Recombinant Human CCL4/MIP-1 beta protein is a Human Full Length protein, in the 24 to 92 aa range, expressed in HEK 293, with >=95% purity, < 0.005 EU/µg endotoxin level and suitable for SDS-PAGE.
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Key facts
- Purity
- >=95% HPLC
- Endotoxin level
- < 0.005 EU/µg
- Expression system
- HEK 293 cells
- Tags
- Tag free
- Applications
- SDS-PAGE
- Biologically active
- No
Amino acid sequence
A P M G S D P P T A C C F S Y T A R K L P R N F V V D Y Y E T S S L C S Q P A V V F Q T K R S K Q V C A D P S E S W V Q E Y V Y D L E L N
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application SDS-PAGE | Reactivity Reacts | Dilution info - | Notes - |
Target data
Function
Monokine with inflammatory and chemokinetic properties. Binds to CCR5. One of the major HIV-suppressive factors produced by CD8+ T-cells. Recombinant MIP-1-beta induces a dose-dependent inhibition of different strains of HIV-1, HIV-2, and simian immunodeficiency virus (SIV). The processed form MIP-1-beta(3-69) retains the abilities to induce down-modulation of surface expression of the chemokine receptor CCR5 and to inhibit the CCR5-mediated entry of HIV-1 in T-cells. MIP-1-beta(3-69) is also a ligand for CCR1 and CCR2 isoform B.
Alternative names
LAG1, MIP1B, SCYA4, CCL4, C-C motif chemokine 4, G-26 T-lymphocyte-secreted protein, HC21, Lymphocyte activation gene 1 protein, MIP-1-beta(1-69), Macrophage inflammatory protein 1-beta, PAT 744, Protein H400, SIS-gamma, Small-inducible cytokine A4, T-cell activation protein 2, LAG-1, MIP-1-beta, ACT-2
Recommended products
Recombinant Human CCL4/MIP-1 beta protein is a Human Full Length protein, in the 24 to 92 aa range, expressed in HEK 293, with >=95% purity, < 0.005 EU/µg endotoxin level and suitable for SDS-PAGE.
Key facts
- Purity
- >=95% HPLC
- Endotoxin level
- < 0.005 EU/µg
- Expression system
- HEK 293 cells
- Tags
- Tag free
- Applications
- SDS-PAGE
- Biologically active
- No
- Accession
- P13236-1
- Animal free
- Yes
- Species
- Human
- Reconstitution
- Reconstitute in PBS
- Concentration
- Storage buffer
pH: 7.4
Constituents: 10.26% Trehalose, 0.727% Dibasic monohydrogen potassium phosphate, 0.248% Potassium phosphate monobasic
Sequence info
Amino acid sequence
- A P M G S D P P T A C C F S Y T A R K L P R N F V V D Y Y E T S S L C S Q P A V V F Q T K R S K Q V C A D P S E S W V Q E Y V Y D L E L N
- Accession
- P13236
- Protein length
- Full Length
- Predicted molecular weight
- 7.88 kDa
- Amino acids
- 24 to 92
- Nature
- Recombinant
Specifications
- Form
- Lyophilized
General info
- Function
Monokine with inflammatory and chemokinetic properties. Binds to CCR5. One of the major HIV-suppressive factors produced by CD8+ T-cells. Recombinant MIP-1-beta induces a dose-dependent inhibition of different strains of HIV-1, HIV-2, and simian immunodeficiency virus (SIV). The processed form MIP-1-beta(3-69) retains the abilities to induce down-modulation of surface expression of the chemokine receptor CCR5 and to inhibit the CCR5-mediated entry of HIV-1 in T-cells. MIP-1-beta(3-69) is also a ligand for CCR1 and CCR2 isoform B.
- Sequence similarities
Belongs to the intercrine beta (chemokine CC) family.
- Post-translational modifications
N-terminal processed form MIP-1-beta(3-69) is produced by proteolytic cleavage after secretion from peripheral blood lymphocytes.
Storage
- Shipped at conditions
- Ambient - Can Ship with Ice
- Appropriate short-term storage conditions
- Ambient
- Appropriate long-term storage conditions
- Ambient
- Storage information
- Avoid freeze / thaw cycle
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
CCL4 also known as MIP-1 beta or MIP-1b has a mechanical role as a chemokine involved in immune response regulation. The protein has a mass of about 7.8 kDa. It expresses in various tissues including leukocytes where it mainly functions as a chemoattractant. MIP-1 beta helps recruit immune cells like natural killer cells and monocytes to sites of inflammation or injury. Researchers frequently measure its levels using assays like MIP-1 beta ELISA or CCL4 ELISA.
- Biological function summary
CCL4 is critical in immune system modulation. It coordinates leukocyte activation and trafficking contributing to inflammation and immune surveillance. It does not function in isolation but rather interacts with other chemokines and receptors. CCL4 alongside its sibling chemokines forms a dynamic network that ensures a balanced immune response. It connects to receptors such as CCR5 playing substantial roles in its activities.
- Pathways
CCL4 participates in essential signaling cascades that mediate immune responses. It is notably part of the chemokine signaling pathway which is instrumental in directing cell movement. CCL4 also engages with proteins like CCR5 in the inflammatory and immune response pathways. This interaction enhances communication between cells required for effective pathogen defense.
- Associated diseases and disorders
CCL4 plays roles in immune-related conditions. It is strongly linked to inflammatory diseases such as rheumatoid arthritis and HIV infection. In rheumatoid arthritis CCL4 levels are often elevated correlating with inflammation severity. During HIV infection CCL4 interacts with proteins like CCR5 a co-receptor the virus uses to enter host cells. This involvement makes MIP-1 beta a target for studies seeking therapeutic interventions in these diseases.
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1 product image
SDS-PAGE - Recombinant Human CCL4/MIP-1 beta protein (ab283444)
SDS-PAGE analysis of ab283444.
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