Recombinant Human CBL protein is a Human Full Length protein, in the 1 to 906 aa range, expressed in Wheat germ and suitable for SDS-PAGE, ELISA, WB.
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Key facts
- Expression system
Wheat germ
- Tags
Tag free
- Applications
SDS-PAGE, ELISA, WB
- Biologically active
No
Amino acid sequence
M A G N V K K S S G A G G G S G S G G S G S G G L I G L M K D A F Q P H H H H H H H L S P H P P G T V D K K M V E K C W K L M D K V V R L C Q N P K L A L K N S P P Y I L D L L P D T Y Q H L R T I L S R Y E G K M E T L G E N E Y F R V F M E N L M K K T K Q T I S L F K E G K E R M Y E E N S Q P R R N L T K L S L I F S H M L A E L K G I F P S G L F Q G D T F R I T K A D A A E F W R K A F G E K T I V P W K S F R Q A L H E V H P I S S G L E A M A L K S T I D L T C N D Y I S V F E F D I F T R L F Q P W S S L L R N W N S L A V T H P G Y M A F L T Y D E V K A R L Q K F I H K P G S Y I F R L S C T R L G Q W A I G Y V T A D G N I L Q T I P H N K P L F Q A L I D G F R E G F Y L F P D G R N Q N P D L T G L C E P T P Q D H I K V T Q E Q Y E L Y C E M G S T F Q L C K I C A E N D K D V K I E P C G H L M C T S C L T S W Q E S E G Q G C P F C R C E I K G T E P I V V D P F D P R G S G S L L R Q G A E G A P S P N Y D D D D D E R A D D T L F M M K E L A G A K V E R P P S P F S M A P Q A S L P P V P P R L D L L P Q R V C V P S S A S A L G T A S K A A S G S L H K D K P L P V P P T L R D L P P P P P P D R P Y S V G A E S R P Q R R P L P C T P G D C P S R D K L P P V P S S R L G D S W L P R P I P K V P V S A P S S S D P W T G R E L T N R H S L P F S L P S Q M E P R P D V P R L G S T F S L D T S M S M N S S P L V G P E C D H P K I K P S S S A N A I Y S L A A R P L P V P K L P P G E Q C E G E E D T E Y M T P S S R P L R P L D T S Q S S R A C D C D Q Q I D S C T Y E A M Y N I Q S Q A P S I T E S S T F G E G N L A A A H A N T G P E E S E N E D D G Y D V P K P P V P A V L A R R T L S D I S N A S S S F G W L S L D G D P T T N V T E G S Q V P E R P P K P F P R R I N S E R K A G S C Q Q G S G P A A S A A T A S P Q L S S E I E N L M S Q G Y S Y Q D I Q K A L V I A Q N N I E M A K N I L R E F V S I S S P A H V A T
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application SDS-PAGE | Reactivity Reacts | Dilution info - | Notes - |
Application ELISA | Reactivity Reacts | Dilution info - | Notes - |
Application WB | Reactivity Reacts | Dilution info - | Notes - |
Associated Products
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Target data
Function
Adapter protein that functions as a negative regulator of many signaling pathways that are triggered by activation of cell surface receptors. Acts as an E3 ubiquitin-protein ligase, which accepts ubiquitin from specific E2 ubiquitin-conjugating enzymes, and then transfers it to substrates promoting their degradation by the proteasome (PubMed:17094949). Ubiquitinates SPRY2 (PubMed:17094949, PubMed:17974561). Ubiquitinates EGFR (PubMed:17974561). Recognizes activated receptor tyrosine kinases, including KIT, FLT1, FGFR1, FGFR2, PDGFRA, PDGFRB, CSF1R, EPHA8 and KDR and terminates signaling. Recognizes membrane-bound HCK, SRC and other kinases of the SRC family and mediates their ubiquitination and degradation. Participates in signal transduction in hematopoietic cells. Plays an important role in the regulation of osteoblast differentiation and apoptosis. Essential for osteoclastic bone resorption. The 'Tyr-731' phosphorylated form induces the activation and recruitment of phosphatidylinositol 3-kinase to the cell membrane in a signaling pathway that is critical for osteoclast function. May be functionally coupled with the E2 ubiquitin-protein ligase UB2D3. In association with CBLB, required for proper feedback inhibition of ciliary platelet-derived growth factor receptor-alpha (PDGFRA) signaling pathway via ubiquitination and internalization of PDGFRA (By similarity).
