Recombinant human CCR4 protein - Active (His tag) is a Human Full Length protein, in the 1 to 360 aa range, expressed in Mammalian, < 1 EU/µg endotoxin level and suitable for WB, FuncS.
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Key facts
- Endotoxin level
- < 1 EU/µg
- Expression system
- Mammalian
- Tags
- His tag C-Terminus
- Applications
- WB, FuncS
- Biologically active
- Yes
Amino acid sequence
M N P T D I A D T T L D E S I Y S N Y Y L Y E S I P K P C T K E G I K A F G E L F L P P L Y S L V F V F G L L G N S V V V L V L F K Y K R L R S M T D V Y L L N L A I S D L L F V F S L P F W G Y Y A A D Q W V F G L G L C K M I S W M Y L V G F Y S G I F F V M L M S I D R Y L A I V H A V F S L R A R T L T Y G V I T S L A T W S V A V F A S L P G F L F S T C Y T E R N H T Y C K T K Y S L N S T T W K V L S S L E I N I L G L V I P L G I M L F C Y S M I I R T L Q H C K N E K K N K A V K M I F A V V V L F L G F W T P Y N I V L F L E T L V E L E V L Q D C T F E R Y L D Y A I Q A T E T L A F V H C C L N P I I Y F F L G E K F R K Y I L Q L F K T C R G L F V L C Q Y C G L L Q I Y S A D T P S S S Y T Q S T M D H D L H D A L
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application WB | Reactivity Reacts | Dilution info - | Notes - |
Application FuncS | Reactivity Reacts | Dilution info - | Notes - |
Target data
Function
High affinity receptor for the C-C type chemokines CCL17/TARC, CCL22/MDC and CKLF isoform 1/CKLF1. The activity of this receptor is mediated by G(i) proteins which activate a phosphatidylinositol-calcium second messenger system. Can function as a chemoattractant homing receptor on circulating memory lymphocytes and as a coreceptor for some primary HIV-2 isolates. In the CNS, could mediate hippocampal-neuron survival.
Alternative names
CD194, CMKBR4, CCR4, C-C chemokine receptor type 4, C-C CKR-4, CC-CKR-4, CCR-4, K5-5
Recommended products
Recombinant human CCR4 protein - Active (His tag) is a Human Full Length protein, in the 1 to 360 aa range, expressed in Mammalian, < 1 EU/µg endotoxin level and suitable for WB, FuncS.
Key facts
- Endotoxin level
- < 1 EU/µg
- Expression system
- Mammalian
- Tags
- His tag C-Terminus
- Applications
- WB, FuncS
- Biologically active
- Yes
- Biological activity
- Measured by its binding ability in a functional ELISA. AB314529 at 10 μg/mL can bind an Anti-CCR4 recombinant antibody, the EC50 is 362.3-630.8 ng/mL.
- Accession
- P51679-1
- Animal free
- Yes
- Species
- Human
- Reconstitution
- Reconstitute in water
- Concentration
- Storage buffer
pH: 7.4 - 8
Constituents: 6% Trehalose, 0.14% PBS
Sequence info
Amino acid sequence
- M N P T D I A D T T L D E S I Y S N Y Y L Y E S I P K P C T K E G I K A F G E L F L P P L Y S L V F V F G L L G N S V V V L V L F K Y K R L R S M T D V Y L L N L A I S D L L F V F S L P F W G Y Y A A D Q W V F G L G L C K M I S W M Y L V G F Y S G I F F V M L M S I D R Y L A I V H A V F S L R A R T L T Y G V I T S L A T W S V A V F A S L P G F L F S T C Y T E R N H T Y C K T K Y S L N S T T W K V L S S L E I N I L G L V I P L G I M L F C Y S M I I R T L Q H C K N E K K N K A V K M I F A V V V L F L G F W T P Y N I V L F L E T L V E L E V L Q D C T F E R Y L D Y A I Q A T E T L A F V H C C L N P I I Y F F L G E K F R K Y I L Q L F K T C R G L F V L C Q Y C G L L Q I Y S A D T P S S S Y T Q S T M D H D L H D A L
- Accession
- P51679
- Protein length
- Full Length
- Predicted molecular weight
- 43.2 kDa
- Amino acids
- 1 to 360
- Nature
- Recombinant
- Tags
- His tag C-Terminus
Specifications
- Form
- Lyophilized
- Additional notes
Purification technique - Centrifugation.
