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Recombinant human CD134 / OX40L receptor protein (Fc Chimera Active) is a Human Fragment protein, in the 29 to 216 aa range, expressed in HEK 293, with >90% purity, < 1 EU/µg endotoxin level and suitable for SDS-PAGE, FuncS, ELISA.

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Images

ELISA - Recombinant human CD134 / OX40L receptor protein (Fc Chimera Active) (AB221340), expandable thumbnail
  • Functional Studies - Recombinant human CD134 / OX40L receptor protein (Fc Chimera Active) (AB221340), expandable thumbnail
  • SDS-PAGE - Recombinant human CD134 / OX40L receptor protein (Fc Chimera Active) (AB221340), expandable thumbnail

Publications

Key facts

Purity

>90% SDS-PAGE

Endotoxin level
< 1 EU/µg
Expression system

HEK 293 cells

Tags

Fc tag C-Terminus

Applications

SDS-PAGE, FuncS, ELISA

Biologically active

Yes

Amino acid sequence

L H C V G D T Y P S N D R C C H E C R P G N G M V S R C S R S Q N T V C R P C G P G F Y N D V V S S K P C K P C T W C N L R S G S E R K Q L C T A T Q D T V C R C R A G T Q P L D S Y K P G V D C A P C P P G H F S P G D N Q A C K P W T N C T L A G K H T L Q P A S N S S D A I C E D R D P P A T Q P Q E T Q G P P A R P I T V Q P T E A W P R T S Q G P S T R P V E V P G G R A V A P K S C D K T H T C P P C P A P E L L G G P S V F L F P P K P K D T L M I S R T P E V T C V V V D V S H E D P E V K F N W Y V D G V E V H N A K T K P R E E Q Y N S T Y R V V S V L T V L H Q D W L N G K E Y K C K V S N K A L P A P I E K T I S K A K G Q P R E P Q V Y T L P P S R D E L T K N Q V S L T C L V K G F Y P S D I A V E W E S N G Q P E N N Y K T T P P V L D S D G S F F L Y S K L T V D K S R W Q Q G N V F S C S V M H E A L H N H Y T Q K S L S L S P G K

Reactivity data

Application

SDS-PAGE

Reactivity

Reacts

Dilution info
-
Notes

-

Application

FuncS

Reactivity

Reacts

Dilution info
-
Notes

-

Application

ELISA

Reactivity

Reacts

Dilution info
-
Notes

-

Associated Products

Select an associated product type

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Target data

Function

Receptor for TNFSF4/OX40L/GP34. Is a costimulatory molecule implicated in long-term T-cell immunity.(Microbial infection) Acts as a receptor for human herpesvirus 6B/HHV-6B.

Alternative names

Recommended products

Recombinant human CD134 / OX40L receptor protein (Fc Chimera Active) is a Human Fragment protein, in the 29 to 216 aa range, expressed in HEK 293, with >90% purity, < 1 EU/µg endotoxin level and suitable for SDS-PAGE, FuncS, ELISA.

Key facts

Purity

>90% SDS-PAGE

Endotoxin level
< 1 EU/µg
Expression system

HEK 293 cells

Applications

SDS-PAGE, FuncS, ELISA

Biological activity

Measured by its binding ability in a functional ELISA Immobilized Recombinant human OX40L/TNFSF4 protein (His tag) (ab221412) at 5 μg/mL (100 μL/well) can bind ab221340 with a linear range of 2-25 ng/mL.

Accession
P43489-1
Animal free

No

Species

Human

Reconstitution

Reconstitute at 400 µg/mL in water

Concentration
Loading...
Storage buffer

pH: 7.4
Constituents: 5% Trehalose, 0.87% Sodium chloride, 0.75% Glycine, 0.61% Tris, 0.44% L-Arginine

