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AB101110

Recombinant Human CBR4 protein (His tag N-Terminus)

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Recombinant Human CBR4 protein (His tag N-Terminus) is a Human Full Length protein, in the 1 to 237 aa range, expressed in Escherichia coli, with >95%, suitable for SDS-PAGE, WB, Mass Spec.

View Alternative Names

SDR45C1, CBR4, 3-oxoacyl-[acyl-carrier-protein] reductase, 3-ketoacyl-[acyl-carrier-protein] reductase beta subunit, Carbonyl reductase family member 4, Quinone reductase CBR4, Short chain dehydrogenase/reductase family 45C member 1, KAR beta subunit

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SDS-PAGE - Recombinant Human CBR4 protein (His tag N-Terminus) (AB101110)
  • SDS-PAGE

Unknown

SDS-PAGE - Recombinant Human CBR4 protein (His tag N-Terminus) (AB101110)

15% SDS-PAGE analysis of 3μg ab101110.

Key facts

Purity

>95% SDS-PAGE

Expression system

Escherichia coli

Tags

His tag N-Terminus

Applications

Mass Spec, SDS-PAGE, WB

applications

Biologically active

No

Accession

Q8N4T8

Animal free

No

Carrier free

No

Species

Human

Storage buffer

pH: 8 Constituents: 10% Glycerol (glycerin, glycerine), 1.16% Sodium chloride, 0.316% Tris HCl, 0.077% (R*,R*)-1,4-Dimercaptobutan-2,3-diol

storage-buffer

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "WB": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "Mass Spec": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Sequence info

[{"sequence":"MGSSHHHHHHSSGLVPRGSHMDKVCAVFGGSRGIGRAVAQLMARKGYRLAVIARNLEGAKAAAGDLGGDHLAFSCDVAKEHDVQNTFEEMEKHLGRVNFLVNAAGINRDGLLVRTKTEDMVSQLHTNLLGSMLTCKAAMRTMIQQQGGSIVNVGSIVGLKGNSGQSVYSASKGGLVGFSRALAKEVARKKIRVNVVAPGFVHTDMTKDLKEEHLKKNIPLGRFGETIEVAHAVVFLLESPYITGHVLVVDGGLQLIL","proteinLength":"Full Length","predictedMolecularWeight":"27.5 kDa","actualMolecularWeight":null,"aminoAcidEnd":237,"aminoAcidStart":1,"nature":"Recombinant","expressionSystem":"Escherichia coli","accessionNumber":"Q8N4T8","tags":[{"tag":"His","terminus":"N-Terminus"}]}]
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
False
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The CBR4 protein also known as Carbonyl Reductase 4 is an enzyme with a molecular mass of approximately 28 kDa. It functions as a NADPH-dependent oxidoreductase and plays a role in fatty acid metabolism. CBR4 is expressed in various tissues including the liver and the heart where it participates in several metabolic processes. The alternate name 'CBR4 SDS' is not typically used in scientific literature.
Biological function summary

CBR4 acts in concert with other proteins as part of a heterotetrameric complex. Its primary role involves the reduction of carbonyl compounds which is essential in lipid synthesis and energy production. The enzyme contributes to the maintenance of cellular redox states and is involved in mitochondrial function thereby supporting the energy demands of cells.

Pathways

The protein CBR4 integrates into the fatty acid biosynthetic and oxidative phosphorylation pathways. It processes carbonyl substrates in conjunction with other enzymes such as NADPH:quinone oxidoreductase. By facilitating these pathways CBR4 supports the cellular energy economy and the construction of cell membranes. Its activity interlinks with other mitochondrial enzymes enhancing the efficiency of mitochondrial respiration and lipid metabolism.

The CBR4 protein associates with disorders like non-alcoholic fatty liver disease and type 2 diabetes mellitus. The functional impairment of CBR4 may disrupt lipid metabolism leading to lipid accumulation and insulin resistance. CBR4's activity intersects with proteins like sterol regulatory element-binding proteins further connecting its role to metabolic syndrome and liver dysfunction.
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Specifications

Form

Liquid

Additional notes

ab101110 was purified using conventional chromatography techniques.

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General info

Function

Component of the heterotetramer complex KAR (3-ketoacyl-[acyl carrier protein] reductase or 3-ketoacyl-[ACP] reductase) that forms part of the mitochondrial fatty acid synthase (mtFAS). Beta-subunit of the KAR heterotetramer complex, responsible for the 3-ketoacyl-ACP reductase activity of the mtFAS, reduces 3-oxoacyl-[ACP] to (3R)-hydroxyacyl-[ACP] in a NADPH-dependent manner with no chain length preference, thereby participating in mitochondrial fatty acid biosynthesis (PubMed : 25203508). The homotetramer has NADPH-dependent quinone reductase activity (in vitro), hence could play a role in protection against cytotoxicity of exogenous quinones (PubMed : 19000905). As a heterotetramer, it can also reduce 9,10-phenanthrenequinone, 1,4-benzoquinone and various other o-quinones and p-quinones (in vitro) (PubMed : 19000905, PubMed : 19571038, PubMed : 25203508).

Sequence similarities

Belongs to the short-chain dehydrogenases/reductases (SDR) family.

Subcellular localisation

Mitochondrion matrix

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Product protocols

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Target data

Component of the heterotetramer complex KAR (3-ketoacyl-[acyl carrier protein] reductase or 3-ketoacyl-[ACP] reductase) that forms part of the mitochondrial fatty acid synthase (mtFAS). Beta-subunit of the KAR heterotetramer complex, responsible for the 3-ketoacyl-ACP reductase activity of the mtFAS, reduces 3-oxoacyl-[ACP] to (3R)-hydroxyacyl-[ACP] in a NADPH-dependent manner with no chain length preference, thereby participating in mitochondrial fatty acid biosynthesis (PubMed : 25203508). The homotetramer has NADPH-dependent quinone reductase activity (in vitro), hence could play a role in protection against cytotoxicity of exogenous quinones (PubMed : 19000905). As a heterotetramer, it can also reduce 9,10-phenanthrenequinone, 1,4-benzoquinone and various other o-quinones and p-quinones (in vitro) (PubMed : 19000905, PubMed : 19571038, PubMed : 25203508).
See full target information CBR4
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