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Recombinant Human CCK4 protein (His tag) is a Human Fragment protein, in the 31 to 704 aa range, expressed in Yeast, with >90% purity and suitable for SDS-PAGE, MS.

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Images

SDS-PAGE - Recombinant Human CCK4 protein (His tag) (AB241239), expandable thumbnail
  • Mass Spectrometry - Recombinant Human CCK4 protein (His tag) (AB241239), expandable thumbnail
  • Mass Spectrometry - Recombinant Human CCK4 protein (His tag) (AB241239), expandable thumbnail

Key facts

Purity

>90% SDS-PAGE

Expression system

Yeast

Tags

His tag N-Terminus

Applications

SDS-PAGE, MS

Biologically active

No

Amino acid sequence

A I V F I K Q P S S Q D A L Q G R R A L L R C E V E A P G P V H V Y W L L D G A P V Q D T E R R F A Q G S S L S F A A V D R L Q D S G T F Q C V A R D D V T G E E A R S A N A S F N I K W I E A G P V V L K H P A S E A E I Q P Q T Q V T L R C H I D G H P R P T Y Q W F R D G T P L S D G Q S N H T V S S K E R N L T L R P A G P E H S G L Y S C C A H S A F G Q A C S S Q N F T L S I A D E S F A R V V L A P Q D V V V A R Y E E A M F H C Q F S A Q P P P S L Q W L F E D E T P I T N R S R P P H L R R A T V F A N G S L L L T Q V R P R N A G I Y R C I G Q G Q R G P P I I L E A T L H L A E I E D M P L F E P R V F T A G S E E R V T C L P P K G L P E P S V W W E H A G V R L P T H G R V Y Q K G H E L V L A N I A E S D A G V Y T C H A A N L A G Q R R Q D V N I T V A T V P S W L K K P Q D S Q L E E G K P G Y L D C L T Q A T P K P T V V W Y R N Q M L I S E D S R F E V F K N G T L R I N S V E V Y D G T W Y R C M S S T P A G S I E A Q A R V Q V L E K L K F T P P P Q P Q Q C M E F D K E A T V P C S A T G R E K P T I K W E R A D G S S L P E W V T D N A G T L H F A R V T R D D A G N Y T C I A S N G P Q G Q I R A H V Q L T V A V F I T F K V E P E R T T V Y Q G H T A L L Q C E A Q G D P K P L I Q W K G K D R I L D P T K L G P R M H I F Q N G S L V I H D V A P E D S G R Y T C I A G N S C N I K H T E A P L Y V V D K P V P E E S E G P G S P P P Y K M I Q T

Reactivity data

Application

SDS-PAGE

Reactivity

Reacts

Dilution info

-

Notes

-

Application

MS

Reactivity

Reacts

Dilution info

-

Notes

-

Target data

Function

Inactive tyrosine kinase involved in Wnt signaling pathway. Component of both the non-canonical (also known as the Wnt/planar cell polarity signaling) and the canonical Wnt signaling pathway. Functions in cell adhesion, cell migration, cell polarity, proliferation, actin cytoskeleton reorganization and apoptosis. Has a role in embryogenesis, epithelial tissue organization and angiogenesis.

Alternative names

Recommended products

Recombinant Human CCK4 protein (His tag) is a Human Fragment protein, in the 31 to 704 aa range, expressed in Yeast, with >90% purity and suitable for SDS-PAGE, MS.

Key facts

Purity

>90% SDS-PAGE

Expression system

Yeast

Applications

SDS-PAGE, MS

Accession
Q13308-1
Animal free

No

Species

Human

Concentration
Loading...
Storage buffer

pH: 7.2 - 7.4
Constituents: Tris buffer, 50% Glycerol (glycerin, glycerine)

