Recombinant human CD14 protein is a Human Full Length protein, in the 20 to 352 aa range, expressed in HEK 293, with >98% purity, < 1 EU/µg endotoxin level and suitable for SDS-PAGE, FuncS.
Images
Key facts
- Purity
>98% SDS-PAGE
- Endotoxin level
< 1 EU/µg
- Expression system
HEK 293 cells
- Tags
His tag C-Terminus
- Applications
SDS-PAGE, FuncS
- Biologically active
Yes
Amino acid sequence
T T P E P C E L D D E D F R C V C N F S E P Q P D W S E A F Q C V S A V E V E I H A G G L N L E P F L K R V D A D A D P R Q Y A D T V K A L R V R R L T V G A A Q V P A Q L L V G A L R V L A Y S R L K E L T L E D L K I T G T M P P L P L E A T G L A L S S L R L R N V S W A T G R S W L A E L Q Q W L K P G L K V L S I A Q A H S P A F S C E Q V R A F P A L T S L D L S D N P G L G E R G L M A A L C P H K F P A I Q N L A L R N T G M E T P T G V C A A L A A A G V Q P H S L D L S H N S L R A T V N P S A P R C M W S S A L N S L N L S F A G L E Q V P K G L P A K L R V L D L S C N R L N R A P Q P D E L P E V D N L T L D G N P F L V P G T A L P H E G S M N S G V V P A C
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application SDS-PAGE | Reactivity Reacts | Dilution info - | Notes Due to different glycosylation, DTT-reduced protein migrates as 45 -50 kDa polypeptide |
Application FuncS | Reactivity Reacts | Dilution info - | Notes - |
Associated Products
Select an associated product type
2 products for Alternative Product
Target data
Function
Coreceptor for bacterial lipopolysaccharide (PubMed:1698311, PubMed:23264655). In concert with LBP, binds to monomeric lipopolysaccharide and delivers it to the LY96/TLR4 complex, thereby mediating the innate immune response to bacterial lipopolysaccharide (LPS) (PubMed:20133493, PubMed:22265692, PubMed:23264655). Acts via MyD88, TIRAP and TRAF6, leading to NF-kappa-B activation, cytokine secretion and the inflammatory response (PubMed:8612135). Acts as a coreceptor for TLR2:TLR6 heterodimer in response to diacylated lipopeptides and for TLR2:TLR1 heterodimer in response to triacylated lipopeptides, these clusters trigger signaling from the cell surface and subsequently are targeted to the Golgi in a lipid-raft dependent pathway (PubMed:16880211). Binds electronegative LDL (LDL(-)) and mediates the cytokine release induced by LDL(-) (PubMed:23880187).
Alternative names
Monocyte differentiation antigen CD14, My23 antigen, Myeloid cell-specific leucine-rich glycoprotein, CD14
Recommended products
Recombinant human CD14 protein is a Human Full Length protein, in the 20 to 352 aa range, expressed in HEK 293, with >98% purity, < 1 EU/µg endotoxin level and suitable for SDS-PAGE, FuncS.
Key facts
- Purity
>98% SDS-PAGE
- Endotoxin level
< 1 EU/µg
- Expression system
HEK 293 cells
- Tags
His tag C-Terminus
- Applications
SDS-PAGE, FuncS
- Biologically active
Yes
- Biological activity
Measured by its ability to enhance LPS stimulated IL8 secretion by THP1 Human acute monocytic leukemia cells. The ED50 for this effect is typically 60-120 ng/ml.
- Accession
- P08571-1
- Animal free
No
- Species
Human
- Reconstitution
Reconstitute the lyophilized protein in sterile deionized water to a final concentration of 200 ug/ml. Solubilize for 30 to 60 minutes at room temperature with occasional gentle mixing. Carrier protein (0.1% HSA or BSA) is strongly recommended for further dilution and long term storage.
