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Recombinant human CD10 protein is a Human Fragment protein, in the 53 to 750 aa range, expressed in Baculovirus infected Sf9, with >95% purity and suitable for SDS-PAGE, FuncS.
Alternative names=Neprilysin, Atriopeptidase, Common acute lymphocytic leukemia antigen, Enkephalinase, Neutral endopeptidase 24.11, Skin fibroblast elastase, CALLA, NEP, Neutral endopeptidase, SFE, EPN, MME
>95% SDS-PAGE
Baculovirus infected Sf9 cells
His tag N-Terminus
SDS-PAGE, FuncS
Yes
D D G I C K S S D C I K S A A R L I Q N M D A T T E P C T D F F K Y A C G G W L K R N V I P E T S S R Y G N F D I L R D E L E V V L K D V L Q E P K T E D I V A V Q K A K A L Y R S C I N E S A I D S R G G E P L L K L L P D I Y G W P V A T E N W E Q K Y G A S W T A E K A I A Q L N S K Y G K K V L I N L F V G T D D K N S V N H V I H I D Q P R L G L P S R D Y Y E C T G I Y K E A C T A Y V D F M I S V A R L I R Q E E R L P I D E N Q L A L E M N K V M E L E K E I A N A T A K P E D R N D P M L L Y N K M T L A Q I Q N N F S L E I N G K P F S W L N F T N E I M S T V N I S I T N E E D V V V Y A P E Y L T K L K P I L T K Y S A R D L Q N L M S W R F I M D L V S S L S R T Y K E S R N A F R K A L Y G T T S E T A T W R R C A N Y V N G N M E N A V G R L Y V E A A F A G E S K H V V E D L I A Q I R E V F I Q T L D D L T W M D A E T K K R A E E K A L A I K E R I G Y P D D I V S N D N K L N N E Y L E L N Y K E D E Y F E N I I Q N L K F S Q S K Q L K K L R E K V D K D E W I S G A A V V N A F Y S S G R N Q I V F P A G I L Q P P F F S A Q Q S N S L N Y G G I G M V I G H E I T H G F D D N G R N F N K D G D L V D W W T Q Q S A S N F K E Q S Q C M V Y Q Y G N F S W D L A G G Q H L N G I N T L G E N I A D N G G L G Q A Y R A Y Q N Y I K K N G E E K L L P G L D L N H K Q L F F L N F A Q V W C G T Y R P E Y A V N S I K T D V H S P G N F R I I G T L Q N S A E F S E A F H C R K N S Y M N P E K K C R V W
Application | Reactivity | Dilution info | Notes |
---|---|---|---|
Application SDS-PAGE | Reactivity Reacts | Dilution info - | Notes - |
Application FuncS | Reactivity Reacts | Dilution info - | Notes - |
Recombinant human CD10 protein is a Human Fragment protein, in the 53 to 750 aa range, expressed in Baculovirus infected Sf9, with >95% purity and suitable for SDS-PAGE, FuncS.
Alternative names=Neprilysin, Atriopeptidase, Common acute lymphocytic leukemia antigen, Enkephalinase, Neutral endopeptidase 24.11, Skin fibroblast elastase, CALLA, NEP, Neutral endopeptidase, SFE, EPN, MME
>95% SDS-PAGE
Baculovirus infected Sf9 cells
His tag N-Terminus
SDS-PAGE, FuncS
Yes
1140 pmol/min/μg (9 ng NEP were used to cleave 10μM Mca-RPPGFSAFK(Dnp)-OH (Fluorogenic Substrate) at 37°C in 50 mM TRIS, pH 9.0).
85 kDa
53 to 750
D D G I C K S S D C I K S A A R L I Q N M D A T T E P C T D F F K Y A C G G W L K R N V I P E T S S R Y G N F D I L R D E L E V V L K D V L Q E P K T E D I V A V Q K A K A L Y R S C I N E S A I D S R G G E P L L K L L P D I Y G W P V A T E N W E Q K Y G A S W T A E K A I A Q L N S K Y G K K V L I N L F V G T D D K N S V N H V I H I D Q P R L G L P S R D Y Y E C T G I Y K E A C T A Y V D F M I S V A R L I R Q E E R L P I D E N Q L A L E M N K V M E L E K E I A N A T A K P E D R N D P M L L Y N K M T L A Q I Q N N F S L E I N G K P F S W L N F T N E I M S T V N I S I T N E E D V V V Y A P E Y L T K L K P I L T K Y S A R D L Q N L M S W R F I M D L V S S L S R T Y K E S R N A F R K A L Y G T T S E T A T W R R C A N Y V N G N M E N A V G R L Y V E A A F A G E S K H V V E D L I A Q I R E V F I Q T L D D L T W M D A E T K K R A E E K A L A I K E R I G Y P D D I V S N D N K L N N E Y L E L N Y K E D E Y F E N I I Q N L K F S Q S K Q L K K L R E K V D K D E W I S G A A V V N A F Y S S G R N Q I V F P A G I L Q P P F F S A Q Q S N S L N Y G G I G M V I G H E I T H G F D D N G R N F N K D G D L V D W W T Q Q S A S N F K E Q S Q C M V Y Q Y G N F S W D L A G G Q H L N G I N T L G E N I A D N G G L G Q A Y R A Y Q N Y I K K N G E E K L L P G L D L N H K Q L F F L N F A Q V W C G T Y R P E Y A V N S I K T D V H S P G N F R I I G T L Q N S A E F S E A F H C R K N S Y M N P E K K C R V W
Fragment
No
Recombinant
Human
Constituents: 20% Glycerol (glycerin, glycerine), 1.75% Sodium chloride, 0.61% Tris
Liquid
ab157051 has been purified in the glycosylated soluble form.
Thermolysin-like specificity, but is almost confined on acting on polypeptides of up to 30 amino acids (PubMed:15283675, PubMed:8168535). Biologically important in the destruction of opioid peptides such as Met- and Leu-enkephalins by cleavage of a Gly-Phe bond (PubMed:17101991). Able to cleave angiotensin-1, angiotensin-2 and angiotensin 1-9 (PubMed:15283675). Involved in the degradation of atrial natriuretic factor (ANF) and brain natriuretic factor (BNP(1-32)) (PubMed:2531377, PubMed:2972276, PubMed:16254193). Displays UV-inducible elastase activity toward skin preelastic and elastic fibers (PubMed:20876573).
Belongs to the peptidase M13 family.
Myristoylation is a determinant of membrane targeting.
Blue Ice
-80°C
Upon delivery aliquot
Avoid freeze / thaw cycle
This product is an active protein and may elicit a biological response in vivo, handle with caution.
Stable on ice for at least 1h. Do not freeze in dilute form.
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