Sequence info

Amino acid sequence

A I V F I K Q P S S Q D A L Q G R R A L L R C E V E A P G P V H V Y W L L D G A P V Q D T E R R F A Q G S S L S F A A V D R L Q D S G T F Q C V A R D D V T G E E A R S A N A S F N I K W I E A G P V V L K H P A S E A E I Q P Q T Q V T L R C H I D G H P R P T Y Q W F R D G T P L S D G Q S N H T V S S K E R N L T L R P A G P E H S G L Y S C C A H S A F G Q A C S S Q N F T L S I A D E S F A R V V L A P Q D V V V A R Y E E A M F H C Q F S A Q P P P S L Q W L F E D E T P I T N R S R P P H L R R A T V F A N G S L L L T Q V R P R N A G I Y R C I G Q G Q R G P P I I L E A T L H L A E I E D M P L F E P R V F T A G S E E R V T C L P P K G L P E P S V W W E H A G V R L P T H G R V Y Q K G H E L V L A N I A E S D A G V Y T C H A A N L A G Q R R Q D V N I T V A T V P S W L K K P Q D S Q L E E G K P G Y L D C L T Q A T P K P T V V W Y R N Q M L I S E D S R F E V F K N G T L R I N S V E V Y D G T W Y R C M S S T P A G S I E A Q A R V Q V L E K L K F T P P P Q P Q Q C M E F D K E A T V P C S A T G R E K P T I K W E R A D G S S L P E W V T D N A G T L H F A R V T R D D A G N Y T C I A S N G P Q G Q I R A H V Q L T V A V F I T F K V E P E R T T V Y Q G H T A L L Q C E A Q G D P K P L I Q W K G K D R I L D P T K L G P R M H I F Q N G S L V I H D V A P E D S G R Y T C I A G N S C N I K H T E A P L Y V V D K P V P E E S E G P G S P P P Y K M I Q T

Accession

Q13308

Protein length

Fragment

Predicted molecular weight

76.6 kDa

Amino acids

31 to 704

Nature

Recombinant

Tags

His tag N-Terminus

Specifications

Form

Liquid

General info

Function

Inactive tyrosine kinase involved in Wnt signaling pathway. Component of both the non-canonical (also known as the Wnt/planar cell polarity signaling) and the canonical Wnt signaling pathway. Functions in cell adhesion, cell migration, cell polarity, proliferation, actin cytoskeleton reorganization and apoptosis. Has a role in embryogenesis, epithelial tissue organization and angiogenesis.

Sequence similarities

Belongs to the protein kinase superfamily. Tyr protein kinase family. Insulin receptor subfamily.

Post-translational modifications

MMP14 cleaves PTK7 between Pro-621 and Leu-622 generating an N-terminal soluble (70 kDa) fragment and a membrane C-terminal (50 kDa) fragment. Proteolysis by MMP14 regulates PTK7 function in non-canonical Wnt signaling pathway.

Storage

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

-20°C

Appropriate long-term storage conditions

-20°C

Storage information

Avoid freeze / thaw cycle

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.

Activity summary

CCK4 also known as PTK7 protein is a receptor tyrosine kinase. The molecular mass of the CCK4 protein is approximately 118 kDa. It is expressed in various tissues including the placenta lung kidney and colon. The presence of CCK4 is particularly noted in developmental tissues and has roles in embryogenesis. Despite its classification as a receptor tyrosine kinase CCK4 lacks catalytic activity indicating its unique functional properties.

Biological function summary

CCK4 participates in cellular signaling involved in adhesion and cell migration. It localizes predominantly in the membranes participating in complex cellular environments such as the Wnt signaling complex. This suggests a role in maintaining cellular architecture and signaling pathways that influence developmental processes. Its interactions within these complexes can help maintain balance and functional integrity in developing tissues.

Pathways

CCK4 engages in vital pathways influencing cell adhesion and movement including the planar cell polarity (PCP) pathway and Wnt signaling pathway. Within these pathways CCK4 interacts with proteins such as Dishevelled and Frizzled establishing its role in guiding cellular and developmental patterns. These interactions regulate important aspects of cell polarity and directional movement during embryonic development.

Associated diseases and disorders

CCK4 has been implicated in cancer and congenital malformations. In cancers such as colorectal carcinoma altered CCK4 expression correlates with cancer progression possibly through interaction with associated proteins like ROR2. Similarly its function in neural tube development suggests a role in congenital disorders affecting the spine and nervous system. This connection highlights the importance of CCK4 in both cancer biology and developmental disorders.

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3 product images

  • SDS-PAGE - Recombinant Human CCK4 protein (His tag) (ab241239), expandable thumbnail

    SDS-PAGE - Recombinant Human CCK4 protein (His tag) (ab241239)

    (Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel analysis of ab241239.

  • Mass Spectrometry - Recombinant Human CCK4 protein (His tag) (ab241239), expandable thumbnail

    Mass Spectrometry - Recombinant Human CCK4 protein (His tag) (ab241239)

    Based on the SEQUEST from database of Yeast host and target protein, the LC-MS/MS Analysis result of ab241239 could indicate that this peptide derived from Yeast-expressed Homo sapiens (Human) CCK4.

  • Mass Spectrometry - Recombinant Human CCK4 protein (His tag) (ab241239), expandable thumbnail

    Mass Spectrometry - Recombinant Human CCK4 protein (His tag) (ab241239)

    Based on the SEQUEST from database of Yeast host and target protein, the LC-MS/MS Analysis result of ab241239 could indicate that this peptide derived from Yeast-expressed Homo sapiens (Human) CCK4.

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Product protocols

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