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AB323128

Recombinant Human Cathepsin C Protein Standard (His tag)

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Recombinant Human Cathepsin C Protein Standard (His tag) is a Human Full Length protein, expressed in HEK 293 cells, with >80%, suitable for sELISA, SDS-PAGE.

View Alternative Names

CPPI, Dipeptidyl peptidase 1, Cathepsin C, Cathepsin J, Dipeptidyl peptidase I, Dipeptidyl transferase, DPP-I, DPPI

2 Images
Sandwich ELISA - Recombinant Human Cathepsin C Protein Standard (His tag) (AB323128)
  • sELISA

Supplier Data

Sandwich ELISA - Recombinant Human Cathepsin C Protein Standard (His tag) (AB323128)

Sandwich ELISA with the capture antibody dilution at 2 µg/mL and detector antibody dilution at 0.5 µg/mL.

SDS-PAGE - Recombinant Human Cathepsin C Protein Standard (His tag) (AB323128)
  • SDS-PAGE

Supplier Data

SDS-PAGE - Recombinant Human Cathepsin C Protein Standard (His tag) (AB323128)

SDS-PAGE analysis of ab323128 under reducing conditions for 2ug protein.

Key facts

Purity

>80% SDS-PAGE

Expression system

HEK 293 cells

Tags

His tag C-Terminus

Applications

sELISA, SDS-PAGE

applications

Biologically active

No

Accession

P53634

Animal free

Yes

Carrier free

No

Species

Human

Storage buffer

pH: 7.3 - 7.5 Constituents: 2.922% Sodium chloride, 0.64107% disodium;hydrogen phosphate;dodecahydrate, 0.02858% Potassium phosphate monobasic

storage-buffer

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

While the standard is the same as the one provided in the corresponding SimpleStep ELISA Kit, it cannot be treated as the consumable provided with our SimpleStep ELISA Kit due to differences in its concentration calibration.Abcam guarantee that this protein standard is suitable for use in a sandwich ELISA. Individual results may vary due to differences in technique, laboratory equipment, buffers, and other experimental factors. The detection range provided for this protein standard is based on initial sandwich ELISA validation data.The protein concentration is the concentration after validation on our sandwich ELISA platform. This Standard protein is guaranteed to work with our Capture and Detector antibodies in sELISA. Please contact our Scientific Support team to know which antibody pair is suitable for this protein.
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Sequence info

[{"sequence":null,"proteinLength":"Full Length","predictedMolecularWeight":"51.1 kDa","actualMolecularWeight":null,"aminoAcidEnd":0,"aminoAcidStart":0,"nature":"Recombinant","expressionSystem":"HEK 293 cells","accessionNumber":"P53634","tags":[{"tag":"His","terminus":"C-Terminus"}]}]
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Properties and storage information

Shipped at conditions
Dry Ice
Appropriate short-term storage conditions
-80°C
Appropriate long-term storage conditions
-80°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
False
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Specifications

Form

Liquid

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General info

Function

Thiol protease (PubMed : 1586157). Has dipeptidylpeptidase activity (PubMed : 1586157). Active against a broad range of dipeptide substrates composed of both polar and hydrophobic amino acids (PubMed : 1586157). Proline cannot occupy the P1 position and arginine cannot occupy the P2 position of the substrate (PubMed : 1586157). Can act as both an exopeptidase and endopeptidase (PubMed : 1586157). Activates serine proteases such as elastase, cathepsin G and granzymes A and B (PubMed : 8428921).

Sequence similarities

Belongs to the peptidase C1 family.

Post-translational modifications

N-glycosylated. While glycosylation at Asn-53, Asn-119 and Asn-276 is mediated by STT3A-containing complexes, glycosylation at Asn-29 is mediated STT3B-containing complexes.. In approximately 50% of the complexes the exclusion domain is cleaved at position 58 or 61. The two parts of the exclusion domain are held together by a disulfide bond.

Subcellular localisation

Lysosome

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Product protocols

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Target data

Thiol protease (PubMed : 1586157). Has dipeptidylpeptidase activity (PubMed : 1586157). Active against a broad range of dipeptide substrates composed of both polar and hydrophobic amino acids (PubMed : 1586157). Proline cannot occupy the P1 position and arginine cannot occupy the P2 position of the substrate (PubMed : 1586157). Can act as both an exopeptidase and endopeptidase (PubMed : 1586157). Activates serine proteases such as elastase, cathepsin G and granzymes A and B (PubMed : 8428921).
See full target information CTSC
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Product promise

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