Recombinant Human CD14 protein
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Recombinant Human CD14 protein is a Human Fragment protein, in the 20 to 335 aa range, expressed in HEK 293 cells, with >95%, <0.005 EU/µg endotoxin level, suitable for SDS-PAGE, Mass Spec, HPLC.
View Alternative Names
CD14, Monocyte differentiation antigen CD14, My23 antigen, Myeloid cell-specific leucine-rich glycoprotein
- Mass Spec
Supplier Data
Mass Spectrometry - Recombinant Human CD14 protein (AB282377)
Mass determination by ESI-TOF.
Predicted MW is 34238.23 Da (+/- 10 Da by ESI-TOF). Observed MW is 34241.39 Da.
- SDS-PAGE
Supplier Data
SDS-PAGE - Recombinant Human CD14 protein (AB282377)
SDS-PAGE analysis of ab282377.
- HPLC
Supplier Data
HPLC - Recombinant Human CD14 protein (AB282377)
HPLC analysis of ab282377.
Complementary Products
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD14 antibody [4B4F12] (AB182032)](https://content.abcam.com/products/images/cd14-antibody-4b4f12-ab182032--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img132352.jpg)
Reactivity data
Sequence info
Properties and storage information
Shipped at conditions
Appropriate long-term storage conditions
Supplementary information
This supplementary information is collated from multiple sources and compiled automatically.
Biological function summary
This glycoprotein plays a significant role in the innate immune system by recognizing pathogen-associated molecular patterns (PAMPs). It is part of a receptor complex where CD14 acts alongside LPS binding protein (LPS-R) TLR4 and MD-2 to facilitate pro-inflammatory signaling in response to microbial invasion. By binding to LPS CD14 initiates and amplifies the immune response leading to the production of cytokines and the recruitment of other immune cells to the site of infection.
Pathways
CD14 interacts with the toll-like receptor signaling pathway notably involving TLR4. Upon LPS detection CD14 assists in the activation of TLR4 triggering downstream signaling cascades such as NF-κB and MAPK pathways. These pathways result in the transcription of inflammatory cytokines and are essential for the host defense mechanism. The protein complex involving CD14 further interacts with adaptor proteins like MyD88 and TRIF facilitating signal propagation and the immune system's ability to respond to pathogens.
Specifications
Form
Lyophilized
Additional notes
>95% by HPLC
General info
Function
Coreceptor for bacterial lipopolysaccharide (PubMed : 1698311, PubMed : 23264655). In concert with LBP, binds to monomeric lipopolysaccharide and delivers it to the LY96/TLR4 complex, thereby mediating the innate immune response to bacterial lipopolysaccharide (LPS) (PubMed : 20133493, PubMed : 22265692, PubMed : 23264655). Acts via MyD88, TIRAP and TRAF6, leading to NF-kappa-B activation, cytokine secretion and the inflammatory response (PubMed : 8612135). Acts as a coreceptor for TLR2 : TLR6 heterodimer in response to diacylated lipopeptides and for TLR2 : TLR1 heterodimer in response to triacylated lipopeptides, these clusters trigger signaling from the cell surface and subsequently are targeted to the Golgi in a lipid-raft dependent pathway (PubMed : 16880211). Binds electronegative LDL (LDL(-)) and mediates the cytokine release induced by LDL(-) (PubMed : 23880187).
Post-translational modifications
N- and O- glycosylated. O-glycosylated with a core 1 or possibly core 8 glycan.
Target data
Product promise
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