Recombinant human CD10 protein is a Human Fragment protein, in the 53 to 750 aa range, expressed in Baculovirus infected Sf9, with >95% purity and suitable for SDS-PAGE, FuncS.
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- Advanced science (Weinheim, Baden-Wurttemberg, Germany) 8:e21018482021A CD10-OGP Membrane Peptolytic Signaling Axis in Fibroblasts Regulates Lipid Metabolism of Cancer Stem Cells via SCD1.Applications:Unspecified applicationReactive species:Unspecified reactive speciesShubin Yu,Yiwen Lu,An Su,Jianing Chen,Jiang Li,Boxuan Zhou,Xinwei Liu,Qidong Xia,Yihong Li,Jiaqian Li,Min Huang,Yingying Ye,Qiyi Zhao,Sushi Jiang,Xiaoqing Yan,Xiaojuan Wang,Can Di,Jiayao Pan,Shicheng SuPubMed 34363355
Key facts
- Purity
- >95% SDS-PAGE
- Expression system
- Baculovirus infected Sf9 cells
- Tags
- His tag N-Terminus
- Applications
- SDS-PAGE, FuncS
- Biologically active
- Yes
Amino acid sequence
D D G I C K S S D C I K S A A R L I Q N M D A T T E P C T D F F K Y A C G G W L K R N V I P E T S S R Y G N F D I L R D E L E V V L K D V L Q E P K T E D I V A V Q K A K A L Y R S C I N E S A I D S R G G E P L L K L L P D I Y G W P V A T E N W E Q K Y G A S W T A E K A I A Q L N S K Y G K K V L I N L F V G T D D K N S V N H V I H I D Q P R L G L P S R D Y Y E C T G I Y K E A C T A Y V D F M I S V A R L I R Q E E R L P I D E N Q L A L E M N K V M E L E K E I A N A T A K P E D R N D P M L L Y N K M T L A Q I Q N N F S L E I N G K P F S W L N F T N E I M S T V N I S I T N E E D V V V Y A P E Y L T K L K P I L T K Y S A R D L Q N L M S W R F I M D L V S S L S R T Y K E S R N A F R K A L Y G T T S E T A T W R R C A N Y V N G N M E N A V G R L Y V E A A F A G E S K H V V E D L I A Q I R E V F I Q T L D D L T W M D A E T K K R A E E K A L A I K E R I G Y P D D I V S N D N K L N N E Y L E L N Y K E D E Y F E N I I Q N L K F S Q S K Q L K K L R E K V D K D E W I S G A A V V N A F Y S S G R N Q I V F P A G I L Q P P F F S A Q Q S N S L N Y G G I G M V I G H E I T H G F D D N G R N F N K D G D L V D W W T Q Q S A S N F K E Q S Q C M V Y Q Y G N F S W D L A G G Q H L N G I N T L G E N I A D N G G L G Q A Y R A Y Q N Y I K K N G E E K L L P G L D L N H K Q L F F L N F A Q V W C G T Y R P E Y A V N S I K T D V H S P G N F R I I G T L Q N S A E F S E A F H C R K N S Y M N P E K K C R V W
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application SDS-PAGE | Reactivity Reacts | Dilution info - | Notes - |
Application FuncS | Reactivity Reacts | Dilution info - | Notes - |
Associated Products
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2 products for Alternative Product
Target data
Function
Thermolysin-like specificity, but is almost confined on acting on polypeptides of up to 30 amino acids (PubMed:15283675, PubMed:6208535, PubMed:6349683, PubMed:8168535). Biologically important in the destruction of opioid peptides such as Met- and Leu-enkephalins by cleavage of a Gly-Phe bond (PubMed:17101991, PubMed:6349683). Catalyzes cleavage of bradykinin, substance P and neurotensin peptides (PubMed:6208535). Able to cleave angiotensin-1, angiotensin-2 and angiotensin 1-9 (PubMed:15283675, PubMed:6349683). Involved in the degradation of atrial natriuretic factor (ANF) and brain natriuretic factor (BNP(1-32)) (PubMed:16254193, PubMed:2531377, PubMed:2972276). Displays UV-inducible elastase activity toward skin preelastic and elastic fibers (PubMed:20876573).
Alternative names
CD10, EPN, MME, Neprilysin, Atriopeptidase, Common acute lymphocytic leukemia antigen, Enkephalinase, Neutral endopeptidase 24.11, Skin fibroblast elastase, CALLA, NEP, Neutral endopeptidase, SFE
Recommended products
Recombinant human CD10 protein is a Human Fragment protein, in the 53 to 750 aa range, expressed in Baculovirus infected Sf9, with >95% purity and suitable for SDS-PAGE, FuncS.
Key facts
- Purity
- >95% SDS-PAGE
- Expression system
- Baculovirus infected Sf9 cells
- Tags
- His tag N-Terminus
- Applications
- SDS-PAGE, FuncS
- Biologically active
- Yes
- Biological activity
- 1140 pmol/min/μg (9 ng NEP were used to cleave 10μM Mca-RPPGFSAFK(Dnp)-OH (Fluorogenic Substrate) at 37°C in 50 mM TRIS, pH 9.0).
