Recombinant Human CBP80 protein is a Human Full Length protein, in the 1 to 790 aa range, expressed in Wheat germ and suitable for SDS-PAGE, ELISA, WB.
Images
Key facts
- Expression system
- Wheat germ
- Tags
- Tag free
- Applications
- SDS-PAGE, ELISA, WB
- Biologically active
- No
Amino acid sequence
M S R R R H S D E N D G G Q P H K R R K T S D A N E T E D H L E S L I C K V G E K S A C S L E S N L E G L A G V L E A D L P N Y K S K I L R L L C T V A R L L P E K L T I Y T T L V G L L N A R N Y N F G G E F V E A M I R Q L K E S L K A N N Y N E A V Y L V R F L S D L V N C H V I A A P S M V A M F E N F V S V T Q E E D V P Q V R R D W Y V Y A F L S S L P W V G K E L Y E K K D A E M D R I F A N T E S Y L K R R Q K T H V P M L Q V W T A D K P H P Q E E Y L D C L W A Q I Q K L K K D R W Q E R H I L R P Y L A F D S I L C E A L Q H N L P P F T P P P H T E D S V Y P M P R V I F R M F D Y T D D P E G P V M P G S H S V E R F V I E E N L H C I I K S H W K E R K T C A A Q L V S Y P G K N K I P L N Y H I V E V I F A E L F Q L P A P P H I D V M Y T T L L I E L C K L Q P G S L P Q V L A Q A T E M L Y M R L D T M N T T C V D R F I N W F S H H L S N F Q F R W S W E D W S D C L S Q D P E S P K P K F V R E V L E K C M R L S Y H Q R I L D I V P P T F S A L C P A N P T C I Y K Y G D E S S N S L P G H S V A L C L A V A F K S K A T N D E I F S I L K D V P N P N Q D D D D D E G F S F N P L K I E V F V Q T L L H L A A K S F S H S F S A L A K F H E V F K T L A E S D E G K L H V L R V M F E V W R N H P Q M I A V L V D K M I R T Q I V D C A A V A N W I F S S E L S R D F T R L F V W E I L H S T I R K M N K H V L K I Q K E L E E A K E K L A R Q H K R R S D D D D R S S D R K D G V L E E Q I E R L Q E K V E S A Q S E Q K N L F L V I F Q R F I M I L T E H L V R C E T D G T S V L T P W Y K N C I E R L Q Q I F L Q H H Q I I Q Q Y M V T L E N L L F T A E L D P H I L A V F Q Q F C A L Q A
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application SDS-PAGE | Reactivity Reacts | Dilution info - | Notes - |
Application ELISA | Reactivity Reacts | Dilution info - | Notes - |
Application WB | Reactivity Reacts | Dilution info - | Notes (Recombinant protein) |
Target data
Function
Component of the cap-binding complex (CBC), which binds cotranscriptionally to the 5'-cap of pre-mRNAs and is involved in various processes such as pre-mRNA splicing, translation regulation, nonsense-mediated mRNA decay, RNA-mediated gene silencing (RNAi) by microRNAs (miRNAs) and mRNA export. The CBC complex is involved in mRNA export from the nucleus via its interaction with ALYREF/THOC4/ALY, leading to the recruitment of the mRNA export machinery to the 5'-end of mRNA and to mRNA export in a 5' to 3' direction through the nuclear pore. The CBC complex is also involved in mediating U snRNA and intronless mRNAs export from the nucleus. The CBC complex is essential for a pioneer round of mRNA translation, before steady state translation when the CBC complex is replaced by cytoplasmic cap-binding protein eIF4E. The pioneer round of mRNA translation mediated by the CBC complex plays a central role in nonsense-mediated mRNA decay (NMD), NMD only taking place in mRNAs bound to the CBC complex, but not on eIF4E-bound mRNAs. The CBC complex enhances NMD in mRNAs containing at least one exon-junction complex (EJC) via its interaction with UPF1, promoting the interaction between UPF1 and UPF2. The CBC complex is also involved in 'failsafe' NMD, which is independent of the EJC complex, while it does not participate in Staufen-mediated mRNA decay (SMD). During cell proliferation, the CBC complex is also involved in microRNAs (miRNAs) biogenesis via its interaction with SRRT/ARS2 and is required for miRNA-mediated RNA interference. The CBC complex also acts as a negative regulator of PARN, thereby acting as an inhibitor of mRNA deadenylation. In the CBC complex, NCBP1/CBP80 does not bind directly capped RNAs (m7GpppG-capped RNA) but is required to stabilize the movement of the N-terminal loop of NCBP2/CBP20 and lock the CBC into a high affinity cap-binding state with the cap structure. Associates with NCBP3 to form an alternative cap-binding complex (CBC) which plays a key role in mRNA export and is particularly important in cellular stress situations such as virus infections. The conventional CBC with NCBP2 binds both small nuclear RNA (snRNA) and messenger (mRNA) and is involved in their export from the nucleus whereas the alternative CBC with NCBP3 does not bind snRNA and associates only with mRNA thereby playing a role only in mRNA export. NCBP1/CBP80 is required for cell growth and viability (PubMed:26382858).
