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AB160540

Recombinant Human CBF1 interacting corepressor/CIR1 protein (GST tag N-Terminus)

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Recombinant Human CBF1 interacting corepressor/CIR1 protein (GST tag N-Terminus) is a Human Full Length protein, in the 1 to 450 aa range, expressed in Wheat germ, suitable for ELISA, WB.

View Alternative Names

CIR, CIR1, Corepressor of RBPJ and splicing regulator, CBF1-interacting corepressor, Corepressor interacting with RBPJ 1, Recepin

1 Images
SDS-PAGE - Recombinant Human CBF1 interacting corepressor/CIR1 protein (GST tag N-Terminus) (AB160540)
  • SDS-PAGE

Unknown

SDS-PAGE - Recombinant Human CBF1 interacting corepressor/CIR1 protein (GST tag N-Terminus) (AB160540)

ab160540 on a 12.5% SDS-PAGE stained with Coomassie Blue.

Key facts

Expression system

Wheat germ

Tags

GST tag N-Terminus

Applications

ELISA, WB

applications

Biologically active

No

Accession

Q86X95

Animal free

No

Carrier free

No

Species

Human

Storage buffer

pH: 8 Constituents: 0.79% Tris HCl, 0.31% Glutathione

storage-buffer

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Complementary Products

Primary Antibodies

AB229866

Anti-CBF1 interacting corepressor/CIR1 antibody - N-terminal

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CBF1 interacting corepressor/CIR1 antibody - N-terminal (AB229866)

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  • 0
Secondary Antibodies

AB150155

Donkey Anti-Rat IgG H&L (Alexa Fluor® 647) preadsorbed

Immunocytochemistry/ Immunofluorescence - Donkey Anti-Rat IgG H&L (Alexa Fluor® 647) preadsorbed (AB150155)

  • 5
  • 2
Proteins & Peptides

AB159886

Recombinant Human ZP2 protein (GST tag N-Terminus)

SDS-PAGE - Recombinant Human ZP2 protein (GST tag N-Terminus) (AB159886)

  • 0
  • 0
Proteins & Peptides

AB160078

Recombinant Human DBPA protein (GST tag N-Terminus)

SDS-PAGE - Recombinant Human DBPA protein (GST tag N-Terminus) (AB160078)

  • 0
  • 0
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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "ELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "WB": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

This product was previously labelled as CBF1 interacting corepressor.
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Sequence info

[{"sequence":"MGKSFANFMCKKDFHPASKSNIKKVWMAEQKISYDKKKQEELMQQYLKEQESYDNRLLMGDERVKNGLNFMYEAPPGAKKENKEKEETEGETEYKFEWQKGAPREKYAKDDMNIRDQPFGIQVRNVRCIKCHKWGHVNTDRECPLFGLSGINASSVPTDGSGPSMHPSELIAEMRNSGFALKRNVLGRNLTANDPSQEYVASEGEEDPEVEFLKSLTTKQKQKLLRKLDRLEKKKKKKDRKKKKFQKSRSKHKKHKSSSSSSSSSSSSSSTETSESSSESESNNKEKKIQRKKRKKNKCSGHNNSDSEEKDKSKKRKLHEELSSSHHNREKAKEKPRFLKHESSREDSKWSHSDSDKKSRTHKHSPEKRGSERKEGSSRSHGREERSRRSRSRSPGSYKQRETRKRAQRNPGEEQSRRNDSRSHGTDLYRGEKMYREHPGGTHTKVTQRE","proteinLength":"Full Length","predictedMolecularWeight":null,"actualMolecularWeight":null,"aminoAcidEnd":450,"aminoAcidStart":1,"nature":"Recombinant","expressionSystem":"Wheat germ","accessionNumber":"Q86X95","tags":[{"tag":"GST","terminus":"N-Terminus"}]}]
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Properties and storage information

Shipped at conditions
Dry Ice
Appropriate short-term storage conditions
-80°C
Appropriate long-term storage conditions
-80°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
False
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

CBF1 interacting corepressor also known as CIR1 is a coregulator that interacts with CBF1 an important transcriptional repressor. CIR1 forms a complex with CBF1 and other components to modulate transcriptional repression processes. The molecular mass of CIR1 is approximately 47 kDa. It expresses in various tissues throughout the body including the brain and lymphoid tissues. This broad expression pattern suggests a role in multiple cellular processes. CIR1 plays a central part in controlling gene expression by interacting with CBF1 and other transcriptional modulators.
Biological function summary

CIR1 serves as a mediator in transcriptional repression and is a part of the transducin-like enhancer of split (TLE) corepressor complex. This complex influences the activity of CBF1 by promoting chromatin modifications that lead to transcriptional repression. CIR1 contributes to finely regulating gene expression essential for maintaining cellular homeostasis. The TLE complex generally recruits CIR1 to target genes modulating the expression of genes involved in cell growth and differentiation.

Pathways

CIR1 modulates Notch signaling and Hedgehog pathways two critical signal transduction systems for cell fate determination and development. In the Notch signaling pathway CIR1 collaborates with CBF1 (also known as RBPJ or CSL) to repress target gene transcription until the pathway is activated. Interaction with Notch proteins results in pathway activation which is important for cell communication and differentiation. CIR1 indirectly affects the Hedgehog signaling pathway by influencing transcription factors that respond to this pathway highlighting its role in developmental processes and tumorigenesis.

CIR1 is associated with oncogenesis and neurological disorders. Dysregulation of Notch signaling involving CIR1 can contribute to various cancers by altering cell proliferation and apoptosis. In neurological conditions such as Alzheimer's disease CIR1's involvement in transcriptional repression may affect neuronal function by disrupting gene regulation. CIR1 also interacts with Presenilin proteins which are linked to Alzheimer's pathology indicating potential roles in disease progression. Understanding CIR1's function in these pathways can provide insights into therapeutic targets for related disorders.
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Specifications

Form

Liquid

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General info

Function

May modulate splice site selection during alternative splicing of pre-mRNAs (By similarity). Regulates transcription and acts as corepressor for RBPJ. Recruits RBPJ to the Sin3-histone deacetylase complex (HDAC). Required for RBPJ-mediated repression of transcription.

Post-translational modifications

Phosphorylated by NEK6.

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Product protocols

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Target data

May modulate splice site selection during alternative splicing of pre-mRNAs (By similarity). Regulates transcription and acts as corepressor for RBPJ. Recruits RBPJ to the Sin3-histone deacetylase complex (HDAC). Required for RBPJ-mediated repression of transcription.
See full target information CIR1
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Product promise

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