Recombinant human Catalase protein (Active) is a Human Full Length protein, in the 1 to 552 aa range, expressed in Escherichia coli, with >=90% purity and suitable for SDS-PAGE, FuncS.
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Key facts
- Purity
- >=90% SDS-PAGE
- Expression system
- Escherichia coli
- Tags
- His tag N-Terminus
- Applications
- SDS-PAGE, FuncS
- Biologically active
- Yes
Amino acid sequence
M G S S H H H H H H S S G L V P R G S H M D D D D K A D S R D P A S D Q M Q H W K E Q R A A Q K A D V L T T G A G N P V G D K L N V I T V G P R G P L L V Q D V V F T D E M A H F D R E R I P E R V V H A K G A G A F G Y F E V T H D T K Y S K A K V F E H I G K K T P I A V R F S T V A G E S G S A D T V R D P R G F A V K F Y T E D G N W D L V G N N T P I F F I R D P I L F P S F I H S Q K R N P Q T H L K D P D M V W D F W S L R P E S L H Q V S F L F S D R G I P D G H R H M N G Y G S H T F L V N A N G E A V Y C K F H Y K T D Q G I K N L S V E D A A R L S Q E D P D Y G I R D L F N A I A T G K Y P S W T F Y I Q V M T F N Q A E T F P F N P F D L T K V W P H K D Y P L I P V G K L V L N R N P V N Y F A E V E Q I A F D P S N M P P G I E A S P D K M L G R L F A Y P D T H R H R L G P N Y L H I P V N C P Y R A R V A N Y Q R D G P M C M Q D N Q G G A P N Y Y P N S F G A P E Q Q P S A L E H S I Q Y S G E V R R F N T A N D D N V T Q V R A F Y V N V L N E E
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Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application SDS-PAGE | Reactivity Reacts | Dilution info - | Notes - |
Application FuncS | Reactivity Reacts | Dilution info - | Notes - |
Target data
Function
Catalyzes the degradation of hydrogen peroxide (H(2)O(2)) generated by peroxisomal oxidases to water and oxygen, thereby protecting cells from the toxic effects of hydrogen peroxide (PubMed:7882369). Promotes growth of cells including T-cells, B-cells, myeloid leukemia cells, melanoma cells, mastocytoma cells and normal and transformed fibroblast cells (PubMed:7882369).
Alternative names
Catalase, CAT
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Recombinant human Catalase protein (Active) is a Human Full Length protein, in the 1 to 552 aa range, expressed in Escherichia coli, with >=90% purity and suitable for SDS-PAGE, FuncS.
Key facts
- Purity
- >=90% SDS-PAGE
- Expression system
- Escherichia coli
- Tags
- His tag N-Terminus
- Applications
- SDS-PAGE, FuncS
- Biologically active
- Yes
- Biological activity
- Specific activity is >20,000 unit/mg
- Accession
- P04040-1
- Animal free
- No
- Carrier free
- Yes
- Species
- Human
- Reconstitution
- Reconstitute in water
- Concentration
- Storage buffer
pH: 8
Constituents: 10% Glycerol (glycerin, glycerine), 0.877% Sodium chloride, 0.606% Tris
Sequence info
Amino acid sequence
- M G S S H H H H H H S S G L V P R G S H M D D D D K A D S R D P A S D Q M Q H W K E Q R A A Q K A D V L T T G A G N P V G D K L N V I T V G P R G P L L V Q D V V F T D E M A H F D R E R I P E R V V H A K G A G A F G Y F E V T H D T K Y S K A K V F E H I G K K T P I A V R F S T V A G E S G S A D T V R D P R G F A V K F Y T E D G N W D L V G N N T P I F F I R D P I L F P S F I H S Q K R N P Q T H L K D P D M V W D F W S L R P E S L H Q V S F L F S D R G I P D G H R H M N G Y G S H T F L V N A N G E A V Y C K F H Y K T D Q G I K N L S V E D A A R L S Q E D P D Y G I R D L F N A I A T G K Y P S W T F Y I Q V M T F N Q A E T F P F N P F D L T K V W P H K D Y P L I P V G K L V L N R N P V N Y F A E V E Q I A F D P S N M P P G I E A S P D K M L G R L F A Y P D T H R H R L G P N Y L H I P V N C P Y R A R V A N Y Q R D G P M C M Q D N Q G G A P N Y Y P N S F G A P E Q Q P S A L E H S I Q Y S G E V R R F N T A N D D N V T Q V R A F Y V N V L N E E
- Accession
- P04040
- Protein length
- Full Length
- Predicted molecular weight
- 60 kDa
- Actual molecular weight
- 62.5 kDa
- Amino acids
- 1 to 552
- Nature
- Recombinant
- Tags
- His tag N-Terminus
Specifications
- Form
- Lyophilized
General info
- Function
Catalyzes the degradation of hydrogen peroxide (H(2)O(2)) generated by peroxisomal oxidases to water and oxygen, thereby protecting cells from the toxic effects of hydrogen peroxide (PubMed:7882369). Promotes growth of cells including T-cells, B-cells, myeloid leukemia cells, melanoma cells, mastocytoma cells and normal and transformed fibroblast cells (PubMed:7882369).