Alternative names
E3 ubiquitin-protein ligase CBL, Casitas B-lineage lymphoma proto-oncogene, Proto-oncogene c-Cbl, RING finger protein 55, RING-type E3 ubiquitin transferase CBL, Signal transduction protein CBL, RNF55, CBL2, CBL
Recommended products
Recombinant Human CBL protein is a Human Full Length protein, in the 1 to 906 aa range, expressed in Wheat germ and suitable for SDS-PAGE, ELISA, WB.
Alternative names
E3 ubiquitin-protein ligase CBL, Casitas B-lineage lymphoma proto-oncogene, Proto-oncogene c-Cbl, RING finger protein 55, RING-type E3 ubiquitin transferase CBL, Signal transduction protein CBL, RNF55, CBL2, CBL
Key facts
- Expression system
Wheat germ
- Tags
Tag free
- Applications
SDS-PAGE, ELISA, WB
- Biologically active
No
- Accession
- P22681-1
- Animal free
No
- Species
Human
- Concentration
- Storage buffer
pH: 8
Constituents: 0.79% Tris HCl, 0.3% Glutathione
Sequence info
Amino acid sequence
M A G N V K K S S G A G G G S G S G G S G S G G L I G L M K D A F Q P H H H H H H H L S P H P P G T V D K K M V E K C W K L M D K V V R L C Q N P K L A L K N S P P Y I L D L L P D T Y Q H L R T I L S R Y E G K M E T L G E N E Y F R V F M E N L M K K T K Q T I S L F K E G K E R M Y E E N S Q P R R N L T K L S L I F S H M L A E L K G I F P S G L F Q G D T F R I T K A D A A E F W R K A F G E K T I V P W K S F R Q A L H E V H P I S S G L E A M A L K S T I D L T C N D Y I S V F E F D I F T R L F Q P W S S L L R N W N S L A V T H P G Y M A F L T Y D E V K A R L Q K F I H K P G S Y I F R L S C T R L G Q W A I G Y V T A D G N I L Q T I P H N K P L F Q A L I D G F R E G F Y L F P D G R N Q N P D L T G L C E P T P Q D H I K V T Q E Q Y E L Y C E M G S T F Q L C K I C A E N D K D V K I E P C G H L M C T S C L T S W Q E S E G Q G C P F C R C E I K G T E P I V V D P F D P R G S G S L L R Q G A E G A P S P N Y D D D D D E R A D D T L F M M K E L A G A K V E R P P S P F S M A P Q A S L P P V P P R L D L L P Q R V C V P S S A S A L G T A S K A A S G S L H K D K P L P V P P T L R D L P P P P P P D R P Y S V G A E S R P Q R R P L P C T P G D C P S R D K L P P V P S S R L G D S W L P R P I P K V P V S A P S S S D P W T G R E L T N R H S L P F S L P S Q M E P R P D V P R L G S T F S L D T S M S M N S S P L V G P E C D H P K I K P S S S A N A I Y S L A A R P L P V P K L P P G E Q C E G E E D T E Y M T P S S R P L R P L D T S Q S S R A C D C D Q Q I D S C T Y E A M Y N I Q S Q A P S I T E S S T F G E G N L A A A H A N T G P E E S E N E D D G Y D V P K P P V P A V L A R R T L S D I S N A S S S F G W L S L D G D P T T N V T E G S Q V P E R P P K P F P R R I N S E R K A G S C Q Q G S G P A A S A A T A S P Q L S S E I E N L M S Q G Y S Y Q D I Q K A L V I A Q N N I E M A K N I L R E F V S I S S P A H V A T
- Accession
- Protein length
Full Length
- Predicted molecular weight
125.73 kDa
- Amino acids
1 to 906
- Nature
Recombinant
Specifications
- Form
Liquid
General info
- Function
Adapter protein that functions as a negative regulator of many signaling pathways that are triggered by activation of cell surface receptors. Acts as an E3 ubiquitin-protein ligase, which accepts ubiquitin from specific E2 ubiquitin-conjugating enzymes, and then transfers it to substrates promoting their degradation by the proteasome (PubMed:17094949). Ubiquitinates SPRY2 (PubMed:17094949, PubMed:17974561). Ubiquitinates EGFR (PubMed:17974561). Recognizes activated receptor tyrosine kinases, including KIT, FLT1, FGFR1, FGFR2, PDGFRA, PDGFRB, CSF1R, EPHA8 and KDR and terminates signaling. Recognizes membrane-bound HCK, SRC and other kinases of the SRC family and mediates their ubiquitination and degradation. Participates in signal transduction in hematopoietic