Recombinant human CCNO protein (Active) is a Human Full Length protein, in the 1 to 350 aa range, expressed in Baculovirus infected Sf9, with >90% purity and suitable for SDS-PAGE, WB, FuncS.
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Key facts
- Purity
- >90% SDS-PAGE
- Expression system
- Baculovirus infected Sf9 cells
- Tags
- GST tag N-Terminus
- Applications
- SDS-PAGE, WB, FuncS
- Biologically active
- Yes
Sequence
This product is comprised of multiple sequences see
Amino acid sequence 1
M V T P C P T S P S S P A A R A G R R D N D Q N L R A P V K K S R R P R L R R K Q P L H P L N P C P L P G D S G I C D L F E S P S S G S D G A E S P S A A R G G S P L P G P A Q P V A Q L D L Q T F R D Y G Q S C Y A F R K A Q E S H F H P R E A L A R Q P Q V T A E S R C K L L S W L I P V H R Q F G L S F E S L C L T V N T L D R F L T T T P V A A D C F Q L L G V T S L L I A C K Q V E V H P P R V K Q L L A L C C G A F S R Q Q L C N L E C I V L H K L H F T L G A P T I S F F L E H F T H A R V E A G Q A E A S E A L E A Q A L A R G V A E L S L A D Y A F T S Y S P S L L A I C C L A L A D R M L R V S R P V D L R L G D H P E A A L E D C M G K L Q L L V A I N S T S L T H M L P V Q I C E K C S L P P S S K
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application SDS-PAGE | Reactivity Reacts | Dilution info - | Notes - |
Application WB | Reactivity Reacts | Dilution info - | Notes - |
Application FuncS | Reactivity Reacts | Dilution info - | Notes - |
Associated Products
Select an associated product type
1 product for Alternative Product
Target data
Function
Specifically required for generation of multiciliated cells, possibly by promoting a cell cycle state compatible with centriole amplification and maturation. Acts downstream of MCIDAS to promote mother centriole amplification and maturation in preparation for apical docking.
Alternative names
Cyclin-O, CCNO
Recommended products
Recombinant human CCNO protein (Active) is a Human Full Length protein, in the 1 to 350 aa range, expressed in Baculovirus infected Sf9, with >90% purity and suitable for SDS-PAGE, WB, FuncS.
Key facts
- Purity
- >90% SDS-PAGE
- Expression system
- Baculovirus infected Sf9 cells
- Tags
- GST tag N-Terminus
- Applications
- SDS-PAGE, WB, FuncS
- Biologically active
- Yes
- Biological activity
- The specific activity of Cdk2/ Cyclin O was determined to be 46 nmol/min/mg.
- Accession
- P22674-1
- Animal free
- No
- Species
- Human
- Concentration
- Storage buffer
pH: 7.5
Constituents: 25% Glycerol (glycerin, glycerine), 0.88% Sodium chloride, 0.79% Tris-HCl buffer, 0.31% Glutathione, 0.004% (R*,R*)-1,4-Dimercaptobutan-2,3-diol, 0.003% EDTA, 0.002% PMSF
Sequence info
Amino acid sequence 1
- M V T P C P T S P S S P A A R A G R R D N D Q N L R A P V K K S R R P R L R R K Q P L H P L N P C P L P G D S G I C D L F E S P S S G S D G A E S P S A A R G G S P L P G P A Q P V A Q L D L Q T F R D Y G Q S C Y A F R K A Q E S H F H P R E A L A R Q P Q V T A E S R C K L L S W L I P V H R Q F G L S F E S L C L T V N T L D R F L T T T P V A A D C F Q L L G V T S L L I A C K Q V E V H P P R V K Q L L A L C C G A F S R Q Q L C N L E C I V L H K L H F T L G A P T I S F F L E H F T H A R V E A G Q A E A S E A L E A Q A L A R G V A E L S L A D Y A F T S Y S P S L L A I C C L A L A D R M L R V S R P V D L R L G D H P E A A L E D C M G K L Q L L V A I N S T S L T H M L P V Q I C E K C S L P P S S K
- Accession
- P22674
- Protein length
- Full Length
- Predicted molecular weight
- 68 kDa
- Amino acids
- 1 to 350
- Nature
- Recombinant
- Tags
- GST tag N-Terminus
Amino acid sequence 2
- M E N F Q K V E K I G E G T Y G V V Y K A R N K L T G E V V A L K K I R L D T E T E G V P S T A I R E I S L L K E L N H P N I V K L L D V I H T E N K L Y L V F E F L H Q D L K K F M D A S A L T G I P L P L I K S Y L F Q L L Q G L A F C H S H R V L H R D L K P Q N L L I N T E G A I K L A D F G L A R A F G V P V R T Y T H E V V T L W Y R A P E I L L G C K Y Y S T A V D I W S L G C I F A E M V T R R A L F P G D S E I D Q L F R I F R T L G T P D E V V W P G V T S M P D Y K P S F P K W A R Q D F S K V V P P L D E D G R S L L S Q M L H Y D P N K R I S A K A A L A H P F F Q D V T K P V P H L R L
- Accession
- P24941
- Protein length
- Full Length
- Predicted molecular weight
- 58 kDa
- Amino acids
- 1 to 298
- Nature
- Recombinant
- Tags
- GST tag N-Terminus
Specifications
- Form
- Liquid
- Additional notes
Assessed by densitometry. Affinity purified.
