Recombinant Human CCL27 protein (Active) is a Human Fragment protein, in the 25 to 112 aa range, expressed in HEK 293, <= 0.005 EU/µg endotoxin level and suitable for SDS-PAGE, MS, HPLC.
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Key facts
- Purity
HPLC
- Endotoxin level
<= 0.005 EU/µg
- Expression system
HEK 293 cells
- Tags
Tag free
- Applications
SDS-PAGE, MS, HPLC
- Biologically active
Yes
Amino acid sequence
F L L P P S T A C C T Q L Y R K P L S D K L L R K V I Q V E L Q E A D G D C H L Q A F V L H L A Q R S I C I H P Q N P S L S Q W F E H Q E R K L H G T L P K L N F G M L R K M G
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application SDS-PAGE | Reactivity Reacts | Dilution info - | Notes - |
Application MS | Reactivity Reacts | Dilution info - | Notes - |
Application HPLC | Reactivity Reacts | Dilution info - | Notes - |
Target data
Function
Chemotactic factor that attracts skin-associated memory T-lymphocytes. May play a role in mediating homing of lymphocytes to cutaneous sites. Binds to CCR10.
Alternative names
ILC, SCYA27, CCL27, SCYA27, ILC, C-C motif chemokine 27, CC chemokine ILC, Cutaneous T-cell-attracting chemokine, ESkine, IL-11 R-alpha-locus chemokine, Skinkine, Small-inducible cytokine A27, CTACK
Recommended products
Recombinant Human CCL27 protein (Active) is a Human Fragment protein, in the 25 to 112 aa range, expressed in HEK 293, <= 0.005 EU/µg endotoxin level and suitable for SDS-PAGE, MS, HPLC.
Key facts
- Purity
HPLC
- Endotoxin level
<= 0.005 EU/µg
- Expression system
HEK 293 cells
- Tags
Tag free
- Applications
SDS-PAGE, MS, HPLC
- Biologically active
Yes
- Biological activity
Fully biologically active determined by the dose dependent measurement of beta-arrestin recruitment on CCR10 using U2OS overexpressing human CCR10/beta-arrestin/beta-galactosidase complementation system.
ED50 is ≤ 295 ng/ml, corresponding to a specific activity of 3.4 x 103 units/mg.
- Accession
- Q9Y4X3-1
- Animal free
Yes
- Species
Human
- Reconstitution
Reconstitute in PBS
- Concentration
- Storage buffer
pH: 7.4
Constituents: 10.26% Trehalose, 0.727% Dibasic monohydrogen potassium phosphate, 0.248% Potassium phosphate monobasic
Sequence info
Amino acid sequence
F L L P P S T A C C T Q L Y R K P L S D K L L R K V I Q V E L Q E A D G D C H L Q A F V L H L A Q R S I C I H P Q N P S L S Q W F E H Q E R K L H G T L P K L N F G M L R K M G
- Accession
- Protein length
Fragment
- Predicted molecular weight
10.2 kDa
- Actual molecular weight
10.2 kDa
- Amino acids
25 to 112
- Nature
Recombinant
Specifications
- Form
Lyophilized
- Additional notes
>= 95 % HPLC
General info
- Function
Chemotactic factor that attracts skin-associated memory T-lymphocytes. May play a role in mediating homing of lymphocytes to cutaneous sites. Binds to CCR10.
- Sequence similarities
Belongs to the intercrine beta (chemokine CC) family.
Storage
- Shipped at conditions
Ambient - Can Ship with Ice
- Appropriate short-term storage conditions
Ambient
- Appropriate long-term storage conditions
Ambient
This product is an active protein and may elicit a biological response in vivo, handle with caution.
Supplementary info
This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
CCL27 also known as CTACK (cutaneous T cell-attracting chemokine) is a chemokine that plays a critical role in skin immune surveillance. It has a mass of approximately 10 kDa. CCL27 is expressed in keratinocytes which are the predominant cells in the epidermis. It is also secreted by cells in inflamed skin. Researchers often use ELISA (enzyme-linked immunosorbent assay) techniques to study CCL27 due to its importance in skin immunology.
- Biological function summary
CCL27 attracts T cells to the skin's surface facilitating immune responses. Although not part of a larger complex CCL27 interacts with its receptor CCR10 on T cells directing them to skin sites where they can perform immune surveillance and respond to pathogens. This chemokine-receptor interaction is essential for maintaining skin homeostasis and effective immune responses.
- Pathways
The role of CCL27 gets significant attention in the CCR10-CTACK axis which is pivotal in the cutaneous immune system's functioning. Additionally the CCL27/CCR10 pathway has connections to the wider chemokine signaling network that regulates leukocyte trafficking. Through these associations CCL27 is connected to other chemokines and receptors including CCL28 which also shares CCR10 as a receptor highlighting the integrated nature of chemokine function in immune pathways.
- Associated diseases and disorders
CCL27 has been linked to various skin conditions such as psoriasis and atopic dermatitis. In psoriasis CCL27 recruits CCR10-expressing T cells to the skin aggravating the inflammatory response. Similarly in atopic dermatitis increased CCL27 levels may contribute to disease pathology by attracting immune cells that perpetuate inflammation. Understanding the interaction between CCL27 and these conditions may offer insight into potential therapeutic targets aimed at modulating chemokine signaling to alleviate symptoms.
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Biological Activity - Recombinant Human CCL27 protein (Active) (ab287948)
Fully biologically active determined by the dose dependent measurement of beta-arrestin recruitment on CCR10 using U2OS overexpressing human CCR10/beta-arrestin/beta-galactosidase complementation system. ED50 is ≤ 295 ng/ml, corresponding to a specific activity of 3.4 x 103 units/mg.
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