Recombinant Human CCK4 protein (Tagged) is a Human Fragment protein, in the 726 to 1070 aa range, expressed in Baculovirus infected Sf9, with >95% purity and suitable for SDS-PAGE.
Images
Key facts
- Purity
- >95% SDS-PAGE
- Expression system
- Baculovirus infected Sf9 cells
- Tags
- GST tag N-Terminus
- Applications
- SDS-PAGE
- Biologically active
- No
Amino acid sequence
C K K R C K A K R L Q K Q P E G E E P E M E C L N G G P L Q N G Q P S A E I Q E E V A L T S L G S G P A A T N K R H S T S D K M H F P R S S L Q P I T T L G K S E F G E V F L A K A Q G L E E G V A E T L V L V K S L Q S K D E Q Q Q L D F R R E L E M F G K L N H A N V V R L L G L C R E A E P H Y M V L E Y V D L G D L K Q F L R I S K S K D E K L K S Q P L S T K Q K V A L C T Q V A L G M E H L S N N R F V H K D L A A R N C L V S A Q R Q V K V S A L G L S K D V Y N S E Y Y H F R Q A W V P L R W M S P E A I L E G D F S T K S D V W A F G V L M W E V F T H G E M P H G G Q A D D E V L A D L Q A G K A R L P Q P E G C P S K L Y R L M Q R C W A L S P K D R P S F S E I A S A L G D S T V D S K P
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application SDS-PAGE | Reactivity Reacts | Dilution info - | Notes - |
Target data
Function
Inactive tyrosine kinase involved in Wnt signaling pathway. Component of both the non-canonical (also known as the Wnt/planar cell polarity signaling) and the canonical Wnt signaling pathway. Functions in cell adhesion, cell migration, cell polarity, proliferation, actin cytoskeleton reorganization and apoptosis. Has a role in embryogenesis, epithelial tissue organization and angiogenesis.
Alternative names
CCK4, PTK7, Inactive tyrosine-protein kinase 7, Colon carcinoma kinase 4, Protein-tyrosine kinase 7, Pseudo tyrosine kinase receptor 7, Tyrosine-protein kinase-like 7, CCK-4
Recommended products
Recombinant Human CCK4 protein (Tagged) is a Human Fragment protein, in the 726 to 1070 aa range, expressed in Baculovirus infected Sf9, with >95% purity and suitable for SDS-PAGE.
Key facts
- Purity
- >95% SDS-PAGE
- Expression system
- Baculovirus infected Sf9 cells
- Tags
- GST tag N-Terminus
- Applications
- SDS-PAGE
- Biologically active
- No
- Accession
- Q13308-1
- Animal free
- No
- Species
- Human
- Concentration
- Storage buffer
pH: 7.5
Constituents: 25% Glycerol (glycerin, glycerine), 0.79% Tris HCl, 0.31% Glutathione, 0.29% Sodium chloride, 0.004% (R*,R*)-1,4-Dimercaptobutan-2,3-diol, 0.003% EDTA, 0.002% PMSF
Sequence info
Amino acid sequence
- C K K R C K A K R L Q K Q P E G E E P E M E C L N G G P L Q N G Q P S A E I Q E E V A L T S L G S G P A A T N K R H S T S D K M H F P R S S L Q P I T T L G K S E F G E V F L A K A Q G L E E G V A E T L V L V K S L Q S K D E Q Q Q L D F R R E L E M F G K L N H A N V V R L L G L C R E A E P H Y M V L E Y V D L G D L K Q F L R I S K S K D E K L K S Q P L S T K Q K V A L C T Q V A L G M E H L S N N R F V H K D L A A R N C L V S A Q R Q V K V S A L G L S K D V Y N S E Y Y H F R Q A W V P L R W M S P E A I L E G D F S T K S D V W A F G V L M W E V F T H G E M P H G G Q A D D E V L A D L Q A G K A R L P Q P E G C P S K L Y R L M Q R C W A L S P K D R P S F S E I A S A L G D S T V D S K P
- Accession
- Q13308
- Protein length
- Fragment
- Amino acids
- 726 to 1070
- Nature
- Recombinant
- Tags
- GST tag N-Terminus
Specifications
- Form
- Liquid
- Additional notes
Affinity purified.
General info
- Function
Inactive tyrosine kinase involved in Wnt signaling pathway. Component of both the non-canonical (also known as the Wnt/planar cell polarity signaling) and the canonical Wnt signaling pathway. Functions in cell adhesion, cell migration, cell polarity, proliferation, actin cytoskeleton reorganization and apoptosis. Has a role in embryogenesis, epithelial tissue organization and angiogenesis.
- Sequence similarities
Belongs to the protein kinase superfamily. Tyr protein kinase family. Insulin receptor subfamily.
- Post-translational modifications
MMP14 cleaves PTK7 between Pro-621 and Leu-622 generating an N-terminal soluble (70 kDa) fragment and a membrane C-terminal (50 kDa) fragment. Proteolysis by MMP14 regulates PTK7 function in non-canonical Wnt signaling pathway.
Storage
- Shipped at conditions
- Dry Ice
- Appropriate short-term storage conditions
- -80°C
- Appropriate long-term storage conditions
- -80°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
CCK4 also known as PTK7 protein is a receptor tyrosine kinase. The molecular mass of the CCK4 protein is approximately 118 kDa. It is expressed in various tissues including the placenta lung kidney and colon. The presence of CCK4 is particularly noted in developmental tissues and has roles in embryogenesis. Despite its classification as a receptor tyrosine kinase CCK4 lacks catalytic activity indicating its unique functional properties.
- Biological function summary
CCK4 participates in cellular signaling involved in adhesion and cell migration. It localizes predominantly in the membranes participating in complex cellular environments such as the Wnt signaling complex. This suggests a role in maintaining cellular architecture and signaling pathways that influence developmental processes. Its interactions within these complexes can help maintain balance and functional integrity in developing tissues.
- Pathways
CCK4 engages in vital pathways influencing cell adhesion and movement including the planar cell polarity (PCP) pathway and Wnt signaling pathway. Within these pathways CCK4 interacts with proteins such as Dishevelled and Frizzled establishing its role in guiding cellular and developmental patterns. These interactions regulate important aspects of cell polarity and directional movement during embryonic development.
- Associated diseases and disorders
CCK4 has been implicated in cancer and congenital malformations. In cancers such as colorectal carcinoma altered CCK4 expression correlates with cancer progression possibly through interaction with associated proteins like ROR2. Similarly its function in neural tube development suggests a role in congenital disorders affecting the spine and nervous system. This connection highlights the importance of CCK4 in both cancer biology and developmental disorders.
Product promise
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
1 product image
SDS-PAGE - Recombinant Human CCK4 protein (Tagged) (ab268895)
SDS-PAGE analysis of ab268895.
Downloads
Product protocols
- Visit the general protocols
- Visit troubleshooting
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
For licensing inquiries, please contact partnerships@abcam.com