- Accession
- P08473-1
- Animal free
- No
- Species
- Human
- Concentration
- Storage buffer
Constituents: 20% Glycerol (glycerin, glycerine), 1.75% Sodium chloride, 0.61% Tris
Sequence info
Amino acid sequence
- D D G I C K S S D C I K S A A R L I Q N M D A T T E P C T D F F K Y A C G G W L K R N V I P E T S S R Y G N F D I L R D E L E V V L K D V L Q E P K T E D I V A V Q K A K A L Y R S C I N E S A I D S R G G E P L L K L L P D I Y G W P V A T E N W E Q K Y G A S W T A E K A I A Q L N S K Y G K K V L I N L F V G T D D K N S V N H V I H I D Q P R L G L P S R D Y Y E C T G I Y K E A C T A Y V D F M I S V A R L I R Q E E R L P I D E N Q L A L E M N K V M E L E K E I A N A T A K P E D R N D P M L L Y N K M T L A Q I Q N N F S L E I N G K P F S W L N F T N E I M S T V N I S I T N E E D V V V Y A P E Y L T K L K P I L T K Y S A R D L Q N L M S W R F I M D L V S S L S R T Y K E S R N A F R K A L Y G T T S E T A T W R R C A N Y V N G N M E N A V G R L Y V E A A F A G E S K H V V E D L I A Q I R E V F I Q T L D D L T W M D A E T K K R A E E K A L A I K E R I G Y P D D I V S N D N K L N N E Y L E L N Y K E D E Y F E N I I Q N L K F S Q S K Q L K K L R E K V D K D E W I S G A A V V N A F Y S S G R N Q I V F P A G I L Q P P F F S A Q Q S N S L N Y G G I G M V I G H E I T H G F D D N G R N F N K D G D L V D W W T Q Q S A S N F K E Q S Q C M V Y Q Y G N F S W D L A G G Q H L N G I N T L G E N I A D N G G L G Q A Y R A Y Q N Y I K K N G E E K L L P G L D L N H K Q L F F L N F A Q V W C G T Y R P E Y A V N S I K T D V H S P G N F R I I G T L Q N S A E F S E A F H C R K N S Y M N P E K K C R V W
- Accession
- P08473
- Protein length
- Fragment
- Predicted molecular weight
- 85 kDa
- Amino acids
- 53 to 750
- Nature
- Recombinant
- Tags
- His tag N-Terminus
Specifications
- Form
- Liquid
- Additional notes
ab157051 has been purified in the glycosylated soluble form.
General info
- Function
Thermolysin-like specificity, but is almost confined on acting on polypeptides of up to 30 amino acids (PubMed:15283675, PubMed:6208535, PubMed:6349683, PubMed:8168535). Biologically important in the destruction of opioid peptides such as Met- and Leu-enkephalins by cleavage of a Gly-Phe bond (PubMed:17101991, PubMed:6349683). Catalyzes cleavage of bradykinin, substance P and neurotensin peptides (PubMed:6208535). Able to cleave angiotensin-1, angiotensin-2 and angiotensin 1-9 (PubMed:15283675, PubMed:6349683). Involved in the degradation of atrial natriuretic factor (ANF) and brain natriuretic factor (BNP(1-32)) (PubMed:16254193, PubMed:2531377, PubMed:2972276). Displays UV-inducible elastase activity toward skin preelastic and elastic fibers (PubMed:20876573).
- Sequence similarities
Belongs to the peptidase M13 family.
- Post-translational modifications
Myristoylation is a determinant of membrane targeting.
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- -80°C
- Appropriate long-term storage conditions
- -80°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
This product is an active protein and may elicit a biological response in vivo, handle with caution.
Notes
Stable on ice for at least 1h. Do not freeze in dilute form.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
CD10 also known as neprilysin or neutral endopeptidase is a zinc-dependent metalloprotease enzyme with a mass of approximately 100 kDa. This enzyme is expressed across a variety of tissues including kidney lung and neutrophils. Its expression is also notable in the lymphoid tissues. CD10 functions by cleaving peptides at the N-terminal of hydrophobic residues. Common names used in literature for CD10 include CALLA (common acute lymphoblastic leukemia antigen) and membrane metalloendopeptidase.
- Biological function summary
CD10 regulates peptide signaling by inactivating various bioactive peptides such as enkephalins atrial natriuretic peptide and bradykinin. It does not function as part of a complex but rather as a standalone entity. The ability of CD10 to hydrolyze peptides influences signal transmission across cellular membranes impacting cellular communication and signal termination within different systems.
- Pathways
Neprilysin plays an essential role in hydrolyzing signaling peptides in several critical pathways. It is especially significant in the natriuretic peptide system and the renin-angiotensin system maintaining blood pressure and fluid balance. Within these pathways CD10 modifies peptides working alongside proteins like atrial natriuretic peptide synthase and angiotensin-converting enzyme ensuring systemic homeostasis and cardiovascular health.
- Associated diseases and disorders
CD10 shows a significant connection to certain types of cancer and Alzheimer's disease. In oncology altered expression of CD10 serves as a CD10 marker in cancers such as lymphomas and some leukemia forms which helps in diagnosis and treatment decisions. Additionally CD10's involvement in neuropeptide degradation links it to Amyloid beta peptide accumulation in Alzheimer's where its dysregulation may promote disease progression. Related proteins are amyloid precursor protein in Alzheimer's and CD19 often co-expressed with CD10 in some lymphomas highlighting its role in both diagnostic and therapeutic contexts.
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SDS-PAGE - Recombinant human CD10 protein (ab157051)
SDS-PAGE Analysis of 1.0 μg of purified ab157051.
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