General info
- Function
High affinity receptor for the C-C type chemokines CCL17/TARC, CCL22/MDC and CKLF isoform 1/CKLF1. The activity of this receptor is mediated by G(i) proteins which activate a phosphatidylinositol-calcium second messenger system. Can function as a chemoattractant homing receptor on circulating memory lymphocytes and as a coreceptor for some primary HIV-2 isolates. In the CNS, could mediate hippocampal-neuron survival.
- Sequence similarities
Belongs to the G-protein coupled receptor 1 family.
- Post-translational modifications
In natural killer cells, CCL22 binding induces phosphorylation on yet undefined Ser/Thr residues, most probably by beta-adrenergic receptor kinases 1 and 2.
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- -20°C, -80°C
- Appropriate long-term storage conditions
- -20°C, -80°C
- Storage information
- Avoid freeze / thaw cycle
This product is an active protein and may elicit a biological response in vivo, handle with caution.
Notes
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL. We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20degC/-80degC. Our default final concentration of glycerol is 50%.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
CCR4 also known as C-C chemokine receptor type 4 is a G protein-coupled receptor with the molecular mass of approximately 41 kDa. It is predominantly expressed on the surface of T-helper 2 cells regulatory T cells and certain dendritic cells. CCR4 functions by binding chemokines such as CCL17 and CCL22 facilitating chemotaxis of immune cells to sites of inflammation. Researchers often refer to antibodies targeting CCR4 like mogamulizumab when studying immune responses.
- Biological function summary
CCR4 plays an important role in the trafficking of immune cells to inflammatory sites therefore regulating immune response and tolerance. It is part of the chemokine receptor family which is integral to the immune system's function. CCR4 helps maintain the balance between effector and regulatory T cells contributing to immunological homeostasis. Its involvement in migration and positioning of immune cells within lymphoid and peripheral tissues highlights its importance in maintaining immune surveillance.
- Pathways
CCR4 significantly influences the chemokine signaling pathway which is pivotal in directing the movement and positioning of immune cells. This receptor interacts with key proteins like CCL17 and CCL22 to mediate cell chemotaxis in these pathways. Additionally CCR4 is associated with the T-cell receptor signaling pathway where it coordinates with other receptors and proteins to modulate immune responses.
- Associated diseases and disorders
Increased CCR4 expression has connections with various conditions such as adult T-cell leukemia/lymphoma and cutaneous T-cell lymphomas. In these diseases CCR4 assists tumor cells in evading the immune system fueling disease progression. Proteins like TARC (thymus and activation-regulated chemokine) and MDC (macrophage-derived chemokine) ligands for CCR4 often facilitate tumor cell migration and proliferation. Therapeutic approaches targeting CCR4 such as the anti-CCR4 antibody mogamulizumab have shown potential in treating these malignancies by disrupting these interactions.
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2 product images
Western blot - Recombinant human CCR4 protein - Active (His tag) (ab314529)
All lanes: mouse anti-6*His monoclonal antibody
All lanes: Western blot - Recombinant human CCR4 protein - Active (His tag) (ab314529)
Functional Studies - Recombinant human CCR4 protein - Active (His tag) (ab314529)
Measured by its binding ability in a functional ELISA. ab314529 at 10 μg/mL can bind an Anti-CCR4 recombinant antibody, the EC50 is 362.3-630.8 ng/mL.
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