S C S T T S T D D S S S S S S E E S A K E L S L D L D V A K E T V M A L Q H K V V S S V A G L M L F V S R K W R F R D Y L E A N I D A I H R S T D H I E E S V R E F L D F A R G V H G T A C N L T D S N L Q N R I R D Q M Q T I S N S Y R I L L E T K E S L D N R N W P L E V L V T D S V Q N S P D D L E R F V M V A R M L P E D I K R F A S I V I A N G R L L F K R N C E K E E T V Q L T P N A E F K C E K Y I Q P P Q R E T E S H Q K S T P S T K Q R E D E H S S E L L K K N R A N I C G Q V S S E F Q V I E K G A S I V T W S S G Y
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application ELISA | Reactivity Reacts | Dilution info - | Notes - |
Application WB | Reactivity Reacts | Dilution info - | Notes - |
Target data
Function
Docking protein that plays a role in tyrosine kinase-based signaling related to cell adhesion and cell spreading. Regulates PTK2/FAK1 activity, focal adhesion integrity, and cell spreading.
Alternative names
C20orf32, HEFL, CASS4, Cas scaffolding protein family member 4, HEF-like protein, HEF1-EFS-p130Cas-like protein, HEPL
Recommended products
Recombinant Human CASS4 protein is a Human Full Length protein, in the 1 to 672 aa range, expressed in Wheat germ and suitable for ELISA, WB.
Key facts
- Expression system
- Wheat germ
- Tags
- GST tag N-Terminus
- Applications
- ELISA, WB
- Biologically active
- No
- Accession
- Q9NQ75-1
- Animal free
- No
- Species
- Human
- Concentration
- Storage buffer
pH: 8
Constituents: 0.79% Tris HCl, 0.31% Glutathione
Sequence info
Amino acid sequence
- M K G T G I M D C A P K A L L A R A L Y D N C P D C S D E L A F S R G D I L T I L E Q H V P E S E G W W K C L L H G R Q G L A P A N R L Q I L T E V A A D R P C P P F L R G L E E A P A S S E E T Y Q V P T L P R P P T P G P V Y E Q M R S W A E G P Q P P T A Q V Y E F P D P P T S A R I I C E K T L S F P K Q A I L T L P R P V R A S L P T L P S Q V Y D V P T Q H R G P V V L K E P E K Q Q L Y D I P A S P K K A G L H P P D S Q A S G Q G V P L I S V T T L R R G G Y S T L P N P Q K S E W I Y D T P V S P G K A S V R N T P L T S F A E E S R P H A L P S S S S T F Y N P P S G R S R S L T P Q L N N N V P M Q K K L S L P E I P S Y G F L V P R G T F P L D E D V S Y K V P S S F L I P R V E Q Q N T K P N I Y D I P K A T S S V S Q A G K E L E K A K E V S E N S A G H N S S W F S R R T T S P S P E P D R L S G S S S D S R A S I V S S C S T T S T D D S S S S S S E E S A K E L S L D L D V A K E T V M A L Q H K V V S S V A G L M L F V S R K W R F R D Y L E A N I D A I H R S T D H I E E S V R E F L D F A R G V H G T A C N L T D S N L Q N R I R D Q M Q T I S N S Y R I L L E T K E S L D N R N W P L E V L V T D S V Q N S P D D L E R F V M V A R M L P E D I K R F A S I V I A N G R L L F K R N C E K E E T V Q L T P N A E F K C E K Y I Q P P Q R E T E S H Q K S T P S T K Q R E D E H S S E L L K K N R A N I C G Q V S S E F Q V I E K G A S I V T W S S G Y
- Accession
- Q9NQ75
- Protein length
- Full Length
- Amino acids
- 1 to 672
- Nature
- Recombinant
- Tags
- GST tag N-Terminus
Specifications
- Form
- Liquid
General info
- Function
Docking protein that plays a role in tyrosine kinase-based signaling related to cell adhesion and cell spreading. Regulates PTK2/FAK1 activity, focal adhesion integrity, and cell spreading.
- Sequence similarities
Belongs to the CAS family.
- Post-translational modifications
Phosphorylated on tyrosines by SRC.
- Subcellular localisation
- Cytoskeleton
Storage
- Shipped at conditions
- Dry Ice
- Appropriate short-term storage conditions
- -80°C
- Appropriate long-term storage conditions
- -80°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
CASS4 also known as Cas scaffolding protein family member 4 or HEF1-L is involved in cellular signaling processes. It weighs approximately 78 kDa and is expressed in a variety of tissues including the brain lung and placenta. CASS4 participates in focal adhesion and acts as an adapter protein which interacts with other proteins to signal during changes in cell shape and mobility.
- Biological function summary
CASS4 acts in cell signaling by connecting integrins to the actin cytoskeleton. It is a part of the CAS protein family and functions within this complex to relay signals from the cell membrane to the interior. It influences cellular functions like adhesion migration and proliferation which are important for maintaining cellular architecture and responding to extracellular stimuli.
- Pathways
CASS4 engages in the focal adhesion and integrin signaling pathways. It associates with proteins like E-cadherin and FAK facilitating their functioning in these pathways. CASS4's interaction helps control signals that regulate actin cytoskeleton reorganization and cellular responses to the extracellular environment.
- Associated diseases and disorders
CASS4 has associations with cancer and Alzheimer’s disease. Alterations in CASS4 expression or function may contribute to tumor progression and metastasis potentially interacting with proteins such as p130Cas. In Alzheimer's disease changes in CASS4 could influence signaling pathways and affect neural cell functions sometimes relating to proteins like tau which are linked to neurodegenerative markers.
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1 product image
SDS-PAGE - Recombinant Human CASS4 protein (ab163474)
ab163474 on a 12.5% SDS-PAGE stained with Coomassie Blue.
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