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Recombinant human CD105 protein is a Human Fragment protein, in the 26 to 584 aa range, expressed in Insect, with >95% purity and suitable for ELISA, WB, SDS-PAGE.

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Purity

>95% SDS-PAGE

Expression system

Insect cells

Tags

His tag C-Terminus

Applications

ELISA, WB, SDS-PAGE

Biologically active

Yes

Sequence

E T V H C D L Q P V G P E R G E V T Y T T S Q V S K G C V A Q A P N A I L E V H V L F L E F P T G P S Q L E L T L Q A S K Q N G T W P R E V L L V L S V N S S V F L H L Q A L G I P L H L A Y N S S L V T F Q E P P G V N T T E L P S F P K T Q I L E W A A E R G P I T S A A E L N D P Q S I L L R L G Q A Q G S L S F C M L E A S Q D M G R T L E W R P R T P A L V R G C H L E G V A G H K E A H I L R V L P G H S A G P R T V T V K V E L S C A P G D L D A V L I L Q G P P Y V S W L I D A N H N M Q I W T T G E Y S F K I F P E K N I R G F K L P D T P Q G L L G E A R M L N A S I V A S F V E L P L A S I V S L H A S S C G G R L Q T S P A P I Q T T P P K D T C S P E L L M S L I Q T K C A D D A M T L V L K K E L V A H L K C T I T G L T F W D P S C E A E D R G D K F V L R S A Y S S C G M Q V S A S M I S N E A V V N I L S S S S P Q R K K V H C L N M D S L S F Q L G L Y L S P H F L Q A S N T I E P G Q Q S F V Q V R V S P S V S E F L L Q L D S C H L D L G P E G G T V E L I Q G R A A K G N C V S L L S P S P E G D P R F S F L L H F Y T V P I P K T G T L S C T V A L R P K T G S Q D Q E V H R T V F M R L N I I S P D L S G C T S H H H H H H

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Shipping To:
United States

Reactivity data

Application
ELISA
Reactivity
Reacts
Dilution info
-
Notes

-

Application
WB
Reactivity
Reacts
Dilution info
-
Notes
Ab54338 can be used as a WB positive control in conjunction with ab85956.
Application
SDS-PAGE
Reactivity
Reacts
Dilution info
-
Notes
This product has a calculated monomeric molecular mass of 61 kDa but as a result of glycosylation, migrates at approximately 75 - 85 kDa under reducing conditions in SDS-PAGE.

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Target data

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Recombinant human CD105 protein is a Human Fragment protein, in the 26 to 584 aa range, expressed in Insect, with >95% purity and suitable for ELISA, WB, SDS-PAGE.

Key facts

Purity

>95% SDS-PAGE

Expression system

Insect cells

Applications

ELISA, WB, SDS-PAGE

Biological activity

Active

Amino acids

26 to 584

Sequence

E T V H C D L Q P V G P E R G E V T Y T T S Q V S K G C V A Q A P N A I L E V H V L F L E F P T G P S Q L E L T L Q A S K Q N G T W P R E V L L V L S V N S S V F L H L Q A L G I P L H L A Y N S S L V T F Q E P P G V N T T E L P S F P K T Q I L E W A A E R G P I T S A A E L N D P Q S I L L R L G Q A Q G S L S F C M L E A S Q D M G R T L E W R P R T P A L V R G C H L E G V A G H K E A H I L R V L P G H S A G P R T V T V K V E L S C A P G D L D A V L I L Q G P P Y V S W L I D A N H N M Q I W T T G E Y S F K I F P E K N I R G F K L P D T P Q G L L G E A R M L N A S I V A S F V E L P L A S I V S L H A S S C G G R L Q T S P A P I Q T T P P K D T C S P E L L M S L I Q T K C A D D A M T L V L K K E L V A H L K C T I T G L T F W D P S C E A E D R G D K F V L R S A Y S S C G M Q V S A S M I S N E A V V N I L S S S S P Q R K K V H C L N M D S L S F Q L G L Y L S P H F L Q A S N T I E P G Q Q S F V Q V R V S P S V S E F L L Q L D S C H L D L G P E G G T V E L I Q G R A A K G N C V S L L S P S P E G D P R F S F L L H F Y T V P I P K T G T L S C T V A L R P K T G S Q D Q E V H R T V F M R L N I I S P D L S G C T S H H H H H H

Accession
P17813-1
Protein length

Fragment

Animal free

No

Nature

Recombinant

Species

Human

Reconstitution

Reconstitute in PBS or medium to a concentration not lower than 50 µg/ml.

Concentration
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Specifications

Form

Lyophilized

Additional notes

Purity: > 95% (SDS-PAGE and visualized by Silverstain). Affinity purified.

General info

Function

Vascular endothelium glycoprotein that plays an important role in the regulation of angiogenesis (PubMed:21737454, PubMed:23300529). Required for normal structure and integrity of adult vasculature (PubMed:7894484). Regulates the migration of vascular endothelial cells (PubMed:17540773). Required for normal extraembryonic angiogenesis and for embryonic heart development (By similarity). May regulate endothelial cell shape changes in response to blood flow, which drive vascular remodeling and establishment of normal vascular morphology during angiogenesis (By similarity). May play a critical role in the binding of endothelial cells to integrins and/or other RGD receptors (PubMed:1692830). Acts as TGF-beta coreceptor and is involved in the TGF-beta/BMP signaling cascade that ultimately leads to the activation of SMAD transcription factors (PubMed:8370410, PubMed:21737454, PubMed:22347366, PubMed:23300529). Required for GDF2/BMP9 signaling through SMAD1 in endothelial cells and modulates TGFB1 signaling through SMAD3 (PubMed:21737454, PubMed:22347366, PubMed:23300529).

Storage

Shipped at conditions

Blue Ice

Appropriate long-term storage conditions

-20°C

Storage information

Avoid freeze / thaw cycle

This product is an active protein and may elicit a biological response in vivo, handle with caution.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

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Terms & Conditions.

Downloads

Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

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