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Recombinant human CD137 protein (Fc Chimera Active) is a Human Fragment protein, in the 24 to 186 aa range, expressed in HEK 293, with >95% purity, < 1 EU/µg endotoxin level and suitable for SDS-PAGE, FuncS, ELISA, Flow Cyt.
Alternative names=Tumor necrosis factor receptor superfamily member 9, 4-1BB ligand receptor, CDw137, T-cell antigen 4-1BB homolog, T-cell antigen ILA, TNFRSF9, ILA, CD137
- Purity
>95% SDS-PAGE
- Endotoxin level
< 1 EU/µg
- Expression system
HEK 293 cells
- Tags
Fc tag C-Terminus
- Applications
SDS-PAGE, FuncS, ELISA, Flow Cyt
- Biologically active
Yes
- Sequence
L Q D P C S N C P A G T F C D N N R N Q I C S P C P P N S F S S A G G Q R T C D I C R Q C K G V F R T R K E C S S T S N A E C D C T P G F H C L G A G C S M C E Q D C K Q G Q E L T K K G C K D C C F G T F N D Q K R G I C R P W T N C S L D G K S V L V N G T K E R D V V C G P S P A D L S P G A S S V T P P A P A R E P G H S P Q
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application SDS-PAGE | Reactivity Reacts | Dilution info - | Notes - |
Application FuncS | Reactivity Reacts | Dilution info - | Notes - |
Application ELISA | Reactivity Reacts | Dilution info - | Notes - |
Application Flow Cyt | Reactivity Reacts | Dilution info - | Notes - |
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Recombinant human CD137 protein (Fc Chimera Active) is a Human Fragment protein, in the 24 to 186 aa range, expressed in HEK 293, with >95% purity, < 1 EU/µg endotoxin level and suitable for SDS-PAGE, FuncS, ELISA, Flow Cyt.
Alternative names=Tumor necrosis factor receptor superfamily member 9, 4-1BB ligand receptor, CDw137, T-cell antigen 4-1BB homolog, T-cell antigen ILA, TNFRSF9, ILA, CD137
Key facts
- Purity
>95% SDS-PAGE
- Endotoxin level
< 1 EU/µg
- Expression system
HEK 293 cells
- Tags
Fc tag C-Terminus
- Applications
SDS-PAGE, FuncS, ELISA, Flow Cyt
- Biologically active
Yes
- Biological activity
Measured by its binding ability in ELISA. Immobilized ab220584 at 0.1 μg/mL (100 μL/well) can bind Recombinant human 4-1BBL protein (Fc Chimera Active) (Biotin) (ab246043) with a linear range of 0.4-13 ng/mL.
Measured by its binding ability in FACS. Flow Cytometry assay shows that ab220584 can bind to 293T cells overexpressing Human 4-1BBL. The concentration of 4-1BB used is 0.1 μg/mL.
Measured by its binding ability in BLI assay. Loaded ab220584 on Protein A Biosensor, can bind Recombinant human 4-1BBL protein (Fc Chimera Active) (Biotin) (ab246043) with an affinity constant of 1.3 nM.
- Predicted molecular weight
43.4 kDa
- Amino acids
24 to 186
- Sequence
L Q D P C S N C P A G T F C D N N R N Q I C S P C P P N S F S S A G G Q R T C D I C R Q C K G V F R T R K E C S S T S N A E C D C T P G F H C L G A G C S M C E Q D C K Q G Q E L T K K G C K D C C F G T F N D Q K R G I C R P W T N C S L D G K S V L V N G T K E R D V V C G P S P A D L S P G A S S V T P P A P A R E P G H S P Q
- Accession
- Q07011-1
- Protein length
Fragment
- Animal free
No
- Nature
Recombinant
- Species
Human
- Reconstitution
Reconstitute with sterile deionized water to a concentration of 400 µg/ml.
- Concentration
- Storage buffer
pH: 7.4
Constituents: L-Arginine, Sodium chloride, 5% Trehalose, 0.75% Glycine, 0.61% Tris
Specifications
- Form
Lyophilized
General info
- Function
Receptor for TNFSF9/4-1BBL. Possibly active during T cell activation.
Storage
- Shipped at conditions
Blue Ice
- Appropriate long-term storage conditions
-20°C
- Aliquoting information
Upon delivery aliquot
- Storage information
Avoid freeze / thaw cycle
This product is an active protein and may elicit a biological response in vivo, handle with caution.
Notes
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
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