cells. Plays an important role in the regulation of osteoblast differentiation and apoptosis. Essential for osteoclastic bone resorption. The 'Tyr-731' phosphorylated form induces the activation and recruitment of phosphatidylinositol 3-kinase to the cell membrane in a signaling pathway that is critical for osteoclast function. May be functionally coupled with the E2 ubiquitin-protein ligase UB2D3. In association with CBLB, required for proper feedback inhibition of ciliary platelet-derived growth factor receptor-alpha (PDGFRA) signaling pathway via ubiquitination and internalization of PDGFRA (By similarity).
- Post-translational modifications
Phosphorylated on tyrosine residues by ALK, EGFR, SYK, FYN and ZAP70 (By similarity). Phosphorylated on tyrosine residues in response to FLT1 and KIT signaling. Phosphorylated on tyrosine residues by INSR and FGR. Phosphorylated on several tyrosine residues by constitutively activated FGFR3. Not phosphorylated at Tyr-731 by FGFR3. Phosphorylated on tyrosine residues by activated CSF1R, PDGFRA and PDGFRB. Phosphorylated on tyrosine residues by HCK.
Storage
- Shipped at conditions
Dry Ice
- Appropriate long-term storage conditions
-80°C
- Aliquoting information
Upon delivery aliquot
- Storage information
Avoid freeze / thaw cycle
Supplementary info
This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
The CBL protein also known as C-cbl or Casitas B-lineage lymphoma proto-oncogene serves as an E3 ubiquitin-protein ligase. This protein with a molecular mass of approximately 120-130 kDa plays a critical role in the regulation of receptor tyrosine kinases. It facilitates the polyubiquitination of activated receptor proteins targeting them for degradation in the proteasome. CBL is predominantly expressed in various tissues including the hematopoietic system. It is a significant component in the control of cell signaling and helps maintain cellular homeostasis.
- Biological function summary
The CBL protein acts as a negative regulator for several signaling pathways. It integrates into signaling complexes and modulates the activation of pathways by interacting with other proteins. CBL does not act alone; it commonly forms complexes with proteins such as Grb2 and p85 influencing downstream signaling influence in pathways. This regulation prevents excessive activation of signals that can lead to cellular proliferation or differentiation abnormally.
- Pathways
CBL integrates into the ubiquitin-proteasome system and the Ras signaling pathway. Within these pathways CBL works to modulate cell surface receptors ensuring that signaling threshold is not surpassed. The importance of CBL in the Ras signaling pathway is highlighted by its interactions with proteins like Ras-GAP and SHP2. Through these interactions CBL controls the rate of signal transduction affecting cellular responses such as growth survival and motility.
- Associated diseases and disorders
CBL involvement has been linked to several forms of cancer and immune deficiencies. Mutations or dysregulation of the CBL gene can lead to myelodysplastic syndromes and acute myeloid leukemia. In these conditions the interaction between CBL and other proteins such as JAK2 becomes dysregulated driving abnormal cell growth and survival. Furthermore CBL’s failure to properly degrade tyrosine kinase receptors can contribute to enhanced signaling fostering oncogenic processes.
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1 product image
SDS-PAGE - Recombinant Human CBL protein (ab114527)
12.5% SDS-PAGE analysis of Human CBL protein (ab114527). Stained with Coomassie Blue.
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