Alternative names
CBP80, NCBP, NCBP1, Nuclear cap-binding protein subunit 1, 80 kDa nuclear cap-binding protein, NCBP 80 kDa subunit
Recommended products
Recombinant Human CBP80 protein is a Human Full Length protein, in the 1 to 790 aa range, expressed in Wheat germ and suitable for SDS-PAGE, ELISA, WB.
Key facts
- Expression system
- Wheat germ
- Tags
- Tag free
- Applications
- SDS-PAGE, ELISA, WB
- Biologically active
- No
- Accession
- Q09161-1
- Animal free
- No
- Species
- Human
- Concentration
- Storage buffer
pH: 8
Constituents: 0.79% Tris HCl, 0.3% Glutathione
Sequence info
Amino acid sequence
- M S R R R H S D E N D G G Q P H K R R K T S D A N E T E D H L E S L I C K V G E K S A C S L E S N L E G L A G V L E A D L P N Y K S K I L R L L C T V A R L L P E K L T I Y T T L V G L L N A R N Y N F G G E F V E A M I R Q L K E S L K A N N Y N E A V Y L V R F L S D L V N C H V I A A P S M V A M F E N F V S V T Q E E D V P Q V R R D W Y V Y A F L S S L P W V G K E L Y E K K D A E M D R I F A N T E S Y L K R R Q K T H V P M L Q V W T A D K P H P Q E E Y L D C L W A Q I Q K L K K D R W Q E R H I L R P Y L A F D S I L C E A L Q H N L P P F T P P P H T E D S V Y P M P R V I F R M F D Y T D D P E G P V M P G S H S V E R F V I E E N L H C I I K S H W K E R K T C A A Q L V S Y P G K N K I P L N Y H I V E V I F A E L F Q L P A P P H I D V M Y T T L L I E L C K L Q P G S L P Q V L A Q A T E M L Y M R L D T M N T T C V D R F I N W F S H H L S N F Q F R W S W E D W S D C L S Q D P E S P K P K F V R E V L E K C M R L S Y H Q R I L D I V P P T F S A L C P A N P T C I Y K Y G D E S S N S L P G H S V A L C L A V A F K S K A T N D E I F S I L K D V P N P N Q D D D D D E G F S F N P L K I E V F V Q T L L H L A A K S F S H S F S A L A K F H E V F K T L A E S D E G K L H V L R V M F E V W R N H P Q M I A V L V D K M I R T Q I V D C A A V A N W I F S S E L S R D F T R L F V W E I L H S T I R K M N K H V L K I Q K E L E E A K E K L A R Q H K R R S D D D D R S S D R K D G V L E E Q I E R L Q E K V E S A Q S E Q K N L F L V I F Q R F I M I L T E H L V R C E T D G T S V L T P W Y K N C I E R L Q Q I F L Q H H Q I I Q Q Y M V T L E N L L F T A E L D P H I L A V F Q Q F C A L Q A
- Accession
- Q09161
- Protein length
- Full Length
- Predicted molecular weight
- 112.64 kDa
- Amino acids
- 1 to 790
- Nature
- Recombinant
Specifications
- Form
- Liquid
General info
- Function
Component of the cap-binding complex (CBC), which binds cotranscriptionally to the 5'-cap of pre-mRNAs and is involved in various processes such as pre-mRNA splicing, translation regulation, nonsense-mediated mRNA decay, RNA-mediated gene silencing (RNAi) by microRNAs (miRNAs) and mRNA export. The CBC complex is involved in mRNA export from the nucleus via its interaction with ALYREF/THOC4/ALY, leading to the recruitment of the mRNA export machinery to the 5'-end of mRNA and to mRNA export in a 5' to 3' direction through the nuclear pore. The CBC complex is also involved in mediating U snRNA and intronless mRNAs export from the nucleus. The CBC complex is essential for a pioneer round of mRNA translation, before steady state translation when the CBC complex is replaced by cytoplasmic cap-binding protein eIF4E. The pioneer round of mRNA translation mediated by the CBC complex plays a central role in nonsense-mediated mRNA decay (NMD), NMD only taking place in mRNAs bound to the CBC complex, but not on eIF4E-bound mRNAs. The CBC complex enhances NMD in mRNAs containing at least one exon-junction complex (EJC) via its interaction with UPF1, promoting the interaction between UPF1 and UPF2. The CBC complex is also involved in 'failsafe' NMD, which is independent of the