Recombinant Human CBFb protein is a Human Full Length protein, in the 1 to 182 aa range, expressed in Escherichia coli, with >=85% purity and suitable for SDS-PAGE, MS.
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- Nature communications 10:20712019The transcription factor CBFB suppresses breast cancer through orchestrating translation and transcription.Applications:Unspecified applicationReactive species:Unspecified reactive speciesNavdeep Malik et. al.PubMed 31061501
Key facts
- Purity
- >=85% SDS-PAGE
- Expression system
- Escherichia coli
- Tags
- His tag N-Terminus
- Applications
- SDS-PAGE, MS
- Biologically active
- No
Amino acid sequence
M G S S H H H H H H S S G L V P R G S H M P R V V P D Q R S K F E N E E F F R K L S R E C E I K Y T G F R D R P H E E R Q A R F Q N A C R D G R S E I A F V A T G T N L S L Q F F P A S W Q G E Q R Q T P S R E Y V D L E R E A G K V Y L K A P M I L N G V C V I W K G W I D L Q R L D G M G C L E F D E E R A Q Q E D A L A Q Q A F E E A R R R T R E F E D R D R S H R E E M E V R V S Q L L A V T G K K T T R P
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application SDS-PAGE | Reactivity Reacts | Dilution info - | Notes - |
Application MS | Reactivity Reacts | Dilution info - | Notes - |
Target data
Function
Forms the heterodimeric complex core-binding factor (CBF) with RUNX family proteins (RUNX1, RUNX2, and RUNX3). RUNX members modulate the transcription of their target genes through recognizing the core consensus binding sequence 5'-TGTGGT-3', or very rarely, 5'-TGCGGT-3', within their regulatory regions via their runt domain, while CBFB is a non-DNA-binding regulatory subunit that allosterically enhances the sequence-specific DNA-binding capacity of RUNX. The heterodimers bind to the core site of a number of enhancers and promoters, including murine leukemia virus, polyomavirus enhancer, T-cell receptor enhancers, LCK, IL3 and GM-CSF promoters. CBF complexes repress ZBTB7B transcription factor during cytotoxic (CD8+) T cell development. They bind to RUNX-binding sequence within the ZBTB7B locus acting as transcriptional silencer and allowing for cytotoxic T cell differentiation. (Microbial infection) Following infection, hijacked by the HIV-1 Vif protein, leading to the formation a cullin-5-RING E3 ubiquitin-protein ligase complex (ECS complex) that catalyzes ubiquitination and degradation of APOBEC3F and APOBEC3G (PubMed:22190037, PubMed:31792451, PubMed:36598981, PubMed:36754086, PubMed:37419875). The complex can also ubiquitinate APOBEC3H to some extent (PubMed:37640699). Association with HIV-1 Vif protein also inhibits the transcription coactivator activity of CBFB/CBF-beta (PubMed:22190037).
Alternative names
Core-binding factor subunit beta, CBF-beta, Polyomavirus enhancer-binding protein 2 beta subunit, SL3-3 enhancer factor 1 subunit beta, SL3/AKV core-binding factor beta subunit, PEA2-beta, PEBP2-beta, CBFB
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Recombinant Human CBFb protein is a Human Full Length protein, in the 1 to 182 aa range, expressed in Escherichia coli, with >=85% purity and suitable for SDS-PAGE, MS.
Key facts
- Purity
- >=85% SDS-PAGE
- Expression system
- Escherichia coli
- Tags
- His tag N-Terminus
- Applications
- SDS-PAGE, MS
- Biologically active
- No
- Accession
- Q13951-1
- Animal free
- No
- Species
- Human
- Concentration
- Storage buffer
pH: 6
Constituents: 10% Glycerol (glycerin, glycerine), 0.39% MES, 0.00174% PMSF
Sequence info
Amino acid sequence
- M G S S H H H H H H S S G L V P R G S H M P R V V P D Q R S K F E N E E F F R K L S R E C E I K Y T G F R D R P H E E R Q A R F Q N A C R D G R S E I A F V A T G T N L S L Q F F P A S W Q G E Q R Q T P S R E Y V D L E R E A G K V Y L K A P M I L N G V C V I W K G W I D L Q R L D G M G C L E F D E E R A Q Q E D A L A Q Q A F E E A R R R T R E F E D R D R S H R E E M E V R V S Q L L A V T G K K T T R P
- Accession
- Q13951
- Protein length
- Full Length
- Predicted molecular weight
- 23.6 kDa
- Amino acids
- 1 to 182
- Nature
- Recombinant
- Tags
- His tag N-Terminus
Specifications
- Form
- Liquid
- Additional notes
ab98252 was purified using conventional chromatography.
General info
- Function
Forms the heterodimeric complex core-binding factor (CBF) with RUNX family proteins (RUNX1, RUNX2, and RUNX3). RUNX members modulate the transcription of their target genes through recognizing the core consensus binding sequence 5'-TGTGGT-3', or very rarely, 5'-TGCGGT-3', within their regulatory regions via their runt domain, while CBFB is a non-DNA-binding regulatory subunit that allosterically enhances the sequence-specific DNA-binding capacity of RUNX. The heterodimers bind to the core site of a number of enhancers and promoters, including murine leukemia virus, polyomavirus enhancer, T-cell receptor enhancers, LCK, IL3 and GM-CSF promoters. CBF complexes repress ZBTB7B transcription factor during cytotoxic (CD8+) T cell development. They bind to RUNX-binding sequence within the ZBTB7B locus acting as transcriptional silencer and allowing for cytotoxic T cell differentiation.
- Sequence similarities
Belongs to the CBF-beta family.
- Subcellular localisation
- Nucleus
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- -20°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
CBFb also known as Core-Binding Factor Subunit Beta weighs approximately 22 kDa. It plays an important role in gene regulation by stabilizing the DNA binding of core-binding factor alpha (CBFA) subunits such as RUNX proteins. CBFb does not bind DNA directly. Instead it enhances the affinity of the RUNX family for their DNA targets. CBFb is broadly expressed in various tissues with high levels observed in hematopoietic cells.
- Biological function summary
CBFb forms a complex with RUNX transcription factors facilitating their function. The complex is essential for hematopoiesis as it regulates the expression of genes critical for blood cell differentiation and proliferation. CBFb is involved in bone development influencing the formation of bone-related genes. This CBFb/RUNX complex plays a role in regulating the cell cycle and apoptosis fundamental processes for maintaining cellular integrity.
- Pathways
More than one signaling pathway includes CBFb's involvement. Particularly it participates in the Wnt signaling pathway an important player in cell differentiation. Additionally CBFb/RUNX complex regulates genes downstream in the TGF-beta signaling pathway impacting cellular proliferation and differentiation. Through these pathways CBFb is linked to proteins like SMAD3 further emphasizing its role in mediating various biological processes.
- Associated diseases and disorders
Abnormalities in CBFb function connect to acute myeloid leukemia (AML). CBFb's interaction with RUNX1 can result in a chromosomal translocation that generates the CBFb-MYH11 fusion protein which disrupts normal hematopoiesis and contributes to leukemogenesis. Moreover CBFb variations associate with cleidocranial dysplasia a skeletal disorder through its interaction with the RUNX2 protein highlighting its significance in bone development.
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SDS-PAGE - Recombinant Human CBFb protein (ab98252)
15% SDS-PAGE analysis of 3μg ab98252
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