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Recombinant Human CBX2 protein is a Human Fragment protein, in the 2 to 90 aa range, expressed in Escherichia coli, with >=90% purity and suitable for SDS-PAGE.

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Images

SDS-PAGE - Recombinant Human CBX2 protein (AB198759), expandable thumbnail

Key facts

Purity

>=90% SDS-PAGE

Expression system

Escherichia coli

Tags

His tag N-Terminus

Applications

SDS-PAGE

Biologically active

No

Amino acid sequence

M H H H H H H E E L S S V G E Q V F A A E C I L S K R L R K G K L E Y L V K W R G W S S K H N S W E P E E N I L D P R L L L A F Q K K E H E K E V Q N R K R G K R P R G R P R K L T A M S S C S

Reactivity data

Application

SDS-PAGE

Reactivity

Reacts

Dilution info

-

Notes

-

Associated Products

Select an associated product type

2 products for Alternative Product

Target data

Function

Component of a Polycomb group (PcG) multiprotein PRC1-like complex, a complex class required to maintain the transcriptionally repressive state of many genes, including Hox genes, throughout development (PubMed:21282530). PcG PRC1 complex acts via chromatin remodeling and modification of histones; it mediates monoubiquitination of histone H2A 'Lys-119', rendering chromatin heritably changed in its expressibility (PubMed:21282530). Binds to histone H3 trimethylated at 'Lys-9' (H3K9me3) or at 'Lys-27' (H3K27me3) (By similarity). Plays a role in the lineage differentiation of the germ layers in embryonic development (By similarity). Involved in sexual development, acting as activator of NR5A1 expression (PubMed:19361780).

Alternative names

Recommended products

Recombinant Human CBX2 protein is a Human Fragment protein, in the 2 to 90 aa range, expressed in Escherichia coli, with >=90% purity and suitable for SDS-PAGE.

Key facts

Purity

>=90% SDS-PAGE

Expression system

Escherichia coli

Applications

SDS-PAGE

Accession
Q14781-1
Animal free

No

Species

Human

Concentration
Loading...
Storage buffer

pH: 8
Constituents: 20% Glycerol (glycerin, glycerine), 0.64% Sodium chloride, 0.63% Tris HCl, 0.04% Sorbitan monolaurate, ethoxylated, 0.02% Potassium chloride

Sequence info

Amino acid sequence

M H H H H H H E E L S S V G E Q V F A A E C I L S K R L R K G K L E Y L V K W R G W S S K H N S W E P E E N I L D P R L L L A F Q K K E H E K E V Q N R K R G K R P R G R P R K L T A M S S C S

Accession

Q14781

Protein length

Fragment

Predicted molecular weight

11.4 kDa

Amino acids

2 to 90

Nature

Recombinant

Tags

His tag N-Terminus

Specifications

Form

Liquid

General info

Function

Component of a Polycomb group (PcG) multiprotein PRC1-like complex, a complex class required to maintain the transcriptionally repressive state of many genes, including Hox genes, throughout development (PubMed:21282530). PcG PRC1 complex acts via chromatin remodeling and modification of histones; it mediates monoubiquitination of histone H2A 'Lys-119', rendering chromatin heritably changed in its expressibility (PubMed:21282530). Binds to histone H3 trimethylated at 'Lys-9' (H3K9me3) or at 'Lys-27' (H3K27me3) (By similarity). Plays a role in the lineage differentiation of the germ layers in embryonic development (By similarity). Involved in sexual development, acting as activator of NR5A1 expression (PubMed:19361780).

Subcellular localisation

Nucleus

Storage

Shipped at conditions

Dry Ice

Appropriate short-term storage conditions

-80°C

Appropriate long-term storage conditions

-80°C

Storage information

Avoid freeze / thaw cycle

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.

Activity summary

The 'CBX2' protein also known as chromobox homolog 2 is an important component of the genetic regulation machinery. It weighs approximately 64 kDa and is mainly expressed in the nucleus of cells. CBX2 belongs to the Polycomb group (PcG) proteins specifically forming a part of the Polycomb repressive complex 1 (PRC1). Through its interaction with chromatin CBX2 induces transcriptional repression an important function for maintaining cellular identity and pluripotency.

Biological function summary

The effects of this protein extend to important roles in embryogenesis and cell cycle regulation. CBX2 serves as a part of PRC1 where it acts to mark chromatin for activation or repression. This complex allows CBX2 to recruit additional enzymes and proteins that modify chromatin structure ensuring tight regulation of gene expression. Cell differentiation depends heavily on the epigenetic control mediated by such proteins highlighting the importance of CBX2 in developmental biology.

Pathways

CBX2 plays integral roles in both the Wnt and the Hedgehog signaling pathways impacting cellular growth and differentiation. In these pathways CBX2 interacts with proteins like β-catenin in the Wnt pathway and GLI proteins in the Hedgehog pathway influencing gene transcription. These interactions highlight CBX2’s importance in switching genetic programs on or off in response to cellular signaling.

Associated diseases and disorders

Researchers have associated CBX2 with cancer and sexual development disorders. Abnormal expression patterns of CBX2 are often found in various cancer types where it can interact with proteins like BMI1 to alter chromatin states. Additionally mutations in CBX2 have been linked to disorders of sex development (DSD) where it influences genes critical for sexual differentiation. Understanding CBX2’s role could cast light on pathologies related to these diseases and provide potential therapeutic targets.

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1 product image

  • SDS-PAGE - Recombinant Human CBX2 protein (ab198759), expandable thumbnail

    SDS-PAGE - Recombinant Human CBX2 protein (ab198759)

    4-20% SDS-PAGE analysis of 2 μg ab198759 with Coomassie staining.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

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