- Sequence similarities
Belongs to the catalase family.
- Subcellular localisation
- Peroxisome
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Storage information
- Avoid freeze / thaw cycle, Reconstitute for long term storage
This product is an active protein and may elicit a biological response in vivo, handle with caution.
Notes
This product is manufactured by BioVision, an Abcam company and was previously called P1159 Catalase, Human Recombinant. P1159-100 is the same size as the 100 µg size of ab286037.
CAT is a key antioxidant enzyme in the bodies’ defense against oxidative stress. Also, it is a heme enzyme that is present in the peroxisome of nearly all aerobic cells. CAT converts the reactive oxygen species hydrogen peroxide to water and oxygen and thereby mitigates the toxic effects of hydrogen peroxide.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Catalase also known as CAT is an enzyme that catalyzes the decomposition of hydrogen peroxide into water and oxygen. This enzyme has a molecular weight of approximately 240 kDa and typically forms a tetramer. Catalase mainly resides in peroxisomes functioning as a peroxisome marker. It expresses abundantly in the liver kidneys and erythrocytes where it plays significant roles in cellular protection against oxidative damage. The presence of Catalase makes it an ideal candidate for use in peroxisome staining and peroxisome image analysis in research.
- Biological function summary
Catalase contributes to antioxidant defense by breaking down hydrogen peroxide preventing cellular damage. In peroxisomes it works alongside other peroxisomal enzymes to maintain cell health and metabolic regulation. Catalase does not form a complex but interacts closely with other enzymes like superoxide dismutase which dismutates superoxide radicals into less harmful substances. Increased Catalase activity levels can be measured using Catalase activity kits and activity assays allowing us to learn about peroxisome function.
- Pathways
Hydrogen peroxide removal by Catalase is vital in the reactive oxygen species (ROS) metabolic process and plays a part in the cellular response to oxidative stress. Catalase interacts with the glutathione peroxidase pathway safeguarding cells from oxidative stress-related damage. Superoxide dismutase works synergistically with Catalase transforming superoxide anions into hydrogen peroxide before its decomposition by Catalase. These activities highlight the essential role Catalase plays in protecting cells from oxidative stress damage.
- Associated diseases and disorders
Catalase relates to conditions like acatalasemia and diabetes. Acatalasemia a condition caused by a deficiency of Catalase increases the risk of developing diabetes and other oxidative stress-related diseases. Mutations in the CAT gene can lead to decreased Catalase activity contributing to the onset of these conditions. Additionally Catalase works with other proteins like glutathione peroxidase in mitigating the effects of oxidative stress with deficiencies potentially exacerbating complications in diabetes management.
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1 product image
SDS-PAGE - Recombinant human Catalase protein (Active) (ab286037)
SDS-PAGE (4-20%) of ab286037:
Loaded under reducing conditions and stained with Coomassie Blue. Lanes are as follows: MMW markers, 1 and 2 μg – purified Catalase. The protein band shows a predicted MW of ~ 62.5 kDa.
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