JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB152885

Recombinant Human CBX4 protein

Be the first to review this product! Submit a review

|

(0 Publication)

Recombinant Human CBX4 protein is a Human Full Length protein, in the 1 to 290 aa range, expressed in Wheat germ, suitable for SDS-PAGE, ELISA, WB.

View Alternative Names

E3 SUMO-protein ligase CBX4, Chromobox protein homolog 4, Polycomb 2 homolog, Pc2, hPc2, CBX4

1 Images
SDS-PAGE - Recombinant Human CBX4 protein (AB152885)
  • SDS-PAGE

Unknown

SDS-PAGE - Recombinant Human CBX4 protein (AB152885)

12.5% SDS-PAGE analysis of ab152885 stained with Coomassie Blue.

Key facts

Expression system

Wheat germ

Tags

Tag free

Applications

SDS-PAGE, WB, ELISA

applications

Biologically active

No

Accession

O00257

Animal free

No

Carrier free

No

Species

Human

Storage buffer

pH: 8 Constituents: 0.79% Tris HCl, 0.31% Glutathione

storage-buffer

style
left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "ELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "WB": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
style
left-column
style
left-column

Sequence info

[{"sequence":"MELPAVGEHVFAVESIEKKRIRKGRVEYLVKWRGWSPKYNTWEPEENILDPRLLIAFQNRERQEQLMGYRKRGPKPKPLVVQVPTFARRSNVLTGLQDSSTDNRAKLDLGAQGKGQGHQYELNSKKHHHHAVGLNLSHVRKRCLSETHGEREPCKKRLTARSISTPTCLGGSPAAERPADLPPAAALPQPEVILLDSDLDEPIDLRCVKTRSEAGEPPSSLQVKPETPASAAVAVAAAAAPTTTAEKPPAEAQDEPAESLSEFKPFFGNIIITDVTANCLTVTFKEYVTV","proteinLength":"Full Length","predictedMolecularWeight":"58.4 kDa","actualMolecularWeight":null,"aminoAcidEnd":290,"aminoAcidStart":1,"nature":"Recombinant","expressionSystem":"Wheat germ","accessionNumber":"O00257","tags":[]}]
style
left-column

Properties and storage information

Shipped at conditions
Dry Ice
Appropriate long-term storage conditions
-80°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
False
style
left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

CBX4 also known as Chromobox protein homolog 4 or Polycomb 2 acts mechanically as part of the Polycomb group (PcG) proteins. It weighs approximately 60 kDa and it is found in nuclear matrix where it participates in chromatin remodeling. CBX4 shows significant expression in tissues like liver brain and lung indicating its wide importance to cellular function. By binding to methylated histones especially H3K27me3 CBX4 influences gene silencing and epigenetic regulation important for maintaining cellular identity.
Biological function summary

CBX4 operates as a component of the Polycomb Repressive Complex 1 (PRC1) which plays role in controlling gene expression patterns during development. In this complex CBX4 interacts with other core components such as BMI1 and RING1 to facilitate monoubiquitination of histone H2A leading to transcriptional repression. This ability of CBX4 to modulate epigenetic states highlights its importance in critical developmental processes and differentiation.

Pathways

CBX4 significantly influences gene regulatory networks involved in cell cycle and apoptosis pathways. In the cell cycle regulation CBX4 interacts with important proteins such as Cyclin D1 impacting cell proliferation. In apoptosis CBX4 has roles linked to the p53 pathway by modulating transcriptional activity which influences cellular fate. These pathways underline the significance of CBX4 in maintaining balance between cell growth and death.

The deregulation of CBX4 expression has been associated with cancers like hepatocellular carcinoma and breast cancer. CBX4 potentially interacts with proteins such as p21 and p16 whose pathways play significant roles in cancer development. This underlines its role as a potential biomarker or therapeutic target in cancer treatment strategies. Understanding the function and regulation of CBX4 could offer insights in devising novel treatments for these malignancies.
style
left-column

Specifications

Form

Liquid

style
left-column

General info

Function

E3 SUMO-protein ligase which facilitates SUMO1 conjugation by UBE2I (PubMed : 12679040). Involved in the sumoylation of HNRNPK, a p53/TP53 transcriptional coactivator, hence indirectly regulates p53/TP53 transcriptional activation resulting in p21/CDKN1A expression. Monosumoylates ZNF131 (PubMed : 22825850).. Component of a Polycomb group (PcG) multiprotein PRC1-like complex, a complex class required to maintain the transcriptionally repressive state of many genes, including Hox genes, throughout development (PubMed : 12167701, PubMed : 19636380, PubMed : 21282530). PcG PRC1 complex acts via chromatin remodeling and modification of histones; it mediates monoubiquitination of histone H2A 'Lys-119', rendering chromatin heritably changed in its expressibility (PubMed : 12167701, PubMed : 19636380, PubMed : 21282530). Binds to histone H3 trimethylated at 'Lys-9' (H3K9me3) (By similarity). Plays a role in the lineage differentiation of the germ layers in embryonic development (By similarity).

Post-translational modifications

Ubiquitinated. Ubiquitination regulates the function of the Polycomb group (PcG) multiprotein PRC1-like complex. Deubiquitinated by USP26.. Phosphorylated on Thr-497 by HIPK2 upon DNA damage. This phosphorylation stimulates E3 SUMO-protein ligase activity and promotes sumoylation on Lys-494, as well as sumoylation of other target proteins, such as HNRNPK.

Subcellular localisation

Nucleus

style
left-column

Product protocols

style
left-column

Target data

E3 SUMO-protein ligase which facilitates SUMO1 conjugation by UBE2I (PubMed : 12679040). Involved in the sumoylation of HNRNPK, a p53/TP53 transcriptional coactivator, hence indirectly regulates p53/TP53 transcriptional activation resulting in p21/CDKN1A expression. Monosumoylates ZNF131 (PubMed : 22825850).. Component of a Polycomb group (PcG) multiprotein PRC1-like complex, a complex class required to maintain the transcriptionally repressive state of many genes, including Hox genes, throughout development (PubMed : 12167701, PubMed : 19636380, PubMed : 21282530). PcG PRC1 complex acts via chromatin remodeling and modification of histones; it mediates monoubiquitination of histone H2A 'Lys-119', rendering chromatin heritably changed in its expressibility (PubMed : 12167701, PubMed : 19636380, PubMed : 21282530). Binds to histone H3 trimethylated at 'Lys-9' (H3K9me3) (By similarity). Plays a role in the lineage differentiation of the germ layers in embryonic development (By similarity).
See full target information CBX4
style
left-column
style
left-column
style
right-column

Complementary Products

Primary Antibodies

AB242149

Anti-CBX4 antibody [EPR23053-7] - ChIP Grade

BOND RX™ Validated|RabMAb|Recombinant

ChIP - Anti-CBX4 antibody [EPR23053-7] - ChIP Grade (AB242149)

  • 4
  • 1
Cell Lines & Lysates

AB261723

Human CBX4 knockout HEK-293 cell line

Western blot - Human CBX4 knockout HEK-293 cell line (AB261723)

  • 0
  • 0
Primary Antibodies

AB109453

Anti-p95/NBS1 (phospho S343) antibody [EP178]

RabMAb|Recombinant

Western blot - Anti-p95/NBS1 (phospho S343) antibody [EP178] (AB109453)

  • 0
  • 0
Primary Antibodies

AB252269

Anti-TCF12 antibody [7B3] - BSA and Azide free

Recombinant

Western blot - Anti-TCF12 antibody [7B3] - BSA and Azide free (AB252269)

  • 0
  • 0

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com