EJC complex, while it does not participate in Staufen-mediated mRNA decay (SMD). During cell proliferation, the CBC complex is also involved in microRNAs (miRNAs) biogenesis via its interaction with SRRT/ARS2 and is required for miRNA-mediated RNA interference. The CBC complex also acts as a negative regulator of PARN, thereby acting as an inhibitor of mRNA deadenylation. In the CBC complex, NCBP1/CBP80 does not bind directly capped RNAs (m7GpppG-capped RNA) but is required to stabilize the movement of the N-terminal loop of NCBP2/CBP20 and lock the CBC into a high affinity cap-binding state with the cap structure. Associates with NCBP3 to form an alternative cap-binding complex (CBC) which plays a key role in mRNA export and is particularly important in cellular stress situations such as virus infections. The conventional CBC with NCBP2 binds both small nuclear RNA (snRNA) and messenger (mRNA) and is involved in their export from the nucleus whereas the alternative CBC with NCBP3 does not bind snRNA and associates only with mRNA thereby playing a role only in mRNA export. NCBP1/CBP80 is required for cell growth and viability (PubMed:26382858).
- Sequence similarities
Belongs to the NCBP1 family.
- Subcellular localisation
- Nucleus
Storage
- Shipped at conditions
- Dry Ice
- Appropriate short-term storage conditions
- -80°C
- Appropriate long-term storage conditions
- -80°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
CBP80 also known as cap-binding protein 80 is an important component of the cap-binding complex (CBC) with a mass of about 80 kDa. This protein primarily binds to the 5' cap structure of newly synthesized pre-mRNA in eukaryotic cells. CBP80 often associates with CBP20 and together they form the CBC. It facilitates the processing of pre-mRNA and is mainly expressed in the nucleus of cells. CBP80 is integral in stabilizing RNA transcripts during their maturation.
- Biological function summary
CBP80 plays a role in the initiation of mRNA processing events. As a part of the CBC it influences RNA splicing export and translation initiation. It participates in early mRNA export from the nucleus to the cytoplasm and is essential in quality control mechanisms that ensure accurate mRNA translation. The CBP80-CBP20 complex also impacts nonsense-mediated mRNA decay (NMD) a process that degrades faulty mRNAs preventing the production of truncated proteins.
- Pathways
CBP80 integrates into the mRNA surveillance pathway where it works closely with other key proteins such as UPF1 UPF2 and UPF3 in the NMD pathway. These interactions ensure erroneous mRNA is efficiently targeted and degraded. Additionally CBP80 plays a minor role in the pathway of general mRNA export where it cooperates with export factors like CRM1. Its involvement in these pathways highlights its role in maintaining the fidelity of gene expression.
- Associated diseases and disorders
CBP80's malfunction has been linked to certain cancer types and neurodevelopmental disorders. Altered activity of CBP80 can lead to the accumulation of aberrant mRNA transcripts which contributes to oncogenesis. In some neurodevelopmental disorders mutations affecting CBP80 or its interacting partner CBP20 can disrupt normal neural development. This suggests that CBP80 not only maintains normal cell function but also prevents the development of disease through its regulation of gene expression.
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1 product image
SDS-PAGE - Recombinant Human CBP80 protein (ab114735)
12.5% SDS-PAGE showing ab114735 at approximately 112.64kDa.
Stained with Coomassie Blue.
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