Recombinant Human CCL21 protein is a Human Full Length protein, in the 24 to 134 aa range, expressed in Escherichia coli, with >95% purity, <= 1 EU/µg endotoxin level and suitable for SDS-PAGE.
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Key facts
- Purity
>95% SDS-PAGE
- Endotoxin level
<= 1 EU/µg
- Expression system
Escherichia coli
- Tags
Tag free
- Applications
SDS-PAGE
- Biologically active
No
Amino acid sequence
S D G G A Q D C C L K Y S Q R K I P A K V V R S Y R K Q E P S L G C S I P A I L F L P R K R S Q A E L C A D P K E L W V Q Q L M Q H L D K T P S P Q K P A Q G C R K D R G A S K T G K K G K G S K G C K R T E R S Q T P K G P
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application SDS-PAGE | Reactivity Reacts | Dilution info - | Notes - |
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Target data
Function
Inhibits hemopoiesis and stimulates chemotaxis. Chemotactic in vitro for thymocytes and activated T-cells, but not for B-cells, macrophages, or neutrophils. Shows preferential activity towards naive T-cells. May play a role in mediating homing of lymphocytes to secondary lymphoid organs. Binds to atypical chemokine receptor ACKR4 and mediates the recruitment of beta-arrestin (ARRB1/2) to ACKR4.
Alternative names
C-C motif chemokine 21, 6Ckine, Beta-chemokine exodus-2, Secondary lymphoid-tissue chemokine, Small-inducible cytokine A21, SLC, UNQ784/PRO1600, CCL21, SCYA21
Recommended products
Recombinant Human CCL21 protein is a Human Full Length protein, in the 24 to 134 aa range, expressed in Escherichia coli, with >95% purity, <= 1 EU/µg endotoxin level and suitable for SDS-PAGE.
Alternative names
C-C motif chemokine 21, 6Ckine, Beta-chemokine exodus-2, Secondary lymphoid-tissue chemokine, Small-inducible cytokine A21, SLC, UNQ784/PRO1600, CCL21, SCYA21
Key facts
- Purity
>95% SDS-PAGE
- Endotoxin level
<= 1 EU/µg
- Expression system
Escherichia coli
- Tags
Tag free
- Applications
SDS-PAGE
- Biologically active
No
- Accession
- O00585-1
- Animal free
No
- Species
Human
- Reconstitution
Centrifuge vial before opening. Suspend the product by gently pipetting sterile deionized water down the sides of the vial to a final concentration of 0.1 mg/ml. DO NOT VORTEX. Allow several minutes for complete reconstitution. For prolonged storage, dilute to working aliquots in a 0.1% BSA solution, store at -80°C and avoid repeat freeze thaw.
- Concentration
- Storage buffer
Constituents: 0.1% Trifluoroacetic acid
Sequence info
Amino acid sequence
S D G G A Q D C C L K Y S Q R K I P A K V V R S Y R K Q E P S L G C S I P A I L F L P R K R S Q A E L C A D P K E L W V Q Q L M Q H L D K T P S P Q K P A Q G C R K D R G A S K T G K K G K G S K G C K R T E R S Q T P K G P
- Accession
- Protein length
Full Length
- Predicted molecular weight
12.3 kDa
- Amino acids
24 to 134
- Nature
Recombinant
Specifications
- Form
Lyophilized
General info
- Function
Inhibits hemopoiesis and stimulates chemotaxis. Chemotactic in vitro for thymocytes and activated T-cells, but not for B-cells, macrophages, or neutrophils. Shows preferential activity towards naive T-cells. May play a role in mediating homing of lymphocytes to secondary lymphoid organs. Binds to atypical chemokine receptor ACKR4 and mediates the recruitment of beta-arrestin (ARRB1/2) to ACKR4.
- Sequence similarities
Belongs to the intercrine beta (chemokine CC) family.
Storage
- Shipped at conditions
Blue Ice
- Appropriate long-term storage conditions
-20°C
- Storage information
Avoid freeze / thaw cycle
Supplementary info
This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
CCL21 also known as Exodus 2 or 2H26 is a chemokine with significant roles in the immune system. It is a small protein with a molecular mass of approximately 12 kDa. CCL21 is expressed in high concentrations in secondary lymphoid organs such as the spleen lymph nodes and Peyer's patches. It serves as a chemoattractant particularly for lymphocytes and dendritic cells facilitating their migration towards lymphoid organs.
- Biological function summary
CCL21 is essential for guiding immune cells to areas where they are needed for initiating immune responses. It forms a non-covalent complex with its receptor CCR7 a primary mechanism through which it exerts its biological effects. CCL21 coordinates the positioning of immune cells within the lymphoid organs ensuring a functional and effective immune response to pathogens or injuries. Its expression pattern aids in the organization of lymphoid tissue architecture and contributes to efficient trafficking of T-cells and dendritic cells.
- Pathways
CCL21 plays an important role in the chemokine signaling pathway and the leukocyte transendothelial migration pathway. Within these pathways CCL21 works closely with its receptor CCR7 to guide immune cell navigation and localization. This collaboration is essential for directing cells to appropriate sites in the immune system aligning CCL21 with similar proteins like CCL19 another chemokine that also interacts with CCR7.
- Associated diseases and disorders
CCL21 is associated with several immune-related conditions including rheumatoid arthritis and chronic lymphocytic leukemia. In rheumatoid arthritis CCL21 contributes to the infiltration and retention of immune cells in the synovial tissue while in chronic lymphocytic leukemia it aids in the homing of malignant cells to lymphoid organs. CCL21’s interaction with CCR7 in these diseases highlights its importance not only in immune surveillance but also in the pathology of these disorders.
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1 product image
SDS-PAGE - Recombinant Human CCL21 protein (ab222174)
1 μg ab222174 analyzed on a 4-20% Tris-Glycine gel, stained with Coomassie Blue.
Lane 1: Non-reducing conditions.
Lane 2: Reducing conditions.
ab222174 is predicted to have a molecuar weight of 12.3 kDa, but runs at about 16 kDa.
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