Recombinant human CD134 / OX40L receptor protein (Active) is a Human Fragment protein, in the 29 to 216 aa range, expressed in HEK 293, with >90% purity, < 1 EU/µg endotoxin level and suitable for SDS-PAGE, FuncS.
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Key facts
- Purity
- >90% SDS-PAGE
- Endotoxin level
- < 1 EU/µg
- Expression system
- HEK 293 cells
- Tags
- His tag C-Terminus
- Applications
- SDS-PAGE, FuncS
- Biologically active
- Yes
Amino acid sequence
L H C V G D T Y P S N D R C C H E C R P G N G M V S R C S R S Q N T V C R P C G P G F Y N D V V S S K P C K P C T W C N L R S G S E R K Q L C T A T Q D T V C R C R A G T Q P L D S Y K P G V D C A P C P P G H F S P G D N Q A C K P W T N C T L A G K H T L Q P A S N S S D A I C E D R D P P A T Q P Q E T Q G P P A R P I T V Q P T E A W P R T S Q G P S T R P V E V P G G R A V A
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application SDS-PAGE | Reactivity Reacts | Dilution info - | Notes - |
Application FuncS | Reactivity Reacts | Dilution info - | Notes - |
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Target data
Function
Receptor for TNFSF4/OX40L/GP34. Is a costimulatory molecule implicated in long-term T-cell immunity. (Microbial infection) Acts as a receptor for human herpesvirus 6B/HHV-6B.
Alternative names
CD134, TXGP1L, TNFRSF4, Tumor necrosis factor receptor superfamily member 4, ACT35 antigen, OX40L receptor, TAX transcriptionally-activated glycoprotein 1 receptor
Recommended products
Recombinant human CD134 / OX40L receptor protein (Active) is a Human Fragment protein, in the 29 to 216 aa range, expressed in HEK 293, with >90% purity, < 1 EU/µg endotoxin level and suitable for SDS-PAGE, FuncS.
Key facts
- Purity
- >90% SDS-PAGE
- Endotoxin level
- < 1 EU/µg
- Expression system
- HEK 293 cells
- Tags
- His tag C-Terminus
- Applications
- SDS-PAGE, FuncS
- Biologically active
- Yes
- Biological activity
- Immobilized Human OX40, His Tag at 2 μg/mL (100 μL/well) can bind Human OX40 Ligand, Fc Tag with a linear range of 0.5-16 ng/mL.
- Accession
- P43489-1
- Animal free
- No
- Species
- Human
- Reconstitution
- Reconstitute at 400 µg/mL in water
- Concentration
- Storage buffer
pH: 7.4
Constituents: 95% PBS, 5% Trehalose
Sequence info
Amino acid sequence
- L H C V G D T Y P S N D R C C H E C R P G N G M V S R C S R S Q N T V C R P C G P G F Y N D V V S S K P C K P C T W C N L R S G S E R K Q L C T A T Q D T V C R C R A G T Q P L D S Y K P G V D C A P C P P G H F S P G D N Q A C K P W T N C T L A G K H T L Q P A S N S S D A I C E D R D P P A T Q P Q E T Q G P P A R P I T V Q P T E A W P R T S Q G P S T R P V E V P G G R A V A
- Accession
- P43489
- Protein length
- Fragment
- Predicted molecular weight
- 22 kDa
- Amino acids
- 29 to 216
- Nature
- Recombinant
- Tags
- His tag C-Terminus
Specifications
- Form
- Lyophilized
- Additional notes
Purified by Immobilized metal affinity chromatography.
General info
- Function
Receptor for TNFSF4/OX40L/GP34. Is a costimulatory molecule implicated in long-term T-cell immunity.
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- -20°C
- Appropriate long-term storage conditions
- -20°C
- Storage information
- Avoid freeze / thaw cycle
This product is an active protein and may elicit a biological response in vivo, handle with caution.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
CD134 also known as OX40 or TNFRSF4 is a receptor that plays an essential role in immune response regulation. This receptor is part of the tumor necrosis factor receptor (TNFR) superfamily and has a molecular mass of approximately 50 kDa. CD134 is expressed on activated T cells particularly CD4+ T cells and is present on various other immune cells under specific conditions. The receptor binds to the OX40L its ligand which is expressed on antigen-presenting cells such as dendritic cells and B cells.
- Biological function summary
CD134 influences T cell proliferation survival and memory formation being an important component of the immune system's adaptive response. OX40L engagement with CD134 enhances the activation and survival of T cells promoting long-term immunity. This interaction does not form part of a larger complex but functions as a direct signaling mechanism that augments T cell receptor (TCR) signaling leading to increased cytokine production and sustained immune responses essential for controlling infections and cancer.
- Pathways
CD134 signaling intersects with the nuclear factor-kappa B (NF-κB) and phosphoinositide 3-kinase (PI3K) pathways. These pathways play significant roles in regulating immune responses and cell survival. Through the NF-κB pathway CD134 influences gene transcription related to immune activation. It also connects with other immune regulatory proteins such as CD28 which further potentiates T cell responses and enhances effector functions.
- Associated diseases and disorders
CD134 has connections to autoimmune diseases and cancers. Overactivity of the CD134-OX40L pathway can lead to exacerbated autoimmune conditions such as lupus. This receptor has also been implicated in tumor immunity where its activation enhances the anti-tumor response of T cells. CD134 interacts with CTLA-4 another immune checkpoint protein playing a role in balancing immune activation and regulation within these disease contexts.
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5 product images
Functional Studies - Recombinant human CD134 / OX40L receptor protein (Active) (ab221231)
Loaded Anti-OX40 MAb(Human IgG1) on AHC Biosensor, can bind ab221231 with an affinity constant of 6.03 nM as determined in BLI assay.
Functional Studies - Recombinant human CD134 / OX40L receptor protein (Active) (ab221231)
Loaded ab221412 on AR2G Biosensor, can bind ab221231 with an affinity constant of 0.15 μM as determined in BLI assay.
SDS-PAGE - Recombinant human CD134 / OX40L receptor protein (Active) (ab221231)
ab221231 on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 90%.
Functional Studies - Recombinant human CD134 / OX40L receptor protein (Active) (ab221231)
The purity of ab221231 was more than 85% and around 32-44 kDa verified by SEC-MALS.
Functional Studies - Recombinant human CD134 / OX40L receptor protein (Active) (ab221231)
Immobilized Human OX40, His Tag at 2 µg/mL (100 µL/well)can bind Mouse OX40 Ligand, Fc Tag with a linear range of 0.5-16 ng/mL.
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