Recombinant Human CCL1 protein is a Human Fragment protein, in the 24 to 96 aa range, expressed in HEK 293, with >=95% purity, <= 0.005 EU/µg endotoxin level and suitable for HPLC, MS.
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Key facts
- Purity
- >=95% HPLC
- Endotoxin level
- <= 0.005 EU/µg
- Expression system
- HEK 293 cells
- Tags
- Tag free
- Applications
- HPLC, MS
- Biologically active
- No
- Mass spectrometry
- LC-MS/MS
Amino acid sequence
K S M Q V P F S R C C F S F A E Q E I P L R A I L C Y R N T S S I C S N E G L I F K L K R G K E A C A L D T V G W V Q R H R K M L R H C P S K R K
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application HPLC | Reactivity Reacts | Dilution info - | Notes - |
Application MS | Reactivity Reacts | Dilution info - | Notes - |
Target data
Function
Cytokine that is chemotactic for monocytes but not for neutrophils. Binds to CCR8.
Alternative names
SCYA1, CCL1, C-C motif chemokine 1, Small-inducible cytokine A1, T lymphocyte-secreted protein I-309
Recommended products
Recombinant Human CCL1 protein is a Human Fragment protein, in the 24 to 96 aa range, expressed in HEK 293, with >=95% purity, <= 0.005 EU/µg endotoxin level and suitable for HPLC, MS.
Key facts
- Purity
- >=95% HPLC
- Endotoxin level
- <= 0.005 EU/µg
- Expression system
- HEK 293 cells
- Tags
- Tag free
- Applications
- HPLC, MS
- Biologically active
- No
- Mass spectrometry
- LC-MS/MS
- Accession
- P22362-1
- Animal free
- Yes
- Species
- Human
- Reconstitution
- Lyophilized contents may appear as either a translucent film or a white powder. This variance does not affect the quality of the product. Store lyophilized form at room temperature. Reconstitute in phosphate buffered saline, aliquot and store at -80°C for 12 months or +4°C for 1 week. Avoid repeated freeze-thaw.
- Concentration
- Storage buffer
pH: 7.4
Constituents: 10.26% Trehalose, 0.727% Dibasic monohydrogen potassium phosphate, 0.248% Potassium phosphate monobasic
Sequence info
Amino acid sequence
- K S M Q V P F S R C C F S F A E Q E I P L R A I L C Y R N T S S I C S N E G L I F K L K R G K E A C A L D T V G W V Q R H R K M L R H C P S K R K
- Accession
- P22362
- Protein length
- Fragment
- Predicted molecular weight
- 8.55 kDa
- Actual molecular weight
- 8.14 kDa
- Amino acids
- 24 to 96
- Nature
- Recombinant
Specifications
- Form
- Lyophilized
General info
- Function
Cytokine that is chemotactic for monocytes but not for neutrophils. Binds to CCR8.
- Sequence similarities
Belongs to the intercrine beta (chemokine CC) family.
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- Ambient
- Appropriate long-term storage conditions
- Ambient
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
The CCL1 protein also known as I-309 protein is a cytokine that plays a significant role in the immune system. Its molecular mass is approximately 10 kDa. CCL1 is secreted by activated T cells and is found extensively in the thymus. It binds to the CCR8 receptor which suggests its involvement in immune signaling. This protein belongs to the CC chemokine family which is characterized by the presence of two adjacent cysteines near the amino terminus.
- Biological function summary
CCL1 influences immune cell trafficking and activation. It attracts monocytes T lymphocytes and some types of dendritic cells to sites of inflammation. Although it is not part of a large protein complex CCL1 works with other chemokines to modulate the immune response contributing to both innate and adaptive immune mechanisms. Its expression may increase or decrease depending on the immune needs of the body.
- Pathways
Studies show that CCL1 participates in the chemokine signaling pathway and the effector T-cell pathway. Within the chemokine signaling pathway CCL1 interacts with other chemokines like CCL17 and proteins involved in T-cell migration. This pathway helps coordinate the movement of immune cells to sites of inflammation or injury ensuring an effective immune response.
- Associated diseases and disorders
Research links CCL1 to conditions such as asthma and rheumatoid arthritis. In asthma CCL1 levels influence the recruitment of immune cells to the lungs contributing to inflammation and airway hyper-responsiveness. Similarly in rheumatoid arthritis its interaction with CCR8 has been associated with synovial inflammation. These diseases indicate that CCL1’s role in immune cell migration can have pathological consequences when dysregulated.
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2 product images
Mass Spectrometry - Recombinant Human CCL1 protein (ab315436)
Mass determination by ESI-TOF.
Predicted MW is 8550 Da (+/- 10 Da by ESI-TOF). Observed MW is 8140 Da.
HPLC - Recombinant Human CCL1 protein (ab315436)
HPLC analysis of ab315436
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Product protocols
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