JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB171473

Recombinant Human CD116 protein (denatured) (His tag N-Terminus)

Be the first to review this product! Submit a review

|

(0 Publication)

Recombinant Human CD116 protein (denatured) (His tag N-Terminus) is a Human Fragment protein, in the 20 to 320 aa range, expressed in Escherichia coli, with >85%, suitable for SDS-PAGE.

View Alternative Names

CD116, CSF2R, CSF2RY, CSF2RA, Granulocyte-macrophage colony-stimulating factor receptor subunit alpha, GM-CSF-R-alpha, GMCSFR-alpha, GMR-alpha, CDw116

1 Images
SDS-PAGE - Recombinant Human CD116 protein (denatured) (His tag N-Terminus) (AB171473)
  • SDS-PAGE

Unknown

SDS-PAGE - Recombinant Human CD116 protein (denatured) (His tag N-Terminus) (AB171473)

15% SDS-PAGE analysis of 3µg ab171473.

Key facts

Purity

>85% SDS-PAGE

Expression system

Escherichia coli

Tags

His tag N-Terminus

Applications

SDS-PAGE

applications

Biologically active

No

Accession

P15509

Animal free

No

Carrier free

No

Species

Human

Storage buffer

pH: 8 Constituents: 10% Glycerol (glycerin, glycerine), 2.4% Urea, 0.32% Tris HCl

storage-buffer

style
left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
style
left-column

Product details

This product was previously labelled as GM-CSF Receptor alpha
style
left-column

Sequence info

[{"sequence":"MGSSHHHHHHSSGLVPRGSHMGSLIPEKSDLRTVAPASSLNVRFDSRTMNLSWDCQENTTFSKCFLTDKKNRVVEPRLSNNECSCTFREICLHEGVTFEVHVNTSQRGFQQKLLYPNSGREGTAAQNFSCFIYNADLMNCTWARGPTAPRDVQYFLYIRNSKRRREIRCPYYIQDSGTHVGCHLDNLSGLTSRNYFLVNGTSREIGIQFFDSLLDTKKIERFNPPSNVTVRCNTTHCLVRWKQPRTYQKLSYLDFQYQLDVHRKNTQPGTENLLINVSGDLENRYNFPSSEPRAKHSVKIRAADVRILNWSSWSEAIEFGSDDG","proteinLength":"Fragment","predictedMolecularWeight":"37.2 kDa","actualMolecularWeight":null,"aminoAcidEnd":320,"aminoAcidStart":20,"nature":"Recombinant","expressionSystem":"Escherichia coli","accessionNumber":"P15509","tags":[{"tag":"His","terminus":"N-Terminus"}]}]
style
left-column

Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
False
style
left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

CD116 also known as the CSF2RA (colony-stimulating factor 2 receptor alpha subunit) is a protein that plays an important role in cytokine receptor signaling. It has a mass of approximately 43 kDa. This receptor is widely expressed on the surface of hematopoietic cells including monocytes granulocytes and some T-lymphocytes. Furthermore CD116 presents important interactions with cellular functioning by being part of a heterodimeric receptor complex. It couples with the beta subunit (CSF2RB) together mediating effects of granulocyte-macrophage colony-stimulating factor (GM-CSF).
Biological function summary

The CSF2RA is integral in facilitating cellular communication. It belongs to the cytokine receptor family specifically binding the GM-CSF. This molecule triggers signaling cascades that are essential for the proliferation differentiation and survival of various blood cells. When GM-CSF binds to the receptor complex made of CD116 and CSF2RB it activates downstream pathways influencing these critical processes thereby ensuring proper bone marrow function and immune cell activity.

Pathways

CD116 participates in significant signal transduction cascades. It is involved in the JAK-STAT pathway which plays an important role in regulating immune responses and hematopoiesis. The engagement of GM-CSF with CD116 triggers the activation of JAK kinases followed by STAT protein phosphorylation. Another pathway linked with CD116 is the MAPK pathway which further mediates downstream signals that modulate cellular growth and differentiation integrating input from various activating signals.

Abnormalities in CD116 expression and function associate with several health conditions. One example is chronic myelomonocytic leukemia (CMML) where deregulated signaling through the GM-CSF receptor impacts cell proliferation and differentiation. Aberrant CD116 activity also ties into inflammation and autoimmune disorders such as rheumatoid arthritis where overactive signaling can worsen inflammatory responses. Proteins like GM-CSF and its beta receptor subunit CSF2RB accompany these disorders by contributing to the pathway dysregulation observed in these disease states.
style
left-column

Specifications

Form

Liquid

style
left-column

General info

Function

Low affinity receptor for granulocyte-macrophage colony-stimulating factor. Transduces a signal that results in the proliferation, differentiation, and functional activation of hematopoietic cells.

Sequence similarities

Belongs to the type I cytokine receptor family. Type 5 subfamily.

style
left-column

Product protocols

style
left-column

Target data

Low affinity receptor for granulocyte-macrophage colony-stimulating factor. Transduces a signal that results in the proliferation, differentiation, and functional activation of hematopoietic cells.
See full target information CSF2RA
style
left-column
style
left-column
style
right-column

Complementary Products

Primary Antibodies

AB308295

Anti-CD116 antibody [EPR26505-444] - BSA and Azide free

RabMAb|Recombinant

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD116 antibody [EPR26505-444] - BSA and Azide free (AB308295)

  • 0
  • 0
Secondary Antibodies

AB150113

Goat Anti-Mouse IgG H&L (Alexa Fluor® 488)

Immunocytochemistry/ Immunofluorescence - Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) (AB150113)

  • 5
  • 15
Proteins & Peptides

AB87361

Recombinant Human 14-3-3 zeta protein (His tag N-Terminus)

SDS-PAGE - Recombinant Human 14-3-3 zeta protein (AB87361)

  • 0
  • 0
Proteins & Peptides

AB140412

Recombinant Human STAT1 alpha protein - GST tagged

SDS-PAGE - Recombinant Human STAT1 alpha protein - GST tagged (AB140412)

  • 0
  • 0

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com