- Concentration
- Storage buffer
pH: 7.4
Constituents: 94% PBS, 5% Trehalose
Sequence info
Amino acid sequence
T T P E P C E L D D E D F R C V C N F S E P Q P D W S E A F Q C V S A V E V E I H A G G L N L E P F L K R V D A D A D P R Q Y A D T V K A L R V R R L T V G A A Q V P A Q L L V G A L R V L A Y S R L K E L T L E D L K I T G T M P P L P L E A T G L A L S S L R L R N V S W A T G R S W L A E L Q Q W L K P G L K V L S I A Q A H S P A F S C E Q V R A F P A L T S L D L S D N P G L G E R G L M A A L C P H K F P A I Q N L A L R N T G M E T P T G V C A A L A A A G V Q P H S L D L S H N S L R A T V N P S A P R C M W S S A L N S L N L S F A G L E Q V P K G L P A K L R V L D L S C N R L N R A P Q P D E L P E V D N L T L D G N P F L V P G T A L P H E G S M N S G V V P A C
- Accession
- Protein length
Full Length
- Predicted molecular weight
36.6 kDa
- Amino acids
20 to 352
- Nature
Recombinant
- Tags
His tag C-Terminus
Specifications
- Form
Lyophilized
General info
- Function
Coreceptor for bacterial lipopolysaccharide (PubMed:1698311, PubMed:23264655). In concert with LBP, binds to monomeric lipopolysaccharide and delivers it to the LY96/TLR4 complex, thereby mediating the innate immune response to bacterial lipopolysaccharide (LPS) (PubMed:20133493, PubMed:22265692, PubMed:23264655). Acts via MyD88, TIRAP and TRAF6, leading to NF-kappa-B activation, cytokine secretion and the inflammatory response (PubMed:8612135). Acts as a coreceptor for TLR2:TLR6 heterodimer in response to diacylated lipopeptides and for TLR2:TLR1 heterodimer in response to triacylated lipopeptides, these clusters trigger signaling from the cell surface and subsequently are targeted to the Golgi in a lipid-raft dependent pathway (PubMed:16880211). Binds electronegative LDL (LDL(-)) and mediates the cytokine release induced by LDL(-) (PubMed:23880187).
- Post-translational modifications
N- and O- glycosylated. O-glycosylated with a core 1 or possibly core 8 glycan.
Storage
- Shipped at conditions
Blue Ice
- Appropriate short-term storage duration
1-2 weeks
- Appropriate short-term storage conditions
+4°C
- Appropriate long-term storage conditions
-20°C
- Aliquoting information
Upon delivery aliquot
- Storage information
Avoid freeze / thaw cycle
This product is an active protein and may elicit a biological response in vivo, handle with caution.
Supplementary info
This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
CD14 also known as cluster of differentiation 14 is a glycoprotein with a molecular mass of approximately 55 kDa. This protein is expressed mainly on the surface of macrophages monocytes and to a lesser extent on neutrophils. CD14 exists in two forms: membrane-bound (mCD14) and soluble (sCD14). It functions as a co-receptor along with TLR4 (Toll-like receptor 4) and MD-2 for the detection of bacterial lipopolysaccharide (LPS) which is an important component of the outer membrane of Gram-negative bacteria.
- Biological function summary
This glycoprotein plays a significant role in the innate immune system by recognizing pathogen-associated molecular patterns (PAMPs). It is part of a receptor complex where CD14 acts alongside LPS binding protein (LPS-R) TLR4 and MD-2 to facilitate pro-inflammatory signaling in response to microbial invasion. By binding to LPS CD14 initiates and amplifies the immune response leading to the production of cytokines and the recruitment of other immune cells to the site of infection.
- Pathways
CD14 interacts with the toll-like receptor signaling pathway notably involving TLR4. Upon LPS detection CD14 assists in the activation of TLR4 triggering downstream signaling cascades such as NF-κB and MAPK pathways. These pathways result in the transcription of inflammatory cytokines and are essential for the host defense mechanism. The protein complex involving CD14 further interacts with adaptor proteins like MyD88 and TRIF facilitating signal propagation and the immune system's ability to respond to pathogens.
- Associated diseases and disorders
Researchers have linked CD14 to sepsis and atherosclerosis. High levels of soluble CD14 (sCD14) are often observed in patients with sepsis indicating its participation in excessive systemic inflammation. In atherosclerosis CD14's role in recognizing LPS contributes to chronic inflammation and the development of plaques in arteries. Through these diseases CD14 connects with TLR4 emphasizing its impact on inflammatory responses and its potential as a therapeutic target.
Product promise
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
1 product image
SDS-PAGE - Recombinant human CD14 protein (ab167706)
SDS-PAGE analysis of ab167706.
Downloads
Product protocols
- Visit the general protocols
- Visit troubleshooting
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
For licensing inquiries, please contact partnerships@abcam.com