Sequence info

Amino acid sequence

L H C V G D T Y P S N D R C C H E C R P G N G M V S R C S R S Q N T V C R P C G P G F Y N D V V S S K P C K P C T W C N L R S G S E R K Q L C T A T Q D T V C R C R A G T Q P L D S Y K P G V D C A P C P P G H F S P G D N Q A C K P W T N C T L A G K H T L Q P A S N S S D A I C E D R D P P A T Q P Q E T Q G P P A R P I T V Q P T E A W P R T S Q G P S T R P V E V P G G R A V A P K S C D K T H T C P P C P A P E L L G G P S V F L F P P K P K D T L M I S R T P E V T C V V V D V S H E D P E V K F N W Y V D G V E V H N A K T K P R E E Q Y N S T Y R V V S V L T V L H Q D W L N G K E Y K C K V S N K A L P A P I E K T I S K A K G Q P R E P Q V Y T L P P S R D E L T K N Q V S L T C L V K G F Y P S D I A V E W E S N G Q P E N N Y K T T P P V L D S D G S F F L Y S K L T V D K S R W Q Q G N V F S C S V M H E A L H N H Y T Q K S L S L S P G K

Accession
P43489
Protein length

Fragment

Predicted molecular weight

46.8 kDa

Amino acids

29 to 216

Nature

Recombinant

Tags

Fc tag C-Terminus

Specifications

Form

Lyophilized

General info

Function

Receptor for TNFSF4/OX40L/GP34. Is a costimulatory molecule implicated in long-term T-cell immunity.

Storage

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

-20°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

This product is an active protein and may elicit a biological response in vivo, handle with caution.

Notes

This product is stable after storage at:

  • -20°C to -70°C for 12 months in lyophilized state;
  • -70°C for 3 months under sterile conditions after reconstitution.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.

Activity summary

CD134 also known as OX40 or TNFRSF4 is a receptor that plays an essential role in immune response regulation. This receptor is part of the tumor necrosis factor receptor (TNFR) superfamily and has a molecular mass of approximately 50 kDa. CD134 is expressed on activated T cells particularly CD4+ T cells and is present on various other immune cells under specific conditions. The receptor binds to the OX40L its ligand which is expressed on antigen-presenting cells such as dendritic cells and B cells.

Biological function summary

CD134 influences T cell proliferation survival and memory formation being an important component of the immune system's adaptive response. OX40L engagement with CD134 enhances the activation and survival of T cells promoting long-term immunity. This interaction does not form part of a larger complex but functions as a direct signaling mechanism that augments T cell receptor (TCR) signaling leading to increased cytokine production and sustained immune responses essential for controlling infections and cancer.

Pathways

CD134 signaling intersects with the nuclear factor-kappa B (NF-κB) and phosphoinositide 3-kinase (PI3K) pathways. These pathways play significant roles in regulating immune responses and cell survival. Through the NF-κB pathway CD134 influences gene transcription related to immune activation. It also connects with other immune regulatory proteins such as CD28 which further potentiates T cell responses and enhances effector functions.

Associated diseases and disorders

CD134 has connections to autoimmune diseases and cancers. Overactivity of the CD134-OX40L pathway can lead to exacerbated autoimmune conditions such as lupus. This receptor has also been implicated in tumor immunity where its activation enhances the anti-tumor response of T cells. CD134 interacts with CTLA-4 another immune checkpoint protein playing a role in balancing immune activation and regulation within these disease contexts.

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3 product images

  • ELISA - Recombinant human CD134 / OX40L receptor protein (Fc Chimera Active) (ab221340), expandable thumbnail

    ELISA - Recombinant human CD134 / OX40L receptor protein (Fc Chimera Active) (ab221340)

    Immobilized Recombinant human OX40L/TNFSF4 protein (His tag) (ab221412) at 5 μg/mL (100 μL/well) can bind ab221340 with a linear range of 2-25 ng/mL.

  • Functional Studies - Recombinant human CD134 / OX40L receptor protein (Fc Chimera Active) (ab221340), expandable thumbnail

    Functional Studies - Recombinant human CD134 / OX40L receptor protein (Fc Chimera Active) (ab221340)

    FACS assay shows that recombinant Human OX40, Fc Tag can bind to 293T cell overexpressing human OX40L. The concentration of OX40 used is 0.03 µg/ml.

  • SDS-PAGE - Recombinant human CD134 / OX40L receptor protein (Fc Chimera Active) (ab221340), expandable thumbnail

    SDS-PAGE - Recombinant human CD134 / OX40L receptor protein (Fc Chimera Active) (ab221340)

    SDS PAGE analysis of ab221340. The gel was stained overnight with Coomassie Blue. Due to glycosylation, the protein migrates at 65 kDa under reducing conditions.

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Product protocols

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