General info
- Function
Specifically required for generation of multiciliated cells, possibly by promoting a cell cycle state compatible with centriole amplification and maturation. Acts downstream of MCIDAS to promote mother centriole amplification and maturation in preparation for apical docking.
- Sequence similarities
Belongs to the cyclin family.
- Subcellular localisation
- Nucleus, Nucleolus
Storage
- Shipped at conditions
- Dry Ice
- Appropriate short-term storage conditions
- -80°C
- Appropriate long-term storage conditions
- -80°C
- Storage information
- Avoid freeze / thaw cycle
This product is an active protein and may elicit a biological response in vivo, handle with caution.
Notes
ab89813 (Histone H1 protein) can be utilized as a substrate for assessing kinase activity
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
The CCNO gene encodes the Cyclin O protein which plays a role in cell cycle regulation particularly in the G2/M transition phase. Cyclin O a member of the cyclin protein family has a molecular mass of approximately 43 kDa. It is expressed in various tissues with higher expression observed in respiratory epithelial cells. Cyclin O interacts with cyclin-dependent kinases (CDKs) which are key regulators of cell cycle progression. This interaction is essential for maintaining proper cell division and genomic stability.
- Biological function summary
Cyclin O contributes to ciliogenesis the process by which cells build cilia. Cyclin O is part of a multiprotein complex that includes components necessary for the formation and function of motile cilia. Cilia play an important role in cellular processes such as signaling and movement of fluids across cell surfaces. Disruption in the cyclin O function can result in compromised ciliary function. This can impact various physiological processes like mucus clearance in respiratory pathways and fluid movement in brain ventricles.
- Pathways
Cyclin O is involved in the ciliogenesis pathway. It interacts with several other proteins like CDC28 and those involved in regulatory pathways of cell division ensuring that cilia are properly assembled and functional. Cyclin O's involvement in the proper assembly and maintenance of cilia illustrates its regulatory function within the Wnt signaling pathway which is a pathway critical for cell proliferation differentiation and polarity.
- Associated diseases and disorders
Cyclin O is associated with primary ciliary dyskinesia (PCD) a genetic disorder that affects the structure and function of cilia leading to chronic respiratory tract infections and problems in the reproductive system. Mutations in the CCNO gene have also been implicated in the development of certain ciliopathies disorders characterized by abnormalities in ciliary function. Cyclin O's relationship with proteins such as dynein is particularly noted in conditions where motility of cilia is affected linking it to the pathogenesis of these ciliopathy-related conditions.
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4 product images
Functional Studies - Recombinant human CCNO protein (Active) (ab177582)
The specific activity of CCNO (ab177582) was determined to be 43.8 nmol/min/mg as per activity assay protocol
SDS-PAGE - Recombinant human CCNO protein (Active) (ab177582)
SDS PAGE analysis of ab177582
Functional Studies - Recombinant human CCNO protein (Active) (ab177582)
The specific activity of Cdk2/ Cyclin O was determined to be 46 nmol/min/mg.
SDS-PAGE - Recombinant human CCNO protein (Active) (ab177582)
SDS-PAGE analysis of ab177852 which was determined to be >90% pure by densitometry. Cdk2 is approximately 58 kDa and Cyclin O is approximately 68 kDa.
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