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AB160218

Recombinant Human CD133 protein

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(2 Publications)

Recombinant Human CD133 protein is a Human Full Length protein, in the 1 to 856 aa range, expressed in Wheat germ, suitable for SDS-PAGE, ELISA, WB.

View Alternative Names

CD133, PROML1, MSTP061, PROM1, Prominin-1, Antigen AC133, Prominin-like protein 1

1 Images
SDS-PAGE - Recombinant Human CD133 protein (AB160218)
  • SDS-PAGE

Supplier Data

SDS-PAGE - Recombinant Human CD133 protein (AB160218)

12.5% SDS-PAGE analysis of ab160218 stained with Coomassie Blue.

Key facts

Expression system

Wheat germ

Tags

GST tag N-Terminus

Applications

WB, ELISA, SDS-PAGE

applications

Biologically active

No

Accession

O43490

Animal free

No

Carrier free

No

Species

Human

Storage buffer

Constituents: 0.79% Tris HCl, 0.31% Glutathione

storage-buffer

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "ELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "WB": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Sequence info

[{"sequence":"MALVLGSLLLLGLCGNSFSGGQPSSTDAPKAWNYELPATNYETQDSHKAGPIGILFELVHIFLYVVQPRDFPEDTLRKFLQKAYESKIDYDKIVYYEAGIILCCVLGLLFIILMPLVGYFFCMCRCCNKCGGEMHQRQKENGPFLRKCFAISLLVICIIISIGIFYGFVANHQVRTRIKRSRKLADSNFKDLRTLLNETPEQIKYILAQYNTTKDKAFTDLNSINSVLGGGILDRLRPNIIPVLDEIKSMATAIKETKEALENMNSTLKSLHQQSTQLSSSLTSVKTSLRSSLNDPLCLVHPSSETCNSIRLSLSQLNSNPELRQLPPVDAELDNVNNVLRTDLDGLVQQGYQSLNDIPDRVQRQTTTVVAGIKRVLNSIGSDIDNVTQRLPIQDILSAFSVYVNNTESYIHRNLPTLEEYDSYWWLGGLVICSLLTLIVIFYYLGLLCGVCGYDRHATPTTRGCVSNTGGVFLMVGVGLSFLFCWILMIIVVLTFVFGANVEKLICEPYTSKELFRVLDTPYLLNEDWEYYLSGKLFNKSKMKLTFEQVYSDCKKNRGTYGTLHLQNSFNISEHLNINEHTGSISSELESLKVNLNIFLLGAAGRKNLQDFAACGIDRMNYDSYLAQTGKSPAGVNLLSFAYDLEAKANSLPPGNLRNSLKRDAQTIKTIHQQRVLPIEQSLSTLYQSVKILQRTGNGLLERVTRILASLDFAQNFITNNTSSVIIEETKKYGRTIIGYFEHYLQWIEFSISEKVASCKPVATALDTAVDVFLCSYIIDPLNLFWFGIGKATVFLLPALIFAVKLAKYYRRMDSEDVYDDVETIPMKNMENGNNGYHKDHVYGIHNPVMTSPSQH","proteinLength":"Full Length","predictedMolecularWeight":"122.7 kDa","actualMolecularWeight":null,"aminoAcidEnd":856,"aminoAcidStart":1,"nature":"Recombinant","expressionSystem":"Wheat germ","accessionNumber":"O43490","tags":[{"tag":"GST","terminus":"N-Terminus"}]}]
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Properties and storage information

Shipped at conditions
Dry Ice
Appropriate short-term storage conditions
-80°C
Appropriate long-term storage conditions
-80°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
False
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

CD133 also known as Prominin-1 is a transmembrane glycoprotein with a molecular weight of approximately 120 kDa. This protein stands out due to its expression in various stem and progenitor cells including those of the neural hematopoietic and epithelial lineages. CD133 is recognized for its distinct five transmembrane domains and two large extracellular loops. Researchers commonly use CD133 as a cell surface marker to identify stem and progenitor cells. The significance of CD133 extends to immunohistochemistry (IHC) and Western blot applications where it is a popular target for detection and analysis.
Biological function summary

CD133 is involved in maintaining the cellular architecture and signaling processes. It plays a role in cellular differentiation and proliferation while acting as a part of larger protein complexes on the cell surface. This protein is notable as it contributes significantly to the regulation of stem cell properties supporting the growth and repair in various tissues. Anti-CD133 antibodies are valuable tools in studying these functions due to their specificity in targeting this marker.

Pathways

CD133 participates in cellular signaling and transport pathways vital for intercellular communication and homeostasis. It interacts within pathways related to cell polarity and asymmetric division both essential for tissue development and maintenance. CD133 is associated with proteins like CD133/1 and CD133/2 which further highlights its involvement in these complex biological networks and on pathways such as the Wnt signaling pathway which regulates gene expression associated with cell proliferation and differentiation.

Several studies indicate a strong relationship between CD133 expression and certain cancers such as glioblastoma and colorectal cancer. In these cases CD133 serves not only as a marker but also as an indicator of the presence of cancer stem cells which are capable of tumor initiation and maintenance. Furthermore researchers have identified connections between CD133 and other proteins such as c-Met (cMab) within these pathological contexts suggesting interactions that could contribute to disease progression and offering potential targets for therapeutic intervention.
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Specifications

Form

Liquid

Additional notes

Glutathione Sepharose 4 Fast Flow

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General info

Function

May play a role in cell differentiation, proliferation and apoptosis (PubMed : 24556617). Binds cholesterol in cholesterol-containing plasma membrane microdomains and may play a role in the organization of the apical plasma membrane in epithelial cells. During early retinal development acts as a key regulator of disk morphogenesis. Involved in regulation of MAPK and Akt signaling pathways. In neuroblastoma cells suppresses cell differentiation such as neurite outgrowth in a RET-dependent manner (PubMed : 20818439).

Sequence similarities

Belongs to the prominin family.

Post-translational modifications

Isoform 1 and isoform 2 are glycosylated.. Acetylation at Lys-225, Lys-257 and Lys-264 by NAT8 and NAT8B may control PROM1 protein expression and its function in cell apoptosis.

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Product protocols

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Target data

May play a role in cell differentiation, proliferation and apoptosis (PubMed : 24556617). Binds cholesterol in cholesterol-containing plasma membrane microdomains and may play a role in the organization of the apical plasma membrane in epithelial cells. During early retinal development acts as a key regulator of disk morphogenesis. Involved in regulation of MAPK and Akt signaling pathways. In neuroblastoma cells suppresses cell differentiation such as neurite outgrowth in a RET-dependent manner (PubMed : 20818439).
See full target information PROM1
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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Cancer communications (London, England) 42:1008-1027 PubMed35975322

2022

A non-metabolic function of hexokinase 2 in small cell lung cancer: promotes cancer cell stemness by increasing USP11-mediated CD133 stability.

Applications

Unspecified application

Species

Unspecified reactive species

Juhong Wang,Fei Shao,Yannan Yang,Wei Wang,Xueying Yang,Renda Li,Hong Cheng,Sijin Sun,Xiaoli Feng,Yibo Gao,Jie He,Zhimin Lu

Materials today. Bio 15:100332 PubMed35795137

2022

Mechanism assay of interaction between blood vessels-near infrared probe and cell surface marker proteins of endothelial cells.

Applications

Unspecified application

Species

Unspecified reactive species

Muhammad Asri Abdul Sisak,Fiona Louis,Tomoyuki Miyao,Sun Hyeok Lee,Young-Tae Chang,Michiya Matsusaki
View all publications
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